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Alveolar macrophages from patients with beryllium disease and sarcoidosis express increased levels of mRNA for tumor necrosis factor-alpha and interleukin-6 but not interleukin-1 beta.
Am J Respir Cell Mol Biol. 1994 May; 10(5):506-13.AJ

Abstract

Recent evidence suggests that the alveolar macrophage-derived cytokines tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6) play important roles in granulomatous diseases. Our objective was to quantify the mRNA for these cytokines in beryllium disease, a human granulomatous disease of known etiology. We hypothesized that alveolar macrophages and bronchoalveolar lavage fluid from patients with beryllium disease and sarcoidosis would express increased levels of mRNA and proteins, respectively, for TNF-alpha, IL-1 beta, and IL-6 compared with those of normal individuals. We performed bronchoalveolar lavage and used a quantitative polymerase chain reaction to determine alveolar macrophage-derived cytokine gene expression. We determined lavage fluid cytokine levels by enzyme-linked immunosorbent assay. In patients with beryllium disease (n = 23), we observed elevated macrophage mRNA expression for TNF-alpha and IL-6 when compared with that of normal subjects (n = 7). Sarcoidosis patients (n = 6) also had increased expression for TNF-alpha and IL-6 compared with that of normal volunteers. IL-1 beta expression was similar in all three groups. In patients with beryllium disease (n = 39), lavage fluid TNF-alpha concentration was higher than that of 16 normal subjects. Lavage fluid IL-1 beta and IL-6 levels did not differ among the groups. This is the first report of macrophage cytokine expression in beryllium disease. These novel findings suggest that macrophage expression of TNF-alpha and IL-6 may be important in the human granulomatous inflammatory response.

Authors+Show Affiliations

Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8179912

Citation

Bost, T W., et al. "Alveolar Macrophages From Patients With Beryllium Disease and Sarcoidosis Express Increased Levels of mRNA for Tumor Necrosis Factor-alpha and Interleukin-6 but Not Interleukin-1 Beta." American Journal of Respiratory Cell and Molecular Biology, vol. 10, no. 5, 1994, pp. 506-13.
Bost TW, Riches DW, Schumacher B, et al. Alveolar macrophages from patients with beryllium disease and sarcoidosis express increased levels of mRNA for tumor necrosis factor-alpha and interleukin-6 but not interleukin-1 beta. Am J Respir Cell Mol Biol. 1994;10(5):506-13.
Bost, T. W., Riches, D. W., Schumacher, B., Carré, P. C., Khan, T. Z., Martinez, J. A., & Newman, L. S. (1994). Alveolar macrophages from patients with beryllium disease and sarcoidosis express increased levels of mRNA for tumor necrosis factor-alpha and interleukin-6 but not interleukin-1 beta. American Journal of Respiratory Cell and Molecular Biology, 10(5), 506-13.
Bost TW, et al. Alveolar Macrophages From Patients With Beryllium Disease and Sarcoidosis Express Increased Levels of mRNA for Tumor Necrosis Factor-alpha and Interleukin-6 but Not Interleukin-1 Beta. Am J Respir Cell Mol Biol. 1994;10(5):506-13. PubMed PMID: 8179912.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alveolar macrophages from patients with beryllium disease and sarcoidosis express increased levels of mRNA for tumor necrosis factor-alpha and interleukin-6 but not interleukin-1 beta. AU - Bost,T W, AU - Riches,D W, AU - Schumacher,B, AU - Carré,P C, AU - Khan,T Z, AU - Martinez,J A, AU - Newman,L S, PY - 1994/5/1/pubmed PY - 1994/5/1/medline PY - 1994/5/1/entrez SP - 506 EP - 13 JF - American journal of respiratory cell and molecular biology JO - Am J Respir Cell Mol Biol VL - 10 IS - 5 N2 - Recent evidence suggests that the alveolar macrophage-derived cytokines tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-6 (IL-6) play important roles in granulomatous diseases. Our objective was to quantify the mRNA for these cytokines in beryllium disease, a human granulomatous disease of known etiology. We hypothesized that alveolar macrophages and bronchoalveolar lavage fluid from patients with beryllium disease and sarcoidosis would express increased levels of mRNA and proteins, respectively, for TNF-alpha, IL-1 beta, and IL-6 compared with those of normal individuals. We performed bronchoalveolar lavage and used a quantitative polymerase chain reaction to determine alveolar macrophage-derived cytokine gene expression. We determined lavage fluid cytokine levels by enzyme-linked immunosorbent assay. In patients with beryllium disease (n = 23), we observed elevated macrophage mRNA expression for TNF-alpha and IL-6 when compared with that of normal subjects (n = 7). Sarcoidosis patients (n = 6) also had increased expression for TNF-alpha and IL-6 compared with that of normal volunteers. IL-1 beta expression was similar in all three groups. In patients with beryllium disease (n = 39), lavage fluid TNF-alpha concentration was higher than that of 16 normal subjects. Lavage fluid IL-1 beta and IL-6 levels did not differ among the groups. This is the first report of macrophage cytokine expression in beryllium disease. These novel findings suggest that macrophage expression of TNF-alpha and IL-6 may be important in the human granulomatous inflammatory response. SN - 1044-1549 UR - https://www.unboundmedicine.com/medline/citation/8179912/Alveolar_macrophages_from_patients_with_beryllium_disease_and_sarcoidosis_express_increased_levels_of_mRNA_for_tumor_necrosis_factor_alpha_and_interleukin_6_but_not_interleukin_1_beta_ L2 - https://www.atsjournals.org/doi/10.1165/ajrcmb.10.5.8179912?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -