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Effects of choline-free plasma induced by choline oxidase on regional levels of choline and acetylcholine in rat brain.
Brain Res Bull. 1993; 32(6):593-9.BR

Abstract

Choline-free plasma (CFP) was induced in rats by intravenous (IV) injection of 56.0 x 10(2) units kg-1 of choline oxidase (ChO) which completely metabolized the free Ch circulating in the plasma for at least 15.0 h and caused subsequent significant decrease in the concentration of free Ch in the three brain regions examined, the striatum, hippocampus, and cortex. However, the treatment did not affect concentrations of acetylcholine (ACh) in these regions. By contrast, intraperitoneal (IP) injection of 1.0 mmol kg-1 Ch chloride resulted in a maximum concentration of free Ch in plasma in 5 min, after which tissue Ch in all regions examined increased (p < 0.001). Concomitant increases were observed in cortical and hippocampal ACh (p < 0.05) 20 min after the injection. It is thus suggested that the brain may possess compensative mechanisms to prevent the supply of free Ch from circulating to the brain during synthesis of ACh in the brain. It is also suggested that the CFP rat would be a useful and readily available animal model for future study.

Authors+Show Affiliations

Department of Neuropsychopharmacology (Tsumura), Gunma University, School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8221157

Citation

Ikarashi, Y, et al. "Effects of Choline-free Plasma Induced By Choline Oxidase On Regional Levels of Choline and Acetylcholine in Rat Brain." Brain Research Bulletin, vol. 32, no. 6, 1993, pp. 593-9.
Ikarashi Y, Takahashi A, Ishimaru H, et al. Effects of choline-free plasma induced by choline oxidase on regional levels of choline and acetylcholine in rat brain. Brain Res Bull. 1993;32(6):593-9.
Ikarashi, Y., Takahashi, A., Ishimaru, H., Arai, T., & Maruyama, Y. (1993). Effects of choline-free plasma induced by choline oxidase on regional levels of choline and acetylcholine in rat brain. Brain Research Bulletin, 32(6), 593-9.
Ikarashi Y, et al. Effects of Choline-free Plasma Induced By Choline Oxidase On Regional Levels of Choline and Acetylcholine in Rat Brain. Brain Res Bull. 1993;32(6):593-9. PubMed PMID: 8221157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of choline-free plasma induced by choline oxidase on regional levels of choline and acetylcholine in rat brain. AU - Ikarashi,Y, AU - Takahashi,A, AU - Ishimaru,H, AU - Arai,T, AU - Maruyama,Y, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 593 EP - 9 JF - Brain research bulletin JO - Brain Res Bull VL - 32 IS - 6 N2 - Choline-free plasma (CFP) was induced in rats by intravenous (IV) injection of 56.0 x 10(2) units kg-1 of choline oxidase (ChO) which completely metabolized the free Ch circulating in the plasma for at least 15.0 h and caused subsequent significant decrease in the concentration of free Ch in the three brain regions examined, the striatum, hippocampus, and cortex. However, the treatment did not affect concentrations of acetylcholine (ACh) in these regions. By contrast, intraperitoneal (IP) injection of 1.0 mmol kg-1 Ch chloride resulted in a maximum concentration of free Ch in plasma in 5 min, after which tissue Ch in all regions examined increased (p < 0.001). Concomitant increases were observed in cortical and hippocampal ACh (p < 0.05) 20 min after the injection. It is thus suggested that the brain may possess compensative mechanisms to prevent the supply of free Ch from circulating to the brain during synthesis of ACh in the brain. It is also suggested that the CFP rat would be a useful and readily available animal model for future study. SN - 0361-9230 UR - https://www.unboundmedicine.com/medline/citation/8221157/Effects_of_choline_free_plasma_induced_by_choline_oxidase_on_regional_levels_of_choline_and_acetylcholine_in_rat_brain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0361-9230(93)90160-D DB - PRIME DP - Unbound Medicine ER -