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Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region.
J Gen Virol. 1993 Nov; 74 (Pt 11):2391-9.JG

Abstract

Hepatitis C virus (HCV) showed substantial nucleotide sequence diversity distributed throughout the viral genome, with many variants showing only 68 to 79% overall sequence similarity to one another. Phylogenetic analysis of nucleotide sequences derived from part of the gene encoding a non-structural protein (NS-5) has provided evidence for six major genotypes of HCV amongst a worldwide collection of 76 samples from HCV-infected blood donors and patients with chronic hepatitis. Many of these HCV types comprised a number of more closely related subtypes, leading to a current total of 11 genetically distinct viral populations. Phylogenetic analysis of other regions of the viral genome produced relationships between published sequences equivalent to those found in NS-5, apart from the more highly conserved 5' non-coding region in which only the six major HCV types, but not subtypes, could be differentiated. A new nomenclature for HCV variants is proposed in this communication that reflects the two-tiered nature of sequence differences between different viral isolates. The scheme classifies all known HCV variants to date, and describes criteria that would enable new variants to be assigned within the classification as they are discovered.

Authors+Show Affiliations

Department of Medical Microbiology, Medical School, University of Edinburgh, U.K.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8245854

Citation

Simmonds, P, et al. "Classification of Hepatitis C Virus Into Six Major Genotypes and a Series of Subtypes By Phylogenetic Analysis of the NS-5 Region." The Journal of General Virology, vol. 74 (Pt 11), 1993, pp. 2391-9.
Simmonds P, Holmes EC, Cha TA, et al. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. J Gen Virol. 1993;74 (Pt 11):2391-9.
Simmonds, P., Holmes, E. C., Cha, T. A., Chan, S. W., McOmish, F., Irvine, B., Beall, E., Yap, P. L., Kolberg, J., & Urdea, M. S. (1993). Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. The Journal of General Virology, 74 (Pt 11), 2391-9.
Simmonds P, et al. Classification of Hepatitis C Virus Into Six Major Genotypes and a Series of Subtypes By Phylogenetic Analysis of the NS-5 Region. J Gen Virol. 1993;74 (Pt 11):2391-9. PubMed PMID: 8245854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. AU - Simmonds,P, AU - Holmes,E C, AU - Cha,T A, AU - Chan,S W, AU - McOmish,F, AU - Irvine,B, AU - Beall,E, AU - Yap,P L, AU - Kolberg,J, AU - Urdea,M S, PY - 1993/11/1/pubmed PY - 1993/11/1/medline PY - 1993/11/1/entrez SP - 2391 EP - 9 JF - The Journal of general virology JO - J Gen Virol VL - 74 (Pt 11) N2 - Hepatitis C virus (HCV) showed substantial nucleotide sequence diversity distributed throughout the viral genome, with many variants showing only 68 to 79% overall sequence similarity to one another. Phylogenetic analysis of nucleotide sequences derived from part of the gene encoding a non-structural protein (NS-5) has provided evidence for six major genotypes of HCV amongst a worldwide collection of 76 samples from HCV-infected blood donors and patients with chronic hepatitis. Many of these HCV types comprised a number of more closely related subtypes, leading to a current total of 11 genetically distinct viral populations. Phylogenetic analysis of other regions of the viral genome produced relationships between published sequences equivalent to those found in NS-5, apart from the more highly conserved 5' non-coding region in which only the six major HCV types, but not subtypes, could be differentiated. A new nomenclature for HCV variants is proposed in this communication that reflects the two-tiered nature of sequence differences between different viral isolates. The scheme classifies all known HCV variants to date, and describes criteria that would enable new variants to be assigned within the classification as they are discovered. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/8245854/Classification_of_hepatitis_C_virus_into_six_major_genotypes_and_a_series_of_subtypes_by_phylogenetic_analysis_of_the_NS_5_region_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/0022-1317-74-11-2391 DB - PRIME DP - Unbound Medicine ER -