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Isolation and characterization of a Haemophilus paragallinarum mutant that lacks a hemagglutinating antigen.

Abstract

In an in vivo cross-protection test with Haemophilus paragallinarum strains of serovars B and C, we isolated and characterized a mutant strain, S1M, which lacked a hemagglutinating (HA) antigen when compared biologically and immunologically with isogenic strain S1. Unlike the isogenic strain S1, the mutant strain S1M lacked HA activity against formaldehyde-fixed chicken erythrocytes, even after hyaluronidase treatment, and it did not stimulate hemagglutination-inhibition antibody in chickens immunized with bacterial cells. Dot-blot testing and immunoelectron microscopy with monoclonal antibodies against serovar C-specific HA antigens showed that strain S1M did not react with these monoclonal antibodies. Furthermore, strain S1M was found to be both non-pathogenic and non-immunogenic. In contrast, the isogenic strain S1 reacted with these monoclonal antibodies and was pathogenic and immunogenic. These results suggest that the HA antigen of H. paragallinarum serovar C. strain plays an important role in pathogenicity and immunogenicity.

Authors+Show Affiliations

,

Aburahi Laboratories, Shionogi Research Laboratories, Shionogi & Co., Ltd., Shiga, Japan.

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Source

Avian diseases 37:4 pg 970-6

MeSH

Animals
Antibodies, Monoclonal
Bacterial Outer Membrane Proteins
Chickens
Chromosomes, Bacterial
DNA, Bacterial
Erythrocytes
Haemophilus
Haemophilus Infections
Hemagglutination Tests
Immunoblotting
Microscopy, Immunoelectron
Mutation
Poultry Diseases
Serotyping

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8141756

Citation

Yamaguchi, T, et al. "Isolation and Characterization of a Haemophilus Paragallinarum Mutant That Lacks a Hemagglutinating Antigen." Avian Diseases, vol. 37, no. 4, 1993, pp. 970-6.
Yamaguchi T, Kobayashi M, Masaki S, et al. Isolation and characterization of a Haemophilus paragallinarum mutant that lacks a hemagglutinating antigen. Avian Dis. 1993;37(4):970-6.
Yamaguchi, T., Kobayashi, M., Masaki, S., & Iritani, Y. (1993). Isolation and characterization of a Haemophilus paragallinarum mutant that lacks a hemagglutinating antigen. Avian Diseases, 37(4), pp. 970-6.
Yamaguchi T, et al. Isolation and Characterization of a Haemophilus Paragallinarum Mutant That Lacks a Hemagglutinating Antigen. Avian Dis. 1993;37(4):970-6. PubMed PMID: 8141756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of a Haemophilus paragallinarum mutant that lacks a hemagglutinating antigen. AU - Yamaguchi,T, AU - Kobayashi,M, AU - Masaki,S, AU - Iritani,Y, PY - 1993/10/1/pubmed PY - 1993/10/1/medline PY - 1993/10/1/entrez SP - 970 EP - 6 JF - Avian diseases JO - Avian Dis. VL - 37 IS - 4 N2 - In an in vivo cross-protection test with Haemophilus paragallinarum strains of serovars B and C, we isolated and characterized a mutant strain, S1M, which lacked a hemagglutinating (HA) antigen when compared biologically and immunologically with isogenic strain S1. Unlike the isogenic strain S1, the mutant strain S1M lacked HA activity against formaldehyde-fixed chicken erythrocytes, even after hyaluronidase treatment, and it did not stimulate hemagglutination-inhibition antibody in chickens immunized with bacterial cells. Dot-blot testing and immunoelectron microscopy with monoclonal antibodies against serovar C-specific HA antigens showed that strain S1M did not react with these monoclonal antibodies. Furthermore, strain S1M was found to be both non-pathogenic and non-immunogenic. In contrast, the isogenic strain S1 reacted with these monoclonal antibodies and was pathogenic and immunogenic. These results suggest that the HA antigen of H. paragallinarum serovar C. strain plays an important role in pathogenicity and immunogenicity. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/8141756/Isolation_and_characterization_of_a_Haemophilus_paragallinarum_mutant_that_lacks_a_hemagglutinating_antigen_ L2 - https://medlineplus.gov/haemophilusinfections.html DB - PRIME DP - Unbound Medicine ER -