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Differential regulation of granulocyte-macrophage colony-stimulating factor gene expression in human corneal cells by pro-inflammatory cytokines.
J Immunol. 1994 Jul 01; 153(1):232-40.JI

Abstract

Neutrophils and Langerhans cells participate in inflammatory reactions within the human cornea. Because granulocyte-macrophage (GM)-CSF is a chemotactic and activating factor for these two cell types, we investigated whether this cytokine is produced by human corneal epithelial cells and corneal fibroblasts. Cultures of each cell type were exposed to increasing concentrations of IL-1 alpha or TNF-alpha. Culture supernatants were assayed for GM-CSF by using ELISA and cytokine mRNA levels were monitored by using reverse transcriptase-PCR. IL-1 alpha treatment of both cell types resulted in the appearance of GM-CSF mRNA and the production of > 480 pg protein/10(6) cells. However, TNF-alpha treatment yielded divergent results. Stimulation of epithelial cells with TNF-alpha resulted in the appearance of > 560 GM-CSF mRNA molecules per cell and production of > 1300 pg GM-CSF/10(6) cells. In contrast, stimulation of corneal fibroblasts resulted in < 16 GM-CSF mRNA molecules/cell and < 60 pg GM-CSF/10(6) cells. Binding studies with 125I-labeled TNF-alpha revealed that corneal fibroblasts had as many receptor sites as did corneal epithelial cells. Furthermore, corneal fibroblasts could respond to TNF-alpha-receptor-mediated signal transduction because they produced nanogram amounts of IL-6 after being treated with this cytokine. The results suggest that both cell types synthesize GM-CSF in response to IL-1 alpha, but that only corneal epithelial cells produce significant amounts of GM-CSF after TNF-alpha exposure. Differences in the responses of the two cell types to TNF-alpha may reflect a means of limiting accumulation of neutrophils and Langerhans cells and, thus, minimize corneal damage.

Authors+Show Affiliations

Department of Microbiology and Immunology, College of Medicine, University of South Alabama, Mobile 36688-0002.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8207239

Citation

Cubitt, C L., et al. "Differential Regulation of Granulocyte-macrophage Colony-stimulating Factor Gene Expression in Human Corneal Cells By Pro-inflammatory Cytokines." Journal of Immunology (Baltimore, Md. : 1950), vol. 153, no. 1, 1994, pp. 232-40.
Cubitt CL, Lausch RN, Oakes JE. Differential regulation of granulocyte-macrophage colony-stimulating factor gene expression in human corneal cells by pro-inflammatory cytokines. J Immunol. 1994;153(1):232-40.
Cubitt, C. L., Lausch, R. N., & Oakes, J. E. (1994). Differential regulation of granulocyte-macrophage colony-stimulating factor gene expression in human corneal cells by pro-inflammatory cytokines. Journal of Immunology (Baltimore, Md. : 1950), 153(1), 232-40.
Cubitt CL, Lausch RN, Oakes JE. Differential Regulation of Granulocyte-macrophage Colony-stimulating Factor Gene Expression in Human Corneal Cells By Pro-inflammatory Cytokines. J Immunol. 1994 Jul 1;153(1):232-40. PubMed PMID: 8207239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential regulation of granulocyte-macrophage colony-stimulating factor gene expression in human corneal cells by pro-inflammatory cytokines. AU - Cubitt,C L, AU - Lausch,R N, AU - Oakes,J E, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 232 EP - 40 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 153 IS - 1 N2 - Neutrophils and Langerhans cells participate in inflammatory reactions within the human cornea. Because granulocyte-macrophage (GM)-CSF is a chemotactic and activating factor for these two cell types, we investigated whether this cytokine is produced by human corneal epithelial cells and corneal fibroblasts. Cultures of each cell type were exposed to increasing concentrations of IL-1 alpha or TNF-alpha. Culture supernatants were assayed for GM-CSF by using ELISA and cytokine mRNA levels were monitored by using reverse transcriptase-PCR. IL-1 alpha treatment of both cell types resulted in the appearance of GM-CSF mRNA and the production of > 480 pg protein/10(6) cells. However, TNF-alpha treatment yielded divergent results. Stimulation of epithelial cells with TNF-alpha resulted in the appearance of > 560 GM-CSF mRNA molecules per cell and production of > 1300 pg GM-CSF/10(6) cells. In contrast, stimulation of corneal fibroblasts resulted in < 16 GM-CSF mRNA molecules/cell and < 60 pg GM-CSF/10(6) cells. Binding studies with 125I-labeled TNF-alpha revealed that corneal fibroblasts had as many receptor sites as did corneal epithelial cells. Furthermore, corneal fibroblasts could respond to TNF-alpha-receptor-mediated signal transduction because they produced nanogram amounts of IL-6 after being treated with this cytokine. The results suggest that both cell types synthesize GM-CSF in response to IL-1 alpha, but that only corneal epithelial cells produce significant amounts of GM-CSF after TNF-alpha exposure. Differences in the responses of the two cell types to TNF-alpha may reflect a means of limiting accumulation of neutrophils and Langerhans cells and, thus, minimize corneal damage. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8207239/Differential_regulation_of_granulocyte_macrophage_colony_stimulating_factor_gene_expression_in_human_corneal_cells_by_pro_inflammatory_cytokines_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&amp;pmid=8207239 DB - PRIME DP - Unbound Medicine ER -