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Epstein-Barr virus-latent gene expression and tumor cell phenotype in acquired immunodeficiency syndrome-related non-Hodgkin's lymphoma. Correlation of lymphoma phenotype with three distinct patterns of viral latency.
Am J Pathol. 1993 Oct; 143(4):1072-85.AJ

Abstract

We investigated 49 acquired immunodeficiency syndrome-related lymphomas (ARLs) for Epstein-Barr virus (EBV) by Southern blotting and in situ hybridization and, in positive cases, used cryostat immunohistology to compare EBV-latent gene expression (EBV encoded small RNA-1 [EBER-1], EBV nuclear antigen-2 [EBNA-2], latent membrane protein-1 [LMP-1] and host cell immunophenotype (CD11a, CD18, CD54, CD58, CD21, CD23, CD30, CD39, CDw70, immunoglobulin) patterns with those reported in other EBV infections. EBV+ immunoblast-rich/large cell ARLs (n = 22) showed three patterns of latency: broad (EBER+EBNA-2+/LMP-1+; n = 9), reminiscent of a lymphoblastoid cell line phenotype; restricted (EBER+/EBNA-2-/LMP-1-; n = 6), similar to endemic Burkitt's lymphoma; and intermediate (EBER+/EBNA-2-/LMP-1+; n = 7), a pattern rarely described in vitro but seen in certain EBV-related malignancies. EBNA-2 expression was associated with extranodal lymphomas. EBV+ Burkitt-type ARLs (n = 11) usually showed the restricted latency pattern (n = 8), but some expressed the intermediate form (n = 3). Adhesion (CD54, CD58) and activation (CD30, CD39, CDw70) molecule expression varied with morphology (immunoblast-rich/large cell versus Burkitt-type), but was not independently correlated with EBV-positivity. CD30 and LMP-1 expression were associated. ARLs show heterogeneity regarding both the presence of EBV and latency pattern. Comparison of these phenotypically distinct lymphoma groups with known forms of EBV infection provides clues to their possible pathogenesis.

Authors+Show Affiliations

Laboratory of Immunohistology, Aarhus University Hospital, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8214003

Citation

Hamilton-Dutoit, S J., et al. "Epstein-Barr Virus-latent Gene Expression and Tumor Cell Phenotype in Acquired Immunodeficiency Syndrome-related non-Hodgkin's Lymphoma. Correlation of Lymphoma Phenotype With Three Distinct Patterns of Viral Latency." The American Journal of Pathology, vol. 143, no. 4, 1993, pp. 1072-85.
Hamilton-Dutoit SJ, Rea D, Raphael M, et al. Epstein-Barr virus-latent gene expression and tumor cell phenotype in acquired immunodeficiency syndrome-related non-Hodgkin's lymphoma. Correlation of lymphoma phenotype with three distinct patterns of viral latency. Am J Pathol. 1993;143(4):1072-85.
Hamilton-Dutoit, S. J., Rea, D., Raphael, M., Sandvej, K., Delecluse, H. J., Gisselbrecht, C., Marelle, L., van Krieken, H. J., & Pallesen, G. (1993). Epstein-Barr virus-latent gene expression and tumor cell phenotype in acquired immunodeficiency syndrome-related non-Hodgkin's lymphoma. Correlation of lymphoma phenotype with three distinct patterns of viral latency. The American Journal of Pathology, 143(4), 1072-85.
Hamilton-Dutoit SJ, et al. Epstein-Barr Virus-latent Gene Expression and Tumor Cell Phenotype in Acquired Immunodeficiency Syndrome-related non-Hodgkin's Lymphoma. Correlation of Lymphoma Phenotype With Three Distinct Patterns of Viral Latency. Am J Pathol. 1993;143(4):1072-85. PubMed PMID: 8214003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus-latent gene expression and tumor cell phenotype in acquired immunodeficiency syndrome-related non-Hodgkin's lymphoma. Correlation of lymphoma phenotype with three distinct patterns of viral latency. AU - Hamilton-Dutoit,S J, AU - Rea,D, AU - Raphael,M, AU - Sandvej,K, AU - Delecluse,H J, AU - Gisselbrecht,C, AU - Marelle,L, AU - van Krieken,H J, AU - Pallesen,G, PY - 1993/10/1/pubmed PY - 1993/10/1/medline PY - 1993/10/1/entrez SP - 1072 EP - 85 JF - The American journal of pathology JO - Am. J. Pathol. VL - 143 IS - 4 N2 - We investigated 49 acquired immunodeficiency syndrome-related lymphomas (ARLs) for Epstein-Barr virus (EBV) by Southern blotting and in situ hybridization and, in positive cases, used cryostat immunohistology to compare EBV-latent gene expression (EBV encoded small RNA-1 [EBER-1], EBV nuclear antigen-2 [EBNA-2], latent membrane protein-1 [LMP-1] and host cell immunophenotype (CD11a, CD18, CD54, CD58, CD21, CD23, CD30, CD39, CDw70, immunoglobulin) patterns with those reported in other EBV infections. EBV+ immunoblast-rich/large cell ARLs (n = 22) showed three patterns of latency: broad (EBER+EBNA-2+/LMP-1+; n = 9), reminiscent of a lymphoblastoid cell line phenotype; restricted (EBER+/EBNA-2-/LMP-1-; n = 6), similar to endemic Burkitt's lymphoma; and intermediate (EBER+/EBNA-2-/LMP-1+; n = 7), a pattern rarely described in vitro but seen in certain EBV-related malignancies. EBNA-2 expression was associated with extranodal lymphomas. EBV+ Burkitt-type ARLs (n = 11) usually showed the restricted latency pattern (n = 8), but some expressed the intermediate form (n = 3). Adhesion (CD54, CD58) and activation (CD30, CD39, CDw70) molecule expression varied with morphology (immunoblast-rich/large cell versus Burkitt-type), but was not independently correlated with EBV-positivity. CD30 and LMP-1 expression were associated. ARLs show heterogeneity regarding both the presence of EBV and latency pattern. Comparison of these phenotypically distinct lymphoma groups with known forms of EBV infection provides clues to their possible pathogenesis. SN - 0002-9440 UR - https://www.unboundmedicine.com/medline/citation/8214003/Epstein_Barr_virus_latent_gene_expression_and_tumor_cell_phenotype_in_acquired_immunodeficiency_syndrome_related_non_Hodgkin's_lymphoma__Correlation_of_lymphoma_phenotype_with_three_distinct_patterns_of_viral_latency_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/8214003/ DB - PRIME DP - Unbound Medicine ER -