Tags

Type your tag names separated by a space and hit enter

Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains.
Mol Gen Genet. 1994 May 25; 243(4):374-8.MG

Abstract

The stringent response causes inhibition of replication of plasmid pBR322 in amino acid-starved Escherichia coli cells whereas in relaxed mutants the replication of this plasmid proceeds for several hours. On the basis of density shift experiments and pulse-labelling experiments we showed that most of the pBR322 molecules begin replication during the relaxed response and the rate of plasmid DNA synthesis in unstarved and isoleucine-starved relA- bacteria is similar. We found that the Rom function plays a key role in the stringent control of plasmid pBR322 replication, as insertional inactivation of the rom gene causes amplification of pBR322rom- in both relA- and relA+ strains during amino acid starvation. Moreover, pUC19, which is a pBR322-derived plasmid lacking the rom gene, behaves like pBR322rom-, whereas introduction of the rom gene into the pUC19 replicon drives it into the pBR322 mode of replication in amino acid-starved bacteria. A model for the regulation of pBR322 plasmid DNA replication by Rom protein in amino acid-starved Escherichia coli strains is proposed.

Authors+Show Affiliations

Department of Molecular Biology, University of Gdańsk, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8202082

Citation

Herman, A, et al. "Regulation of Replication of Plasmid pBR322 in Amino Acid-starved Escherichia Coli Strains." Molecular & General Genetics : MGG, vol. 243, no. 4, 1994, pp. 374-8.
Herman A, Wegrzyn A, Wegrzyn G. Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains. Mol Gen Genet. 1994;243(4):374-8.
Herman, A., Wegrzyn, A., & Wegrzyn, G. (1994). Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains. Molecular & General Genetics : MGG, 243(4), 374-8.
Herman A, Wegrzyn A, Wegrzyn G. Regulation of Replication of Plasmid pBR322 in Amino Acid-starved Escherichia Coli Strains. Mol Gen Genet. 1994 May 25;243(4):374-8. PubMed PMID: 8202082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of replication of plasmid pBR322 in amino acid-starved Escherichia coli strains. AU - Herman,A, AU - Wegrzyn,A, AU - Wegrzyn,G, PY - 1994/5/25/pubmed PY - 1994/5/25/medline PY - 1994/5/25/entrez SP - 374 EP - 8 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 243 IS - 4 N2 - The stringent response causes inhibition of replication of plasmid pBR322 in amino acid-starved Escherichia coli cells whereas in relaxed mutants the replication of this plasmid proceeds for several hours. On the basis of density shift experiments and pulse-labelling experiments we showed that most of the pBR322 molecules begin replication during the relaxed response and the rate of plasmid DNA synthesis in unstarved and isoleucine-starved relA- bacteria is similar. We found that the Rom function plays a key role in the stringent control of plasmid pBR322 replication, as insertional inactivation of the rom gene causes amplification of pBR322rom- in both relA- and relA+ strains during amino acid starvation. Moreover, pUC19, which is a pBR322-derived plasmid lacking the rom gene, behaves like pBR322rom-, whereas introduction of the rom gene into the pUC19 replicon drives it into the pBR322 mode of replication in amino acid-starved bacteria. A model for the regulation of pBR322 plasmid DNA replication by Rom protein in amino acid-starved Escherichia coli strains is proposed. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/8202082/Regulation_of_replication_of_plasmid_pBR322_in_amino_acid_starved_Escherichia_coli_strains_ DB - PRIME DP - Unbound Medicine ER -