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[Correlation of IgA class antigliadin and antiendomysial antibodies (IgA-AGA-IgA-EMA) with intestinal histology in celiac disease].
Acta Gastroenterol Latinoam. 1993; 23(1):19-25.AG

Abstract

The aim of this work was to establish the diagnostic and follow up value of IgA-class antiendomysium (IgA-EmA) and IgA-class antigliadin (IgA-AGA) antibodies in celiac disease. Correlation with the intestinal histology at the different stages of the disease was evaluated, as well as its therapeutic monitoring ability. Fifty six children, twenty seven girls and twenty nine boys, aged six months to twelve years old, were studied. Thirty nine celiac children were al different diagnostic stages of the disease. Seventeen children with malabsorption symptoms and with normal intestinal histology were used as controls. Sixty blood samples were obtained simultaneously with the small intestinal biopsy. IgA-AGA (ELISA method) and IgA-EmA (immunofluorescent test performed over lower third Rhesus monkey esophagus) were determined in every blood sample. In 34 serum samples from patients with total or subtotal villous atrophy, two were negative for IgA-AGA and only one was negative for IgA-EmA. In 26 samples from patients with normal intestinal histology, two were positive for IgA-AGA and four were positive for IgA-EmA. The results for IgA-EmA had sensitivity 97%, specificity 84.6%, positive predictive value 89.2% and negative predictive value 95%. In the case of IgA-AGA were: sensitivity 94.1%, specificity 92.3%, positive predictive value 94.1%, negative predictive value 92.3%. IgA-AGA and IgA-EmA showed a high correlation with intestinal histology and are in combination powerful tools for the diagnosis and follow up of celiac patients. Besides, they provide a useful aid in the indication of a jejunal biopsy and in close monitoring of the dietary treatment compliance.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Hospital de Pediatría Juan P. Garrahan, Servicio de Gastroenterología, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

8237260

Citation

De Rosa, S, et al. "[Correlation of IgA Class Antigliadin and Antiendomysial Antibodies (IgA-AGA-IgA-EMA) With Intestinal Histology in Celiac Disease]." Acta Gastroenterologica Latinoamericana, vol. 23, no. 1, 1993, pp. 19-25.
De Rosa S, Litwin N, Dávila MT, et al. [Correlation of IgA class antigliadin and antiendomysial antibodies (IgA-AGA-IgA-EMA) with intestinal histology in celiac disease]. Acta Gastroenterol Latinoam. 1993;23(1):19-25.
De Rosa, S., Litwin, N., Dávila, M. T., Ruiz, J. A., Guastavino, E., Pini, A., & Queralt, A. M. (1993). [Correlation of IgA class antigliadin and antiendomysial antibodies (IgA-AGA-IgA-EMA) with intestinal histology in celiac disease]. Acta Gastroenterologica Latinoamericana, 23(1), 19-25.
De Rosa S, et al. [Correlation of IgA Class Antigliadin and Antiendomysial Antibodies (IgA-AGA-IgA-EMA) With Intestinal Histology in Celiac Disease]. Acta Gastroenterol Latinoam. 1993;23(1):19-25. PubMed PMID: 8237260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Correlation of IgA class antigliadin and antiendomysial antibodies (IgA-AGA-IgA-EMA) with intestinal histology in celiac disease]. AU - De Rosa,S, AU - Litwin,N, AU - Dávila,M T, AU - Ruiz,J A, AU - Guastavino,E, AU - Pini,A, AU - Queralt,A M, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 19 EP - 25 JF - Acta gastroenterologica Latinoamericana JO - Acta Gastroenterol Latinoam VL - 23 IS - 1 N2 - The aim of this work was to establish the diagnostic and follow up value of IgA-class antiendomysium (IgA-EmA) and IgA-class antigliadin (IgA-AGA) antibodies in celiac disease. Correlation with the intestinal histology at the different stages of the disease was evaluated, as well as its therapeutic monitoring ability. Fifty six children, twenty seven girls and twenty nine boys, aged six months to twelve years old, were studied. Thirty nine celiac children were al different diagnostic stages of the disease. Seventeen children with malabsorption symptoms and with normal intestinal histology were used as controls. Sixty blood samples were obtained simultaneously with the small intestinal biopsy. IgA-AGA (ELISA method) and IgA-EmA (immunofluorescent test performed over lower third Rhesus monkey esophagus) were determined in every blood sample. In 34 serum samples from patients with total or subtotal villous atrophy, two were negative for IgA-AGA and only one was negative for IgA-EmA. In 26 samples from patients with normal intestinal histology, two were positive for IgA-AGA and four were positive for IgA-EmA. The results for IgA-EmA had sensitivity 97%, specificity 84.6%, positive predictive value 89.2% and negative predictive value 95%. In the case of IgA-AGA were: sensitivity 94.1%, specificity 92.3%, positive predictive value 94.1%, negative predictive value 92.3%. IgA-AGA and IgA-EmA showed a high correlation with intestinal histology and are in combination powerful tools for the diagnosis and follow up of celiac patients. Besides, they provide a useful aid in the indication of a jejunal biopsy and in close monitoring of the dietary treatment compliance.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0300-9033 UR - https://www.unboundmedicine.com/medline/citation/8237260/[Correlation_of_IgA_class_antigliadin_and_antiendomysial_antibodies__IgA_AGA_IgA_EMA__with_intestinal_histology_in_celiac_disease]_ L2 - http://www.diseaseinfosearch.org/result/1186 DB - PRIME DP - Unbound Medicine ER -