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cis-elements involved in alternative splicing in the rat beta-tropomyosin gene: the 3'-splice site of the skeletal muscle exon 7 is the major site of blockage in nonmuscle cells.
Nucleic Acids Res. 1993 Oct 11; 21(20):4762-8.NA

Abstract

We have been using the rat beta-tropomyosin (beta-TM) gene as a model system to study the mechanism of alternative splicing. The beta-TM gene spans 10 kb with 11 exons and encodes two distinct isoforms, namely skeletal muscle beta-TM and fibroblast TM-1. Exons 1-5, 8, and 9 are common to all mRNAs expressed from this gene. Exons 6 and 11 are used in fibroblasts, as well as in smooth muscle cells, whereas exons 7 and 10 are used exclusively in skeletal muscle cells. Our previous studies localized the critical elements for regulated alternative splicing to sequences within exon 7 and the adjacent upstream intron. We also demonstrated that these sequences function, in part, to regulate splice-site selection in vivo by interacting with cellular factors that block the use of the skeletal muscle exon in nonmuscle cells (1). Here we have further characterized the critical cis-acting elements involved in alternative splice site selection. Our data demonstrate that exon 7 and its flanking intron sequences are sufficient to regulate the suppression of exon 7 in nonmuscle cells when flanked by heterologous exons derived from adenovirus. We have also shown by both in vivo and in vitro assays that the blockage of exon 7 in nonmuscle cells is primarily at its 3'-splice site. A model is presented for regulated alternative splicing in both skeletal muscle and nonmuscle cells.

Authors+Show Affiliations

Cold Spring Harbor Laboratory, NY 11724.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8233825

Citation

Guo, W, and D M. Helfman. "Cis-elements Involved in Alternative Splicing in the Rat Beta-tropomyosin Gene: the 3'-splice Site of the Skeletal Muscle Exon 7 Is the Major Site of Blockage in Nonmuscle Cells." Nucleic Acids Research, vol. 21, no. 20, 1993, pp. 4762-8.
Guo W, Helfman DM. Cis-elements involved in alternative splicing in the rat beta-tropomyosin gene: the 3'-splice site of the skeletal muscle exon 7 is the major site of blockage in nonmuscle cells. Nucleic Acids Res. 1993;21(20):4762-8.
Guo, W., & Helfman, D. M. (1993). Cis-elements involved in alternative splicing in the rat beta-tropomyosin gene: the 3'-splice site of the skeletal muscle exon 7 is the major site of blockage in nonmuscle cells. Nucleic Acids Research, 21(20), 4762-8.
Guo W, Helfman DM. Cis-elements Involved in Alternative Splicing in the Rat Beta-tropomyosin Gene: the 3'-splice Site of the Skeletal Muscle Exon 7 Is the Major Site of Blockage in Nonmuscle Cells. Nucleic Acids Res. 1993 Oct 11;21(20):4762-8. PubMed PMID: 8233825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - cis-elements involved in alternative splicing in the rat beta-tropomyosin gene: the 3'-splice site of the skeletal muscle exon 7 is the major site of blockage in nonmuscle cells. AU - Guo,W, AU - Helfman,D M, PY - 1993/10/11/pubmed PY - 1993/10/11/medline PY - 1993/10/11/entrez SP - 4762 EP - 8 JF - Nucleic acids research JO - Nucleic Acids Res VL - 21 IS - 20 N2 - We have been using the rat beta-tropomyosin (beta-TM) gene as a model system to study the mechanism of alternative splicing. The beta-TM gene spans 10 kb with 11 exons and encodes two distinct isoforms, namely skeletal muscle beta-TM and fibroblast TM-1. Exons 1-5, 8, and 9 are common to all mRNAs expressed from this gene. Exons 6 and 11 are used in fibroblasts, as well as in smooth muscle cells, whereas exons 7 and 10 are used exclusively in skeletal muscle cells. Our previous studies localized the critical elements for regulated alternative splicing to sequences within exon 7 and the adjacent upstream intron. We also demonstrated that these sequences function, in part, to regulate splice-site selection in vivo by interacting with cellular factors that block the use of the skeletal muscle exon in nonmuscle cells (1). Here we have further characterized the critical cis-acting elements involved in alternative splice site selection. Our data demonstrate that exon 7 and its flanking intron sequences are sufficient to regulate the suppression of exon 7 in nonmuscle cells when flanked by heterologous exons derived from adenovirus. We have also shown by both in vivo and in vitro assays that the blockage of exon 7 in nonmuscle cells is primarily at its 3'-splice site. A model is presented for regulated alternative splicing in both skeletal muscle and nonmuscle cells. SN - 0305-1048 UR - https://www.unboundmedicine.com/medline/citation/8233825/cis_elements_involved_in_alternative_splicing_in_the_rat_beta_tropomyosin_gene:_the_3'_splice_site_of_the_skeletal_muscle_exon_7_is_the_major_site_of_blockage_in_nonmuscle_cells_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/21.20.4762 DB - PRIME DP - Unbound Medicine ER -