Tags

Type your tag names separated by a space and hit enter

Functional platelet-activating factor receptors are expressed by monocytes and granulocytes but not by resting or activated T and B lymphocytes from normal individuals or patients with asthma.
J Immunol. 1994 Jul 01; 153(1):364-77.JI

Abstract

Platelet-activating factor (PAF) may play a role in the regulation of immune responsiveness and is a potent mediator in asthmatic inflammation. However, as yet, the mechanisms whereby PAF mediates its pleiomorphic effects on immune cells have not been elucidated. Because PAF is a potent chemotactic factor for eosinophils, the presence of receptors for PAF (PAFR) on lymphocytes may provide a mechanism for the concurrent recruitment of both eosinophils and T lymphocytes into the airways of asthmatic patients. To address this issue, we have examined freshly isolated PBMC and granulocytic cells as well as various T and B lymphocyte lines with regards to PAFR expression and PAF-induced changes in intracellular calcium concentration. Using two-color immunofluorescence techniques and highly purified cell populations, it was not possible to detect surface PAFR protein or functional PAFR on resting and in vivo or in vitro activated T and B cells derived from nonallergic individuals or patients with allergic asthma. In addition, we were unable to detect PAFR mRNA, protein, or functional response to PAF in human or murine T cell lines. In contrast, we found functional PAFR in most B lymphoblastoid cell lines. Within the PBMC population, CD14+ cells respond to PAF. These results suggest that PAF does not interact directly with lymphocytes and thus that previous observations suggestive of such an interaction likely reflect the effects of PAF on monocytes. PAF-induced increases in intracellular calcium concentration were also detected in neutrophils and eosinophils, but were lower in granulocytes relative to the levels detected in monocytes.

Authors+Show Affiliations

Swiss Institute of Allergy and Asthma Research, Davos.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8207248

Citation

Simon, H U., et al. "Functional Platelet-activating Factor Receptors Are Expressed By Monocytes and Granulocytes but Not By Resting or Activated T and B Lymphocytes From Normal Individuals or Patients With Asthma." Journal of Immunology (Baltimore, Md. : 1950), vol. 153, no. 1, 1994, pp. 364-77.
Simon HU, Tsao PW, Siminovitch KA, et al. Functional platelet-activating factor receptors are expressed by monocytes and granulocytes but not by resting or activated T and B lymphocytes from normal individuals or patients with asthma. J Immunol. 1994;153(1):364-77.
Simon, H. U., Tsao, P. W., Siminovitch, K. A., Mills, G. B., & Blaser, K. (1994). Functional platelet-activating factor receptors are expressed by monocytes and granulocytes but not by resting or activated T and B lymphocytes from normal individuals or patients with asthma. Journal of Immunology (Baltimore, Md. : 1950), 153(1), 364-77.
Simon HU, et al. Functional Platelet-activating Factor Receptors Are Expressed By Monocytes and Granulocytes but Not By Resting or Activated T and B Lymphocytes From Normal Individuals or Patients With Asthma. J Immunol. 1994 Jul 1;153(1):364-77. PubMed PMID: 8207248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional platelet-activating factor receptors are expressed by monocytes and granulocytes but not by resting or activated T and B lymphocytes from normal individuals or patients with asthma. AU - Simon,H U, AU - Tsao,P W, AU - Siminovitch,K A, AU - Mills,G B, AU - Blaser,K, PY - 1994/7/1/pubmed PY - 1994/7/1/medline PY - 1994/7/1/entrez SP - 364 EP - 77 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 153 IS - 1 N2 - Platelet-activating factor (PAF) may play a role in the regulation of immune responsiveness and is a potent mediator in asthmatic inflammation. However, as yet, the mechanisms whereby PAF mediates its pleiomorphic effects on immune cells have not been elucidated. Because PAF is a potent chemotactic factor for eosinophils, the presence of receptors for PAF (PAFR) on lymphocytes may provide a mechanism for the concurrent recruitment of both eosinophils and T lymphocytes into the airways of asthmatic patients. To address this issue, we have examined freshly isolated PBMC and granulocytic cells as well as various T and B lymphocyte lines with regards to PAFR expression and PAF-induced changes in intracellular calcium concentration. Using two-color immunofluorescence techniques and highly purified cell populations, it was not possible to detect surface PAFR protein or functional PAFR on resting and in vivo or in vitro activated T and B cells derived from nonallergic individuals or patients with allergic asthma. In addition, we were unable to detect PAFR mRNA, protein, or functional response to PAF in human or murine T cell lines. In contrast, we found functional PAFR in most B lymphoblastoid cell lines. Within the PBMC population, CD14+ cells respond to PAF. These results suggest that PAF does not interact directly with lymphocytes and thus that previous observations suggestive of such an interaction likely reflect the effects of PAF on monocytes. PAF-induced increases in intracellular calcium concentration were also detected in neutrophils and eosinophils, but were lower in granulocytes relative to the levels detected in monocytes. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8207248/Functional_platelet_activating_factor_receptors_are_expressed_by_monocytes_and_granulocytes_but_not_by_resting_or_activated_T_and_B_lymphocytes_from_normal_individuals_or_patients_with_asthma_ DB - PRIME DP - Unbound Medicine ER -