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Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a geriatric hospital.
J Clin Microbiol. 1994 Feb; 32(2):301-5.JC

Abstract

Twelve cases of infections caused by extended-spectrum beta-lactamase (ESBla)-producing Klebsiella pneumoniae were reported between August 1991 and March 1993 in the Geriatric Department of the Nimes University Hospital, where these bacterial had not been previously isolated. Restriction profiles of total genomic DNAs cleaved by XbaI and SpeI were compared by pulsed-field gel electrophoresis. The strains that were tested included the 12 isolates from K. pneumoniae-infected patients, strains recovered from rectal swabs of asymptomatic patients in the same ward, and strains isolated in other hospitals in Nîmes at the same time. The restriction profiles of the 12 isolates and those recovered from asymptomatic patients in the same ward were very similar. Over a period of more than 1 year, extended-spectrum beta-lactamases were not detected in K. pneumoniae isolates with restriction patterns different from that of the epidemic strain. It seems, therefore, that there was no transfer of a plasmid or a gene coding for ESBla to strains of K. pneumoniae that were different from the epidemic strain. At the same time, ESBla-producing K. pneumoniae isolates exhibiting restriction endonuclease profiles very different from that of the epidemic strain were isolated from other hospitals in Nîmes. None of these strains caused an outbreak. Pulsed-field gel electrophoresis, which allows precise characterization of strains beyond the species level, is a useful tool for studying the ESBla-producing K. pneumoniae strains involved in nosocomial outbreaks.

Authors+Show Affiliations

Faculté de Médecine, Institut National de la Santé et de la Recherche Médicale, Unité 65, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8150938

Citation

Gouby, A, et al. "Epidemiological Study By Pulsed-field Gel Electrophoresis of an Outbreak of Extended-spectrum Beta-lactamase-producing Klebsiella Pneumoniae in a Geriatric Hospital." Journal of Clinical Microbiology, vol. 32, no. 2, 1994, pp. 301-5.
Gouby A, Neuwirth C, Bourg G, et al. Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a geriatric hospital. J Clin Microbiol. 1994;32(2):301-5.
Gouby, A., Neuwirth, C., Bourg, G., Bouziges, N., Carles-Nurit, M. J., Despaux, E., & Ramuz, M. (1994). Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a geriatric hospital. Journal of Clinical Microbiology, 32(2), 301-5.
Gouby A, et al. Epidemiological Study By Pulsed-field Gel Electrophoresis of an Outbreak of Extended-spectrum Beta-lactamase-producing Klebsiella Pneumoniae in a Geriatric Hospital. J Clin Microbiol. 1994;32(2):301-5. PubMed PMID: 8150938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epidemiological study by pulsed-field gel electrophoresis of an outbreak of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a geriatric hospital. AU - Gouby,A, AU - Neuwirth,C, AU - Bourg,G, AU - Bouziges,N, AU - Carles-Nurit,M J, AU - Despaux,E, AU - Ramuz,M, PY - 1994/2/1/pubmed PY - 1994/2/1/medline PY - 1994/2/1/entrez SP - 301 EP - 5 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 32 IS - 2 N2 - Twelve cases of infections caused by extended-spectrum beta-lactamase (ESBla)-producing Klebsiella pneumoniae were reported between August 1991 and March 1993 in the Geriatric Department of the Nimes University Hospital, where these bacterial had not been previously isolated. Restriction profiles of total genomic DNAs cleaved by XbaI and SpeI were compared by pulsed-field gel electrophoresis. The strains that were tested included the 12 isolates from K. pneumoniae-infected patients, strains recovered from rectal swabs of asymptomatic patients in the same ward, and strains isolated in other hospitals in Nîmes at the same time. The restriction profiles of the 12 isolates and those recovered from asymptomatic patients in the same ward were very similar. Over a period of more than 1 year, extended-spectrum beta-lactamases were not detected in K. pneumoniae isolates with restriction patterns different from that of the epidemic strain. It seems, therefore, that there was no transfer of a plasmid or a gene coding for ESBla to strains of K. pneumoniae that were different from the epidemic strain. At the same time, ESBla-producing K. pneumoniae isolates exhibiting restriction endonuclease profiles very different from that of the epidemic strain were isolated from other hospitals in Nîmes. None of these strains caused an outbreak. Pulsed-field gel electrophoresis, which allows precise characterization of strains beyond the species level, is a useful tool for studying the ESBla-producing K. pneumoniae strains involved in nosocomial outbreaks. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/8150938/Epidemiological_study_by_pulsed_field_gel_electrophoresis_of_an_outbreak_of_extended_spectrum_beta_lactamase_producing_Klebsiella_pneumoniae_in_a_geriatric_hospital_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=8150938 DB - PRIME DP - Unbound Medicine ER -