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The B-cell adhesion molecule CD22 is cross-species reactive and recognizes distinct sialoglycoproteins on different functional T-cell sub-populations.
Scand J Immunol. 1994 May; 39(5):433-8.SJ

Abstract

CD22 is a B cell lineage restricted cell-surface adhesion glycoprotein which recognizes ligands on human T and B cells and cell lines. A soluble recombinant form of human CD22 (hCD22Rg) has been used to identify and characterize CD22-specific ligands on human T cells, one of which has been shown to be the receptor-linked phosphotyrosine phosphatase CD45. Because CD45 plays a pivotal role in lymphocyte activation, we assessed whether human CD22 might display cross-species reactivity with CD45. In the study presented here we demonstrate that human CD22Rg recognizes several murine cell-surface sialoglycoproteins, including CD45, containing sialic acid in alpha 2,6 linkage. Furthermore, hCD22Rg recognizes different ligands on functionally distinct T helper-cell subpopulations and selectively binds medullary thymocytes in vivo. Our results confirm and extend previous observations that CD22 is a sialic acid-binding lectin which interacts with CD45 and other glycoproteins capable of presenting alpha 2,6-linked sialic acid in a manner that promotes high affinity binding. The cross-species reactivity of CD22 with its ligands underscores the potential physiologic importance of CD22-mediated lymphocyte interactions.

Authors+Show Affiliations

Department of Pathology, Massachusetts General Hospital, Charlestown.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8191218

Citation

Sgroi, D, and I Stamenkovic. "The B-cell Adhesion Molecule CD22 Is Cross-species Reactive and Recognizes Distinct Sialoglycoproteins On Different Functional T-cell Sub-populations." Scandinavian Journal of Immunology, vol. 39, no. 5, 1994, pp. 433-8.
Sgroi D, Stamenkovic I. The B-cell adhesion molecule CD22 is cross-species reactive and recognizes distinct sialoglycoproteins on different functional T-cell sub-populations. Scand J Immunol. 1994;39(5):433-8.
Sgroi, D., & Stamenkovic, I. (1994). The B-cell adhesion molecule CD22 is cross-species reactive and recognizes distinct sialoglycoproteins on different functional T-cell sub-populations. Scandinavian Journal of Immunology, 39(5), 433-8.
Sgroi D, Stamenkovic I. The B-cell Adhesion Molecule CD22 Is Cross-species Reactive and Recognizes Distinct Sialoglycoproteins On Different Functional T-cell Sub-populations. Scand J Immunol. 1994;39(5):433-8. PubMed PMID: 8191218.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The B-cell adhesion molecule CD22 is cross-species reactive and recognizes distinct sialoglycoproteins on different functional T-cell sub-populations. AU - Sgroi,D, AU - Stamenkovic,I, PY - 1994/5/1/pubmed PY - 1994/5/1/medline PY - 1994/5/1/entrez SP - 433 EP - 8 JF - Scandinavian journal of immunology JO - Scand J Immunol VL - 39 IS - 5 N2 - CD22 is a B cell lineage restricted cell-surface adhesion glycoprotein which recognizes ligands on human T and B cells and cell lines. A soluble recombinant form of human CD22 (hCD22Rg) has been used to identify and characterize CD22-specific ligands on human T cells, one of which has been shown to be the receptor-linked phosphotyrosine phosphatase CD45. Because CD45 plays a pivotal role in lymphocyte activation, we assessed whether human CD22 might display cross-species reactivity with CD45. In the study presented here we demonstrate that human CD22Rg recognizes several murine cell-surface sialoglycoproteins, including CD45, containing sialic acid in alpha 2,6 linkage. Furthermore, hCD22Rg recognizes different ligands on functionally distinct T helper-cell subpopulations and selectively binds medullary thymocytes in vivo. Our results confirm and extend previous observations that CD22 is a sialic acid-binding lectin which interacts with CD45 and other glycoproteins capable of presenting alpha 2,6-linked sialic acid in a manner that promotes high affinity binding. The cross-species reactivity of CD22 with its ligands underscores the potential physiologic importance of CD22-mediated lymphocyte interactions. SN - 0300-9475 UR - https://www.unboundmedicine.com/medline/citation/8191218/The_B_cell_adhesion_molecule_CD22_is_cross_species_reactive_and_recognizes_distinct_sialoglycoproteins_on_different_functional_T_cell_sub_populations_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0300-9475&date=1994&volume=39&issue=5&spage=433 DB - PRIME DP - Unbound Medicine ER -