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Coronavirus transmissible gastroenteritis virus-mediated induction of IFN alpha-mRNA in porcine leukocytes requires prior synthesis of soluble proteins.
Vet Res 1994; 25(1):29-36VR

Abstract

We studied the expression of interferon alpha (IFN alpha)-mRNA in porcine non-adherent peripheral blood mononuclear cells (PBMC) after induction by the coronavirus transmissible gastroenteritis virus (TGEV). We found that protein synthesis inhibition by cycloheximide (CHX) blocked IFN alpha-mRNA expression, except when PBMC were preincubated with a conditioned medium as a potential source of cytokines. These data indicate that IFN alpha-mRNA induction by TGEV requires de novo synthesis of proteins. Moreover, they suggest that IFN alpha-mRNA induction in porcine leukocytes by TGEV involves mechanisms identical to those described for the herpes simplex virus in humans. In addition, experiments performed with a TGEV mutant, dm 49-4, previously characterized for its low ability to induce IFN alpha, showed that addition of a conditioned medium could not normalize its IFN alpha-inducing ability. Therefore, the defect of the dm49-4 mutant may be at the level of the final triggering signal to PBMC.

Authors+Show Affiliations

INRA, Laboratorie de Virologie et Immunologie Moléculaires, Jouy-en-Josas, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8142954

Citation

Charley, B, et al. "Coronavirus Transmissible Gastroenteritis Virus-mediated Induction of IFN alpha-mRNA in Porcine Leukocytes Requires Prior Synthesis of Soluble Proteins." Veterinary Research, vol. 25, no. 1, 1994, pp. 29-36.
Charley B, Lavenant L, Lefèvre F. Coronavirus transmissible gastroenteritis virus-mediated induction of IFN alpha-mRNA in porcine leukocytes requires prior synthesis of soluble proteins. Vet Res. 1994;25(1):29-36.
Charley, B., Lavenant, L., & Lefèvre, F. (1994). Coronavirus transmissible gastroenteritis virus-mediated induction of IFN alpha-mRNA in porcine leukocytes requires prior synthesis of soluble proteins. Veterinary Research, 25(1), pp. 29-36.
Charley B, Lavenant L, Lefèvre F. Coronavirus Transmissible Gastroenteritis Virus-mediated Induction of IFN alpha-mRNA in Porcine Leukocytes Requires Prior Synthesis of Soluble Proteins. Vet Res. 1994;25(1):29-36. PubMed PMID: 8142954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coronavirus transmissible gastroenteritis virus-mediated induction of IFN alpha-mRNA in porcine leukocytes requires prior synthesis of soluble proteins. AU - Charley,B, AU - Lavenant,L, AU - Lefèvre,F, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 29 EP - 36 JF - Veterinary research JO - Vet. Res. VL - 25 IS - 1 N2 - We studied the expression of interferon alpha (IFN alpha)-mRNA in porcine non-adherent peripheral blood mononuclear cells (PBMC) after induction by the coronavirus transmissible gastroenteritis virus (TGEV). We found that protein synthesis inhibition by cycloheximide (CHX) blocked IFN alpha-mRNA expression, except when PBMC were preincubated with a conditioned medium as a potential source of cytokines. These data indicate that IFN alpha-mRNA induction by TGEV requires de novo synthesis of proteins. Moreover, they suggest that IFN alpha-mRNA induction in porcine leukocytes by TGEV involves mechanisms identical to those described for the herpes simplex virus in humans. In addition, experiments performed with a TGEV mutant, dm 49-4, previously characterized for its low ability to induce IFN alpha, showed that addition of a conditioned medium could not normalize its IFN alpha-inducing ability. Therefore, the defect of the dm49-4 mutant may be at the level of the final triggering signal to PBMC. SN - 0928-4249 UR - https://www.unboundmedicine.com/medline/citation/8142954/Coronavirus_transmissible_gastroenteritis_virus_mediated_induction_of_IFN_alpha_mRNA_in_porcine_leukocytes_requires_prior_synthesis_of_soluble_proteins_ DB - PRIME DP - Unbound Medicine ER -