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Localization of the CHIP28 water channel in reabsorptive segments of the rat male reproductive tract.
Eur J Cell Biol. 1993 Aug; 61(2):264-73.EJ

Abstract

The water channel protein CHIP28 is responsible for the high constitutive plasma membrane permeability to water of erythrocytes, renal proximal tubule, and thin descending limb of Henle. The male reproductive tract is embryologically related to kidney and some segments, particularly the efferent ducts, exhibit a high rate of solute flux-dependent reabsorption of luminal fluid. To determine whether this could occur through water channels in the plasma membrane of reproductive tract epithelial cells, we used anti-CHIP28 antibodies to localize this protein by Western blotting and immunocytochemistry. Western blotting of proteins from efferent duct homogenate indicated the presence of CHIP28 in the efferent duct cells. By indirect immunofluorescence and protein A-gold immunolabeling, CHIP28 was localized to the brush-border and basolateral membranes of nonciliated cells. Ciliated cells in the same epithelium showed no plasma membrane staining for CHIP28. In accord with immunocytochemical findings, freeze-fracture of nonciliated efferent duct cells revealed a plasma membrane organization resembling that of renal proximal tubule cells that are rich in CHIP28. The anti-CHIP28 antibodies also stained plasma membranes of epithelial cells in the ampulla of the vas deferens, seminal vesicles, and prostate, but not the cells in seminiferous tubules, epididymis, and proximal parts of the vas deferens. Therefore, CHIP28 may be a principal mediator of the transmembrane water transport in absorptive epithelial cells of efferent ducts, as well as in epithelial of several other segments of the male reproductive tract that show both secretory and reabsorptive functions.

Authors+Show Affiliations

Renal Unit, Massachusetts General Hospital, Charlestown 02129.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8223717

Citation

Brown, D, et al. "Localization of the CHIP28 Water Channel in Reabsorptive Segments of the Rat Male Reproductive Tract." European Journal of Cell Biology, vol. 61, no. 2, 1993, pp. 264-73.
Brown D, Verbavatz JM, Valenti G, et al. Localization of the CHIP28 water channel in reabsorptive segments of the rat male reproductive tract. Eur J Cell Biol. 1993;61(2):264-73.
Brown, D., Verbavatz, J. M., Valenti, G., Lui, B., & Sabolić, I. (1993). Localization of the CHIP28 water channel in reabsorptive segments of the rat male reproductive tract. European Journal of Cell Biology, 61(2), 264-73.
Brown D, et al. Localization of the CHIP28 Water Channel in Reabsorptive Segments of the Rat Male Reproductive Tract. Eur J Cell Biol. 1993;61(2):264-73. PubMed PMID: 8223717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of the CHIP28 water channel in reabsorptive segments of the rat male reproductive tract. AU - Brown,D, AU - Verbavatz,J M, AU - Valenti,G, AU - Lui,B, AU - Sabolić,I, PY - 1993/8/1/pubmed PY - 1993/8/1/medline PY - 1993/8/1/entrez SP - 264 EP - 73 JF - European journal of cell biology JO - Eur J Cell Biol VL - 61 IS - 2 N2 - The water channel protein CHIP28 is responsible for the high constitutive plasma membrane permeability to water of erythrocytes, renal proximal tubule, and thin descending limb of Henle. The male reproductive tract is embryologically related to kidney and some segments, particularly the efferent ducts, exhibit a high rate of solute flux-dependent reabsorption of luminal fluid. To determine whether this could occur through water channels in the plasma membrane of reproductive tract epithelial cells, we used anti-CHIP28 antibodies to localize this protein by Western blotting and immunocytochemistry. Western blotting of proteins from efferent duct homogenate indicated the presence of CHIP28 in the efferent duct cells. By indirect immunofluorescence and protein A-gold immunolabeling, CHIP28 was localized to the brush-border and basolateral membranes of nonciliated cells. Ciliated cells in the same epithelium showed no plasma membrane staining for CHIP28. In accord with immunocytochemical findings, freeze-fracture of nonciliated efferent duct cells revealed a plasma membrane organization resembling that of renal proximal tubule cells that are rich in CHIP28. The anti-CHIP28 antibodies also stained plasma membranes of epithelial cells in the ampulla of the vas deferens, seminal vesicles, and prostate, but not the cells in seminiferous tubules, epididymis, and proximal parts of the vas deferens. Therefore, CHIP28 may be a principal mediator of the transmembrane water transport in absorptive epithelial cells of efferent ducts, as well as in epithelial of several other segments of the male reproductive tract that show both secretory and reabsorptive functions. SN - 0171-9335 UR - https://www.unboundmedicine.com/medline/citation/8223717/Localization_of_the_CHIP28_water_channel_in_reabsorptive_segments_of_the_rat_male_reproductive_tract_ DB - PRIME DP - Unbound Medicine ER -