Tags

Type your tag names separated by a space and hit enter

Formation of tight monolayers of guinea pig airway epithelial cells cultured in an air-interface: bioelectric properties.
Biotechniques 1993; 15(3):468-73B

Abstract

In this study, we have developed an air-interface culture system in which guinea pig tracheobronchial epithelial (GPTE) cells rapidly form tight monolayers. Enzymatically isolated GPTE cells were plated on collagen-treated polycarbonate microporous cell culture inserts at a density of 10(6) cells/cm2 (day 0). Bioelectric properties of cultures grown in an air-interface were compared with those covered by medium. On day 1 for air-interface cultures, apical fluid was removed and basolateral fluid was replenished. For cultures covered by medium, varying volumes of apical fluid were used. On days 4 and 5 after plating, confluent GPTE monolayers in either liquid-covered or air-interface cultures exhibited similar monolayer resistance values > or = 1.0 kohm-cm2. However, the equivalent short-circuit current (Ieq) was significantly higher in air-interface cultures than those covered with medium on days 4 and 5. The Ieq in air-interface cultures on day 4 was 12.9 microA/cm2. These confluent GPTE cell monolayers cultured in air-interface could be a useful tool for studies of changes in bioelectric and ion transport properties in response to injury and mediators of inflammation.

Authors+Show Affiliations

Childrens Hospital, Los Angeles, California.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8217160

Citation

Robison, T W., et al. "Formation of Tight Monolayers of Guinea Pig Airway Epithelial Cells Cultured in an Air-interface: Bioelectric Properties." BioTechniques, vol. 15, no. 3, 1993, pp. 468-73.
Robison TW, Dorio RJ, Kim KJ. Formation of tight monolayers of guinea pig airway epithelial cells cultured in an air-interface: bioelectric properties. BioTechniques. 1993;15(3):468-73.
Robison, T. W., Dorio, R. J., & Kim, K. J. (1993). Formation of tight monolayers of guinea pig airway epithelial cells cultured in an air-interface: bioelectric properties. BioTechniques, 15(3), pp. 468-73.
Robison TW, Dorio RJ, Kim KJ. Formation of Tight Monolayers of Guinea Pig Airway Epithelial Cells Cultured in an Air-interface: Bioelectric Properties. BioTechniques. 1993;15(3):468-73. PubMed PMID: 8217160.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Formation of tight monolayers of guinea pig airway epithelial cells cultured in an air-interface: bioelectric properties. AU - Robison,T W, AU - Dorio,R J, AU - Kim,K J, PY - 1993/9/1/pubmed PY - 1993/9/1/medline PY - 1993/9/1/entrez SP - 468 EP - 73 JF - BioTechniques JO - BioTechniques VL - 15 IS - 3 N2 - In this study, we have developed an air-interface culture system in which guinea pig tracheobronchial epithelial (GPTE) cells rapidly form tight monolayers. Enzymatically isolated GPTE cells were plated on collagen-treated polycarbonate microporous cell culture inserts at a density of 10(6) cells/cm2 (day 0). Bioelectric properties of cultures grown in an air-interface were compared with those covered by medium. On day 1 for air-interface cultures, apical fluid was removed and basolateral fluid was replenished. For cultures covered by medium, varying volumes of apical fluid were used. On days 4 and 5 after plating, confluent GPTE monolayers in either liquid-covered or air-interface cultures exhibited similar monolayer resistance values > or = 1.0 kohm-cm2. However, the equivalent short-circuit current (Ieq) was significantly higher in air-interface cultures than those covered with medium on days 4 and 5. The Ieq in air-interface cultures on day 4 was 12.9 microA/cm2. These confluent GPTE cell monolayers cultured in air-interface could be a useful tool for studies of changes in bioelectric and ion transport properties in response to injury and mediators of inflammation. SN - 0736-6205 UR - https://www.unboundmedicine.com/medline/citation/8217160/Formation_of_tight_monolayers_of_guinea_pig_airway_epithelial_cells_cultured_in_an_air_interface:_bioelectric_properties_ DB - PRIME DP - Unbound Medicine ER -