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Cytochemical study of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase activity in guinea pig facial nerve.
ORL J Otorhinolaryngol Relat Spec. 1994 May-Jun; 56(3):143-5.OJ

Abstract

The localization of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase (K-NPPase) activity, the second dephosphorylative property of the Na-K adenosine triphosphatase complex, was cytochemically studied in the intra-temporal portion of the facial nerve in normal guinea pigs using a cerium-based method. A fine-granular reaction product of the K-NPPase activity was observed on the cytoplasmic side of the axolemma of the axon cylinder, of the incisures of Schmidt-Lanterman, and of the nodes of Ranvier. No reaction product was detected on the periaxonal and outermost plasma membrane of Schwann cells and in the myelin sheath. In control tissue samples, the enzyme activity was almost completely inhibited by 10 mM ouabain, and no reaction was noted in medium without K+.

Authors+Show Affiliations

Department of Otolaryngology, Hyogo College of Medicine, Japan.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8202312

Citation

Kanoh, N, and T Kumoi. "Cytochemical Study of Ouabain-sensitive, K(+)-dependent P-nitrophenylphosphatase Activity in Guinea Pig Facial Nerve." ORL; Journal for Oto-rhino-laryngology and Its Related Specialties, vol. 56, no. 3, 1994, pp. 143-5.
Kanoh N, Kumoi T. Cytochemical study of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase activity in guinea pig facial nerve. ORL J Otorhinolaryngol Relat Spec. 1994;56(3):143-5.
Kanoh, N., & Kumoi, T. (1994). Cytochemical study of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase activity in guinea pig facial nerve. ORL; Journal for Oto-rhino-laryngology and Its Related Specialties, 56(3), 143-5.
Kanoh N, Kumoi T. Cytochemical Study of Ouabain-sensitive, K(+)-dependent P-nitrophenylphosphatase Activity in Guinea Pig Facial Nerve. ORL J Otorhinolaryngol Relat Spec. 1994 May-Jun;56(3):143-5. PubMed PMID: 8202312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochemical study of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase activity in guinea pig facial nerve. AU - Kanoh,N, AU - Kumoi,T, PY - 1994/5/1/pubmed PY - 1994/5/1/medline PY - 1994/5/1/entrez SP - 143 EP - 5 JF - ORL; journal for oto-rhino-laryngology and its related specialties JO - ORL J Otorhinolaryngol Relat Spec VL - 56 IS - 3 N2 - The localization of ouabain-sensitive, K(+)-dependent p-nitrophenylphosphatase (K-NPPase) activity, the second dephosphorylative property of the Na-K adenosine triphosphatase complex, was cytochemically studied in the intra-temporal portion of the facial nerve in normal guinea pigs using a cerium-based method. A fine-granular reaction product of the K-NPPase activity was observed on the cytoplasmic side of the axolemma of the axon cylinder, of the incisures of Schmidt-Lanterman, and of the nodes of Ranvier. No reaction product was detected on the periaxonal and outermost plasma membrane of Schwann cells and in the myelin sheath. In control tissue samples, the enzyme activity was almost completely inhibited by 10 mM ouabain, and no reaction was noted in medium without K+. SN - 0301-1569 UR - https://www.unboundmedicine.com/medline/citation/8202312/Cytochemical_study_of_ouabain_sensitive_K_+__dependent_p_nitrophenylphosphatase_activity_in_guinea_pig_facial_nerve_ L2 - https://www.karger.com?DOI=10.1159/000276630 DB - PRIME DP - Unbound Medicine ER -