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In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique.
Anal Biochem. 1993 Oct; 214(1):31-7.AB

Abstract

Transition from glucose limitation to glucose excess in Saccharomyces cerevisiae leads to a fast change from complete to partial oxidative metabolism. To determine rapid changes of in vivo concentrations of yeast metabolites, occurring in the range of seconds after a glucose injection, a fast sampling technique was developed. A harvesting device with negligible dead space and highly efficient inactivation and extraction steps was applied to analyze the behavior of the physiological concentrations of adenine nucleotides as the dynamic response to fast changing glucose concentrations. Adenine nucleotides were selected as appropriate references due to their high turnover rates (in the range of seconds). During the first 5 s after a glucose pulse to a continuous chemostat culture, a significant decrease (30%) in ATP concentration and a coincident rise of ADP and AMP values were found. A further advantage of this technique is the high sampling frequency (5 s) and the long-term sterility ensuring recurrent harvesting.

Authors+Show Affiliations

Institut für Bioverfahrenstechnik, Universität Stuttgart, Federal Republic of Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8250239

Citation

Theobald, U, et al. "In Vivo Analysis of Glucose-induced Fast Changes in Yeast Adenine Nucleotide Pool Applying a Rapid Sampling Technique." Analytical Biochemistry, vol. 214, no. 1, 1993, pp. 31-7.
Theobald U, Mailinger W, Reuss M, et al. In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique. Anal Biochem. 1993;214(1):31-7.
Theobald, U., Mailinger, W., Reuss, M., & Rizzi, M. (1993). In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique. Analytical Biochemistry, 214(1), 31-7.
Theobald U, et al. In Vivo Analysis of Glucose-induced Fast Changes in Yeast Adenine Nucleotide Pool Applying a Rapid Sampling Technique. Anal Biochem. 1993;214(1):31-7. PubMed PMID: 8250239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique. AU - Theobald,U, AU - Mailinger,W, AU - Reuss,M, AU - Rizzi,M, PY - 1993/10/1/pubmed PY - 1993/10/1/medline PY - 1993/10/1/entrez SP - 31 EP - 7 JF - Analytical biochemistry JO - Anal Biochem VL - 214 IS - 1 N2 - Transition from glucose limitation to glucose excess in Saccharomyces cerevisiae leads to a fast change from complete to partial oxidative metabolism. To determine rapid changes of in vivo concentrations of yeast metabolites, occurring in the range of seconds after a glucose injection, a fast sampling technique was developed. A harvesting device with negligible dead space and highly efficient inactivation and extraction steps was applied to analyze the behavior of the physiological concentrations of adenine nucleotides as the dynamic response to fast changing glucose concentrations. Adenine nucleotides were selected as appropriate references due to their high turnover rates (in the range of seconds). During the first 5 s after a glucose pulse to a continuous chemostat culture, a significant decrease (30%) in ATP concentration and a coincident rise of ADP and AMP values were found. A further advantage of this technique is the high sampling frequency (5 s) and the long-term sterility ensuring recurrent harvesting. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/8250239/In_vivo_analysis_of_glucose_induced_fast_changes_in_yeast_adenine_nucleotide_pool_applying_a_rapid_sampling_technique_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(83)71452-1 DB - PRIME DP - Unbound Medicine ER -