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Testosterone feedback on gonadotropin secretion and gene expression in transgenic mice expressing human growth hormone gene.
J Androl. 1994 Jan-Feb; 15(1):9-14.JA

Abstract

To determine the effects of testosterone on the regulation of gonadotropins in metallothionein-1/human growth hormone (MT/hGH) transgenic mice, basal and gonadotropin-releasing hormone (GnRH)-stimulated luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release from incubated pituitaries, as well as pituitary content of LH, FSH, and mRNA for their respective beta subunits, were measured in normal and transgenic males that were injected with testosterone propionate (5 micrograms/g body weight; 24 hours before autopsy), injected with oil vehicle, castrated for 10 days, or sham operated. In normal (non-transgenic) males, exogenous testosterone induced the expected suppression, and castration induced the expected stimulation of various parameters of gonadotropin synthesis and release. In contrast, in testosterone-treated and in castrated MT/hGH transgenic mice the release of LH and the pituitary levels of LH-beta mRNA did not differ from the corresponding values measured in vehicle-injected and sham-operated transgenic controls. Pituitary LH content was elevated in testosterone-treated MT/hGH transgenic mice but was not changed in castrated transgenic males. The changes in pituitary levels of FSH and FSH-beta mRNA and in FSH release in MT/hGH transgenic mice in response to testosterone and castration were different from the changes in LH and LH-beta mRNA in the same mice, but similar to the changes of FSH and FSH-beta message produced in normal mice by identical treatments. We suggest that hGH expression attenuates the effects of testosterone on the mechanisms controlling LH release, with less influence on testosterone regulation of LH synthesis. These effects of hGH expression appear to be selective for LH, without influencing the FSH control system.

Authors+Show Affiliations

Department of Physiology, School of Medicine, Southern Illinois University, Carbondale.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8188541

Citation

Tang, K, et al. "Testosterone Feedback On Gonadotropin Secretion and Gene Expression in Transgenic Mice Expressing Human Growth Hormone Gene." Journal of Andrology, vol. 15, no. 1, 1994, pp. 9-14.
Tang K, Bartke A, Gardiner CS, et al. Testosterone feedback on gonadotropin secretion and gene expression in transgenic mice expressing human growth hormone gene. J Androl. 1994;15(1):9-14.
Tang, K., Bartke, A., Gardiner, C. S., Wagner, T. E., & Yun, J. S. (1994). Testosterone feedback on gonadotropin secretion and gene expression in transgenic mice expressing human growth hormone gene. Journal of Andrology, 15(1), 9-14.
Tang K, et al. Testosterone Feedback On Gonadotropin Secretion and Gene Expression in Transgenic Mice Expressing Human Growth Hormone Gene. J Androl. 1994 Jan-Feb;15(1):9-14. PubMed PMID: 8188541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Testosterone feedback on gonadotropin secretion and gene expression in transgenic mice expressing human growth hormone gene. AU - Tang,K, AU - Bartke,A, AU - Gardiner,C S, AU - Wagner,T E, AU - Yun,J S, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 9 EP - 14 JF - Journal of andrology JO - J Androl VL - 15 IS - 1 N2 - To determine the effects of testosterone on the regulation of gonadotropins in metallothionein-1/human growth hormone (MT/hGH) transgenic mice, basal and gonadotropin-releasing hormone (GnRH)-stimulated luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release from incubated pituitaries, as well as pituitary content of LH, FSH, and mRNA for their respective beta subunits, were measured in normal and transgenic males that were injected with testosterone propionate (5 micrograms/g body weight; 24 hours before autopsy), injected with oil vehicle, castrated for 10 days, or sham operated. In normal (non-transgenic) males, exogenous testosterone induced the expected suppression, and castration induced the expected stimulation of various parameters of gonadotropin synthesis and release. In contrast, in testosterone-treated and in castrated MT/hGH transgenic mice the release of LH and the pituitary levels of LH-beta mRNA did not differ from the corresponding values measured in vehicle-injected and sham-operated transgenic controls. Pituitary LH content was elevated in testosterone-treated MT/hGH transgenic mice but was not changed in castrated transgenic males. The changes in pituitary levels of FSH and FSH-beta mRNA and in FSH release in MT/hGH transgenic mice in response to testosterone and castration were different from the changes in LH and LH-beta mRNA in the same mice, but similar to the changes of FSH and FSH-beta message produced in normal mice by identical treatments. We suggest that hGH expression attenuates the effects of testosterone on the mechanisms controlling LH release, with less influence on testosterone regulation of LH synthesis. These effects of hGH expression appear to be selective for LH, without influencing the FSH control system. SN - 0196-3635 UR - https://www.unboundmedicine.com/medline/citation/8188541/Testosterone_feedback_on_gonadotropin_secretion_and_gene_expression_in_transgenic_mice_expressing_human_growth_hormone_gene_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0196-3635&date=1994&volume=15&issue=1&spage=9 DB - PRIME DP - Unbound Medicine ER -