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Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase.
J Bacteriol. 1994 Jun; 176(11):3420-7.JB

Abstract

A plasmid library of Salmonella typhimurium was used to complement a temperature-sensitive nrdA mutant of Escherichia coli. Complementation was obtained with two different classes of plasmids, one carrying the E. coli nrdAB-like genes and the second containing an operon encoding a new bacterial ribonucleotide reductase. Plasmids harboring these new reductase genes also enable obligately anaerobic nrdB::Mud1 E. coli mutants to grow in the presence of oxygen. This operon consists of two open reading frames, which have been designated nrdE (2,145 bp) and nrdF (969 bp). The deduced amino acid sequences of the nrdE and nrdF products include the catalytically important residues conserved in ribonucleotide reductase enzymes of class I and show 25 and 28% overall identity with the R1 and R2 protein, respectively, of the aerobic ribonucleoside diphosphate reductase of E. coli. The 3' end of the sequenced 4.9-kb fragment corresponds to the upstream region of the previously published proU operon of both S. typhimurium and E. coli, indicating that the nrdEF genes are at 57 min on the chromosomal maps of these two bacterial species. Analysis of the nrdEF and proU sequences demonstrates that transcription of the nrdEF genes is in the clockwise direction on the S. typhimurium and E. coli maps.

Authors+Show Affiliations

Department of Genetics and Microbiology, Faculty of Sciences, Autonomous University of Barcelona, Bellatera, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8195103

Citation

Jordan, A, et al. "Cloning and Sequencing of the Genes From Salmonella Typhimurium Encoding a New Bacterial Ribonucleotide Reductase." Journal of Bacteriology, vol. 176, no. 11, 1994, pp. 3420-7.
Jordan A, Gibert I, Barbé J. Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase. J Bacteriol. 1994;176(11):3420-7.
Jordan, A., Gibert, I., & Barbé, J. (1994). Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase. Journal of Bacteriology, 176(11), 3420-7.
Jordan A, Gibert I, Barbé J. Cloning and Sequencing of the Genes From Salmonella Typhimurium Encoding a New Bacterial Ribonucleotide Reductase. J Bacteriol. 1994;176(11):3420-7. PubMed PMID: 8195103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and sequencing of the genes from Salmonella typhimurium encoding a new bacterial ribonucleotide reductase. AU - Jordan,A, AU - Gibert,I, AU - Barbé,J, PY - 1994/6/1/pubmed PY - 1994/6/1/medline PY - 1994/6/1/entrez SP - 3420 EP - 7 JF - Journal of bacteriology JO - J Bacteriol VL - 176 IS - 11 N2 - A plasmid library of Salmonella typhimurium was used to complement a temperature-sensitive nrdA mutant of Escherichia coli. Complementation was obtained with two different classes of plasmids, one carrying the E. coli nrdAB-like genes and the second containing an operon encoding a new bacterial ribonucleotide reductase. Plasmids harboring these new reductase genes also enable obligately anaerobic nrdB::Mud1 E. coli mutants to grow in the presence of oxygen. This operon consists of two open reading frames, which have been designated nrdE (2,145 bp) and nrdF (969 bp). The deduced amino acid sequences of the nrdE and nrdF products include the catalytically important residues conserved in ribonucleotide reductase enzymes of class I and show 25 and 28% overall identity with the R1 and R2 protein, respectively, of the aerobic ribonucleoside diphosphate reductase of E. coli. The 3' end of the sequenced 4.9-kb fragment corresponds to the upstream region of the previously published proU operon of both S. typhimurium and E. coli, indicating that the nrdEF genes are at 57 min on the chromosomal maps of these two bacterial species. Analysis of the nrdEF and proU sequences demonstrates that transcription of the nrdEF genes is in the clockwise direction on the S. typhimurium and E. coli maps. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/8195103/Cloning_and_sequencing_of_the_genes_from_Salmonella_typhimurium_encoding_a_new_bacterial_ribonucleotide_reductase_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=8195103 DB - PRIME DP - Unbound Medicine ER -