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Detection of chimeric BCR-ABL genes on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridization.
Blood 1994; 83(7):1922-8Blood

Abstract

The presence of BCR-ABL fusion genes has important diagnostic and prognostic implications in chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). The CML-specific chimeric BCR-ABL gene with a break involving the major breakpoint cluster region (M-bcr) of the BCR-gene has been detected by means of fluorescence in situ hybridization (FISH). In this study, we present a FISH protocol that allows the detection of breaks in both the major and the minor breakpoint cluster region (m-bcr). Three hybridization signals of D107F9, a yeast artificial chromosome (YAC)-derived probe spanning the breakpoint regions of the BCR gene, were indicative of the translocation events. To increase the specificity further, this probe was combined with cos-abl 8, a cosmid probe flanking the breakpoint within the ABL gene for dual-color hybridization. Samples of 21 patients with CML, the ALL-derived cell line SUP-B15, and of seven patients with Philadelphia chromosome (Ph1)-positive ALL (three of them with breakpoints within m-bcr) were examined. BCR-ABL fusion was detected in all cases with high specificity (false-positive nuclei: mean, 0.1%). On cytogenetic preparations, the percentages of BCR-ABL-positive interphase cells ranged from 53% to 91%. Comparable efficiencies were achieved on blood smears. In conclusion, hybridization with D107F9 and cos-abl 8 allows unambiguous diagnosis of BCR-ABL genes and is likely to become an important tool for the monitoring of therapies in patients with CML and ALL.

Authors+Show Affiliations

Medizinische Klinik, Universität Heidelberg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8142658

Citation

Bentz, M, et al. "Detection of Chimeric BCR-ABL Genes On Bone Marrow Samples and Blood Smears in Chronic Myeloid and Acute Lymphoblastic Leukemia By in Situ Hybridization." Blood, vol. 83, no. 7, 1994, pp. 1922-8.
Bentz M, Cabot G, Moos M, et al. Detection of chimeric BCR-ABL genes on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridization. Blood. 1994;83(7):1922-8.
Bentz, M., Cabot, G., Moos, M., Speicher, M. R., Ganser, A., Lichter, P., & Döhner, H. (1994). Detection of chimeric BCR-ABL genes on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridization. Blood, 83(7), pp. 1922-8.
Bentz M, et al. Detection of Chimeric BCR-ABL Genes On Bone Marrow Samples and Blood Smears in Chronic Myeloid and Acute Lymphoblastic Leukemia By in Situ Hybridization. Blood. 1994 Apr 1;83(7):1922-8. PubMed PMID: 8142658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of chimeric BCR-ABL genes on bone marrow samples and blood smears in chronic myeloid and acute lymphoblastic leukemia by in situ hybridization. AU - Bentz,M, AU - Cabot,G, AU - Moos,M, AU - Speicher,M R, AU - Ganser,A, AU - Lichter,P, AU - Döhner,H, PY - 1994/4/1/pubmed PY - 1994/4/1/medline PY - 1994/4/1/entrez SP - 1922 EP - 8 JF - Blood JO - Blood VL - 83 IS - 7 N2 - The presence of BCR-ABL fusion genes has important diagnostic and prognostic implications in chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). The CML-specific chimeric BCR-ABL gene with a break involving the major breakpoint cluster region (M-bcr) of the BCR-gene has been detected by means of fluorescence in situ hybridization (FISH). In this study, we present a FISH protocol that allows the detection of breaks in both the major and the minor breakpoint cluster region (m-bcr). Three hybridization signals of D107F9, a yeast artificial chromosome (YAC)-derived probe spanning the breakpoint regions of the BCR gene, were indicative of the translocation events. To increase the specificity further, this probe was combined with cos-abl 8, a cosmid probe flanking the breakpoint within the ABL gene for dual-color hybridization. Samples of 21 patients with CML, the ALL-derived cell line SUP-B15, and of seven patients with Philadelphia chromosome (Ph1)-positive ALL (three of them with breakpoints within m-bcr) were examined. BCR-ABL fusion was detected in all cases with high specificity (false-positive nuclei: mean, 0.1%). On cytogenetic preparations, the percentages of BCR-ABL-positive interphase cells ranged from 53% to 91%. Comparable efficiencies were achieved on blood smears. In conclusion, hybridization with D107F9 and cos-abl 8 allows unambiguous diagnosis of BCR-ABL genes and is likely to become an important tool for the monitoring of therapies in patients with CML and ALL. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/8142658/Detection_of_chimeric_BCR_ABL_genes_on_bone_marrow_samples_and_blood_smears_in_chronic_myeloid_and_acute_lymphoblastic_leukemia_by_in_situ_hybridization_ L2 - http://www.bloodjournal.org/cgi/pmidlookup?view=long&pmid=8142658 DB - PRIME DP - Unbound Medicine ER -