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Cell substratum modulates responses of preosteoblasts to retinoic acid.
J Cell Physiol. 1993 Nov; 157(2):243-52.JC

Abstract

The aim of this study was to determine the role of ECM components of bone in regulating the differentiation and function of cells of the osteoblast lineage. Rat UMR 201 cells, phenotypically preosteoblast, were plated onto plastic tissue culture dishes or dishes coated with gelled type I collagen or reconstituted basement membrane (matrigel). Acute cell attachment assays showed that cells adhered to substrates in the following order: collagen > matrigel >> plastic. Proliferation rate up to 96 hr were similar on each substrate. However, if cells were treated with 10(-6) M retinoic acid (RA), proliferation rates were reduced compared with control for cells grown on collagen and matrigel but not on plastic. Morphological changes were matrix-specific; in subconfluent cultures, long thin processes were seen with cells grown on collagen and a pattern of interconnecting cell processes formed when cells were plated on matrigel. Striking differences were observed in the constitutive or RA-induced gene expression of cells grown on the different substrates. When cells plated on collagen were treated with RA, induction of mRNA for alkaline phosphatase (ALP) as well as ALP enzyme activity were much less than with cells grown on plastic. In contrast, RA treatment induced osteopontin (OP) mRNA expression more strongly in cells plated on collagen compared with plastic within 24 hr and this was maintained for 72 hr. RA treatment produced a two fold increase of pro-alpha 1(I) collagen mRNA in cells grown on plastic and matrigel but not in cells grown on collagen. Growth on collagen produced changes in the way UMR 201 cells responded to RA from which they did not fully recover in subsequent 48-hr growth periods on plastic. These results indicate that ECM components regulate the function of and are capable of modulating RA-induced differentiation of preosteoblasts.

Authors+Show Affiliations

Department of Medicine, St. Vincent's Hospital, University of Melbourne, Fitzroy, Victoria, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8227157

Citation

Traianedes, K, et al. "Cell Substratum Modulates Responses of Preosteoblasts to Retinoic Acid." Journal of Cellular Physiology, vol. 157, no. 2, 1993, pp. 243-52.
Traianedes K, Ng KW, Martin TJ, et al. Cell substratum modulates responses of preosteoblasts to retinoic acid. J Cell Physiol. 1993;157(2):243-52.
Traianedes, K., Ng, K. W., Martin, T. J., & Findlay, D. M. (1993). Cell substratum modulates responses of preosteoblasts to retinoic acid. Journal of Cellular Physiology, 157(2), 243-52.
Traianedes K, et al. Cell Substratum Modulates Responses of Preosteoblasts to Retinoic Acid. J Cell Physiol. 1993;157(2):243-52. PubMed PMID: 8227157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell substratum modulates responses of preosteoblasts to retinoic acid. AU - Traianedes,K, AU - Ng,K W, AU - Martin,T J, AU - Findlay,D M, PY - 1993/11/1/pubmed PY - 1993/11/1/medline PY - 1993/11/1/entrez SP - 243 EP - 52 JF - Journal of cellular physiology JO - J Cell Physiol VL - 157 IS - 2 N2 - The aim of this study was to determine the role of ECM components of bone in regulating the differentiation and function of cells of the osteoblast lineage. Rat UMR 201 cells, phenotypically preosteoblast, were plated onto plastic tissue culture dishes or dishes coated with gelled type I collagen or reconstituted basement membrane (matrigel). Acute cell attachment assays showed that cells adhered to substrates in the following order: collagen > matrigel >> plastic. Proliferation rate up to 96 hr were similar on each substrate. However, if cells were treated with 10(-6) M retinoic acid (RA), proliferation rates were reduced compared with control for cells grown on collagen and matrigel but not on plastic. Morphological changes were matrix-specific; in subconfluent cultures, long thin processes were seen with cells grown on collagen and a pattern of interconnecting cell processes formed when cells were plated on matrigel. Striking differences were observed in the constitutive or RA-induced gene expression of cells grown on the different substrates. When cells plated on collagen were treated with RA, induction of mRNA for alkaline phosphatase (ALP) as well as ALP enzyme activity were much less than with cells grown on plastic. In contrast, RA treatment induced osteopontin (OP) mRNA expression more strongly in cells plated on collagen compared with plastic within 24 hr and this was maintained for 72 hr. RA treatment produced a two fold increase of pro-alpha 1(I) collagen mRNA in cells grown on plastic and matrigel but not in cells grown on collagen. Growth on collagen produced changes in the way UMR 201 cells responded to RA from which they did not fully recover in subsequent 48-hr growth periods on plastic. These results indicate that ECM components regulate the function of and are capable of modulating RA-induced differentiation of preosteoblasts. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/8227157/Cell_substratum_modulates_responses_of_preosteoblasts_to_retinoic_acid_ L2 - https://doi.org/10.1002/jcp.1041570206 DB - PRIME DP - Unbound Medicine ER -