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Phospholipid headgroup-headgroup electrostatic interactions in mixed bilayers of cardiolipin with phosphatidylcholines studied by 2H NMR.
Biochemistry. 1994 Apr 26; 33(16):4896-902.B

Abstract

The headgroup-headgroup interactions in binary mixed bilayers of diacylphosphatidylcholines (PC) and cardiolipin were analyzed by 2H NMR. Specific changes in the quadrupole splittings of the choline headgroup deuterated PC at alpha,beta-methylenes, and gamma-methyls are observed upon the insertion of the negatively charged tetraacylphospholipid, cardiolipin. The effects are consistent with an electrostatic interaction between PC and cardiolipin headgroups, in which a concerted conformational reorientation of the entire phosphocholine moiety toward the membrane surface is involved. On the basis of the "choline-tilt" model by Macdonald and co-workers (1991) the variations in the quadrupole splittings are consistent with a change in orientation of the choline P-N vector up to 23 degrees for the highest cardiolipin concentrations. Additional information on headgroup conformational changes was obtained through the analysis of the dependence on temperature of the quadrupole splittings for the various deuterium-labeled segments. Evaluation of the deuterium spin-lattice (T1) relaxation times for the deuterons in the various positions of the choline headgroup in mixed bilayers of PC and cardiolipin showed that the internal fast segmental motions were not affected on addition of cardiolipin to PC membranes.

Authors+Show Affiliations

Pinheiro TJ
Department of Biochemistry, University of Oxford, U.K.
Duralski AA
No affiliation info available
Watts A
No affiliation info available

MeSH

CardiolipinsElectrochemistryLipid BilayersMagnetic Resonance SpectroscopyModels, ChemicalMolecular ConformationPhosphatidylcholines

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8161549

Citation

Pinheiro, T J., et al. "Phospholipid Headgroup-headgroup Electrostatic Interactions in Mixed Bilayers of Cardiolipin With Phosphatidylcholines Studied By 2H NMR." Biochemistry, vol. 33, no. 16, 1994, pp. 4896-902.
Pinheiro TJ, Duralski AA, Watts A. Phospholipid headgroup-headgroup electrostatic interactions in mixed bilayers of cardiolipin with phosphatidylcholines studied by 2H NMR. Biochemistry. 1994;33(16):4896-902.
Pinheiro, T. J., Duralski, A. A., & Watts, A. (1994). Phospholipid headgroup-headgroup electrostatic interactions in mixed bilayers of cardiolipin with phosphatidylcholines studied by 2H NMR. Biochemistry, 33(16), 4896-902.
Pinheiro TJ, Duralski AA, Watts A. Phospholipid Headgroup-headgroup Electrostatic Interactions in Mixed Bilayers of Cardiolipin With Phosphatidylcholines Studied By 2H NMR. Biochemistry. 1994 Apr 26;33(16):4896-902. PubMed PMID: 8161549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phospholipid headgroup-headgroup electrostatic interactions in mixed bilayers of cardiolipin with phosphatidylcholines studied by 2H NMR. AU - Pinheiro,T J, AU - Duralski,A A, AU - Watts,A, PY - 1994/4/26/pubmed PY - 1994/4/26/medline PY - 1994/4/26/entrez SP - 4896 EP - 902 JF - Biochemistry JO - Biochemistry VL - 33 IS - 16 N2 - The headgroup-headgroup interactions in binary mixed bilayers of diacylphosphatidylcholines (PC) and cardiolipin were analyzed by 2H NMR. Specific changes in the quadrupole splittings of the choline headgroup deuterated PC at alpha,beta-methylenes, and gamma-methyls are observed upon the insertion of the negatively charged tetraacylphospholipid, cardiolipin. The effects are consistent with an electrostatic interaction between PC and cardiolipin headgroups, in which a concerted conformational reorientation of the entire phosphocholine moiety toward the membrane surface is involved. On the basis of the "choline-tilt" model by Macdonald and co-workers (1991) the variations in the quadrupole splittings are consistent with a change in orientation of the choline P-N vector up to 23 degrees for the highest cardiolipin concentrations. Additional information on headgroup conformational changes was obtained through the analysis of the dependence on temperature of the quadrupole splittings for the various deuterium-labeled segments. Evaluation of the deuterium spin-lattice (T1) relaxation times for the deuterons in the various positions of the choline headgroup in mixed bilayers of PC and cardiolipin showed that the internal fast segmental motions were not affected on addition of cardiolipin to PC membranes. SN - 0006-2960 UR - https://www.unboundmedicine.com/medline/citation/8161549/Phospholipid_headgroup_headgroup_electrostatic_interactions_in_mixed_bilayers_of_cardiolipin_with_phosphatidylcholines_studied_by_2H_NMR_ DB - PRIME DP - Unbound Medicine ER -