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Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction.
J Infect Dis. 1993 Dec; 168(6):1570-5.JI

Abstract

In the past several years, microsporidia have become recognized as another important group of opportunistic infections of immunocompromised patients, especially those with AIDS. Enteric infections with the noncultivatable microsporidian parasite Enterocytozoon bieneusi have been diagnosed from AIDS patients with chronic diarrhea, malabsorption, and wasting. The incidence of infection and mechanism of transmission of these organisms in humans is unknown. Several recent tests for human pathogens have been developed using rRNA genes as diagnostic probes. Using the polymerase chain reaction and conserved regions of the small subunit rRNA (SSU-rRNA) gene, the SSU-rRNA gene of E. bieneusi was successfully cloned and subsequently sequenced. Amplification of E. bieneusi rRNA could be demonstrated from intestinal biopsies from HIV-1-infected patients infected with E. bieneusi but not from intestinal biopsies from noninfected patients. This cloned SSU-rRNA gene was used to develop improved probes for detection of E. bieneusi in tissue of infected patients.

Authors+Show Affiliations

Department of Pathology (Division of Parasitology and Tropical Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8245549

Citation

Zhu, X, et al. "Small Subunit rRNA Sequence of Enterocytozoon Bieneusi and Its Potential Diagnostic Role With Use of the Polymerase Chain Reaction." The Journal of Infectious Diseases, vol. 168, no. 6, 1993, pp. 1570-5.
Zhu X, Wittner M, Tanowitz HB, et al. Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction. J Infect Dis. 1993;168(6):1570-5.
Zhu, X., Wittner, M., Tanowitz, H. B., Kotler, D., Cali, A., & Weiss, L. M. (1993). Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction. The Journal of Infectious Diseases, 168(6), 1570-5.
Zhu X, et al. Small Subunit rRNA Sequence of Enterocytozoon Bieneusi and Its Potential Diagnostic Role With Use of the Polymerase Chain Reaction. J Infect Dis. 1993;168(6):1570-5. PubMed PMID: 8245549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Small subunit rRNA sequence of Enterocytozoon bieneusi and its potential diagnostic role with use of the polymerase chain reaction. AU - Zhu,X, AU - Wittner,M, AU - Tanowitz,H B, AU - Kotler,D, AU - Cali,A, AU - Weiss,L M, PY - 1993/12/1/pubmed PY - 1993/12/1/medline PY - 1993/12/1/entrez SP - 1570 EP - 5 JF - The Journal of infectious diseases JO - J Infect Dis VL - 168 IS - 6 N2 - In the past several years, microsporidia have become recognized as another important group of opportunistic infections of immunocompromised patients, especially those with AIDS. Enteric infections with the noncultivatable microsporidian parasite Enterocytozoon bieneusi have been diagnosed from AIDS patients with chronic diarrhea, malabsorption, and wasting. The incidence of infection and mechanism of transmission of these organisms in humans is unknown. Several recent tests for human pathogens have been developed using rRNA genes as diagnostic probes. Using the polymerase chain reaction and conserved regions of the small subunit rRNA (SSU-rRNA) gene, the SSU-rRNA gene of E. bieneusi was successfully cloned and subsequently sequenced. Amplification of E. bieneusi rRNA could be demonstrated from intestinal biopsies from HIV-1-infected patients infected with E. bieneusi but not from intestinal biopsies from noninfected patients. This cloned SSU-rRNA gene was used to develop improved probes for detection of E. bieneusi in tissue of infected patients. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/8245549/Small_subunit_rRNA_sequence_of_Enterocytozoon_bieneusi_and_its_potential_diagnostic_role_with_use_of_the_polymerase_chain_reaction_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/168.6.1570 DB - PRIME DP - Unbound Medicine ER -