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Changes in fibre-type composition and myosin heavy-chain IId isoform in rat soleus muscle during recovery period after hindlimb suspension.
Eur J Appl Physiol Occup Physiol. 1994; 68(1):102-6.EJ

Abstract

We examined the changes in myosin heavy-chain (HC) isoforms and fibre-type composition in rat soleus muscle using both myosin adenosine triphosphatase staining and sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS-PAGE) analyses during the recovery period after 4 weeks of hindlimb suspension. Although there was no change in type IIc fibres after the suspension, an increase in this type of fibres was observed during the 1- to 4-week recovery period. The increase in type IIc fibres was considered to be due to a shift from type IIa to IIc fibres. The SDS-PAGE analysis revealed the presence of the HC IId isoform, which was not observed in the control muscle, after a 4-week hindlimb suspension. The HC IId isoform gradually decreased over 3 weeks of recovery and disappeared in the 4th week of recovery after the suspension. These results suggest that the hypogravity conditions induced by hindlimb suspension stimulated the synthesis of the HC IId isoform, whereas an increase in mechanical load to the muscle accelerated the degradation of the HC IId isoform and the synthesis of type IIc fibres during the recovery period after hindlimb suspension.

Authors+Show Affiliations

Department of Physical Education, Faculty of General Education, Kumamoto University, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8162916

Citation

Oishi, Y, et al. "Changes in Fibre-type Composition and Myosin Heavy-chain IId Isoform in Rat Soleus Muscle During Recovery Period After Hindlimb Suspension." European Journal of Applied Physiology and Occupational Physiology, vol. 68, no. 1, 1994, pp. 102-6.
Oishi Y, Yamamoto H, Miyamoto E. Changes in fibre-type composition and myosin heavy-chain IId isoform in rat soleus muscle during recovery period after hindlimb suspension. Eur J Appl Physiol Occup Physiol. 1994;68(1):102-6.
Oishi, Y., Yamamoto, H., & Miyamoto, E. (1994). Changes in fibre-type composition and myosin heavy-chain IId isoform in rat soleus muscle during recovery period after hindlimb suspension. European Journal of Applied Physiology and Occupational Physiology, 68(1), 102-6.
Oishi Y, Yamamoto H, Miyamoto E. Changes in Fibre-type Composition and Myosin Heavy-chain IId Isoform in Rat Soleus Muscle During Recovery Period After Hindlimb Suspension. Eur J Appl Physiol Occup Physiol. 1994;68(1):102-6. PubMed PMID: 8162916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in fibre-type composition and myosin heavy-chain IId isoform in rat soleus muscle during recovery period after hindlimb suspension. AU - Oishi,Y, AU - Yamamoto,H, AU - Miyamoto,E, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 102 EP - 6 JF - European journal of applied physiology and occupational physiology JO - Eur J Appl Physiol Occup Physiol VL - 68 IS - 1 N2 - We examined the changes in myosin heavy-chain (HC) isoforms and fibre-type composition in rat soleus muscle using both myosin adenosine triphosphatase staining and sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS-PAGE) analyses during the recovery period after 4 weeks of hindlimb suspension. Although there was no change in type IIc fibres after the suspension, an increase in this type of fibres was observed during the 1- to 4-week recovery period. The increase in type IIc fibres was considered to be due to a shift from type IIa to IIc fibres. The SDS-PAGE analysis revealed the presence of the HC IId isoform, which was not observed in the control muscle, after a 4-week hindlimb suspension. The HC IId isoform gradually decreased over 3 weeks of recovery and disappeared in the 4th week of recovery after the suspension. These results suggest that the hypogravity conditions induced by hindlimb suspension stimulated the synthesis of the HC IId isoform, whereas an increase in mechanical load to the muscle accelerated the degradation of the HC IId isoform and the synthesis of type IIc fibres during the recovery period after hindlimb suspension. SN - 0301-5548 UR - https://www.unboundmedicine.com/medline/citation/8162916/Changes_in_fibre_type_composition_and_myosin_heavy_chain_IId_isoform_in_rat_soleus_muscle_during_recovery_period_after_hindlimb_suspension_ DB - PRIME DP - Unbound Medicine ER -