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Transcriptional regulation of CD6 expression on human T lymphocytes by phorbol ester.
J Immunol. 1994 Jul 01; 153(1):1-9.JI

Abstract

Activation of protein kinase C (PKC) has emerged as a major common signal-transducing mechanism for T cell activation and regulation of expression of T cell surface glycoproteins. Surface expression of the CD6 Ag is known to increase with T cell activation and CD6 itself may be involved in regulation of T cell activation. Therefore, we performed experiments to determine whether activation of PKC by phorbol ester induced an increase in CD6 expression and to investigate the mechanisms of such an effect. CD6 surface expression was up-regulated substantially in response to PMA on both mature and immature T cells, but only negligibly on B cell lines. This increase was blocked by PKC inhibitors. The PMA-induced increase in CD6 surface expression was accompanied by an increase in total CD6 protein, as detected by using Western blot analysis of whole cell lysates. After PMA stimulation, Northern blot analysis showed that steady state levels of CD6 mRNA increased in response to PMA treatment. This increase was blocked by cycloheximide, demonstrating that it was dependent on new protein synthesis. Nuclear run-on analysis showed that the rate of CD6 mRNA transcription increased by approximately two to threefold after PMA stimulation of Jurkat cells. Experiments in which we used actinomycin D showed that PMA had no significant effect on the t1/2 of CD6 mRNA. The data suggest that the effect of PMA on CD6 expression is mediated primarily by an increase in CD6 mRNA transcription after PKC activation. mAbs were used to determine whether augmented CD6 expression could be induced by perturbation of specific T cell surface molecules. Up-regulation of CD6 expression occurred when thymocytes were cultured with anti-CD2 Abs, but not with Abs to other functional T cell surface structures, and not when mature T cells were cultured with the anti-CD2 mAbs. Up-regulation of CD6 expression by activation of PKC, triggered in thymocytes by ligation of CD2, could allow CD6 to provide additional regulatory signals required for events in later stages of T cell activation and differentiation.

Authors+Show Affiliations

Rackham Arthritis Research Unit, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8207228

Citation

Bott, C M., et al. "Transcriptional Regulation of CD6 Expression On Human T Lymphocytes By Phorbol Ester." Journal of Immunology (Baltimore, Md. : 1950), vol. 153, no. 1, 1994, pp. 1-9.
Bott CM, Doshi JB, Li LL, et al. Transcriptional regulation of CD6 expression on human T lymphocytes by phorbol ester. J Immunol. 1994;153(1):1-9.
Bott, C. M., Doshi, J. B., Li, L. L., McMurtry, S. A., Sanders, J. L., & Fox, D. A. (1994). Transcriptional regulation of CD6 expression on human T lymphocytes by phorbol ester. Journal of Immunology (Baltimore, Md. : 1950), 153(1), 1-9.
Bott CM, et al. Transcriptional Regulation of CD6 Expression On Human T Lymphocytes By Phorbol Ester. J Immunol. 1994 Jul 1;153(1):1-9. PubMed PMID: 8207228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptional regulation of CD6 expression on human T lymphocytes by phorbol ester. AU - Bott,C M, AU - Doshi,J B, AU - Li,L L, AU - McMurtry,S A, AU - Sanders,J L, AU - Fox,D A, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 1 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 153 IS - 1 N2 - Activation of protein kinase C (PKC) has emerged as a major common signal-transducing mechanism for T cell activation and regulation of expression of T cell surface glycoproteins. Surface expression of the CD6 Ag is known to increase with T cell activation and CD6 itself may be involved in regulation of T cell activation. Therefore, we performed experiments to determine whether activation of PKC by phorbol ester induced an increase in CD6 expression and to investigate the mechanisms of such an effect. CD6 surface expression was up-regulated substantially in response to PMA on both mature and immature T cells, but only negligibly on B cell lines. This increase was blocked by PKC inhibitors. The PMA-induced increase in CD6 surface expression was accompanied by an increase in total CD6 protein, as detected by using Western blot analysis of whole cell lysates. After PMA stimulation, Northern blot analysis showed that steady state levels of CD6 mRNA increased in response to PMA treatment. This increase was blocked by cycloheximide, demonstrating that it was dependent on new protein synthesis. Nuclear run-on analysis showed that the rate of CD6 mRNA transcription increased by approximately two to threefold after PMA stimulation of Jurkat cells. Experiments in which we used actinomycin D showed that PMA had no significant effect on the t1/2 of CD6 mRNA. The data suggest that the effect of PMA on CD6 expression is mediated primarily by an increase in CD6 mRNA transcription after PKC activation. mAbs were used to determine whether augmented CD6 expression could be induced by perturbation of specific T cell surface molecules. Up-regulation of CD6 expression occurred when thymocytes were cultured with anti-CD2 Abs, but not with Abs to other functional T cell surface structures, and not when mature T cells were cultured with the anti-CD2 mAbs. Up-regulation of CD6 expression by activation of PKC, triggered in thymocytes by ligation of CD2, could allow CD6 to provide additional regulatory signals required for events in later stages of T cell activation and differentiation. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8207228/Transcriptional_regulation_of_CD6_expression_on_human_T_lymphocytes_by_phorbol_ester_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=8207228 DB - PRIME DP - Unbound Medicine ER -