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In vitro expression of osteoblastic markers in cells isolated from normal fetal and postnatal human bone and from bone of patients with osteogenesis imperfecta.
J Cell Physiol. 1993 Dec; 157(3):439-44.JC

Abstract

We studied the expression of osteoblastic markers in cultured cells isolated from the bone of 15 patients with different clinical forms of osteogenesis imperfecta (OI) and of seven fetal and postnatal controls. Cultured bone cells of ten OI patients produced abnormal collagen type I. Similar to controls, OI bone cells produced predominantly collagen type I with traces of collagen types III and V. The 1,25(OH)2 vitamin D3-stimulated synthesis of osteocalcin, a specific osteoblastic marker protein, was similar in OI bone cells and age-matched controls. Bone cells from fetal controls and from patients with the perinatal lethal OI type II produced less osteocalcin than bone cells from postnatal controls and surviving OI patients. OI bone cells responded to parathyroid hormone (PTH) by increased production of cAMP similar to controls. Bone cells from fetal controls and from OI type II donors showed a decreased response to PTH. Activity of the bone-liver-kidney isoenzyme alkaline phosphatase (AP) was detected in all control and OI bone cells. The expression of all osteoblastic markers was similar in bone cells producing abnormal collagen type I. These observations show that OI bone cells in vitro express a pattern of osteoblastic markers similar to age-matched control bone cells indicating that osteoblastic differentiation is not altered by the underlying defects of collagen type I metabolism in OI bone cells.

Authors+Show Affiliations

Department of Pediatrics, University of Ulm, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8253854

Citation

Mörike, M, et al. "In Vitro Expression of Osteoblastic Markers in Cells Isolated From Normal Fetal and Postnatal Human Bone and From Bone of Patients With Osteogenesis Imperfecta." Journal of Cellular Physiology, vol. 157, no. 3, 1993, pp. 439-44.
Mörike M, Schulz M, Brenner RE, et al. In vitro expression of osteoblastic markers in cells isolated from normal fetal and postnatal human bone and from bone of patients with osteogenesis imperfecta. J Cell Physiol. 1993;157(3):439-44.
Mörike, M., Schulz, M., Brenner, R. E., Bushart, G. B., Teller, W. M., & Vetter, U. (1993). In vitro expression of osteoblastic markers in cells isolated from normal fetal and postnatal human bone and from bone of patients with osteogenesis imperfecta. Journal of Cellular Physiology, 157(3), 439-44.
Mörike M, et al. In Vitro Expression of Osteoblastic Markers in Cells Isolated From Normal Fetal and Postnatal Human Bone and From Bone of Patients With Osteogenesis Imperfecta. J Cell Physiol. 1993;157(3):439-44. PubMed PMID: 8253854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro expression of osteoblastic markers in cells isolated from normal fetal and postnatal human bone and from bone of patients with osteogenesis imperfecta. AU - Mörike,M, AU - Schulz,M, AU - Brenner,R E, AU - Bushart,G B, AU - Teller,W M, AU - Vetter,U, PY - 1993/12/1/pubmed PY - 1993/12/1/medline PY - 1993/12/1/entrez SP - 439 EP - 44 JF - Journal of cellular physiology JO - J Cell Physiol VL - 157 IS - 3 N2 - We studied the expression of osteoblastic markers in cultured cells isolated from the bone of 15 patients with different clinical forms of osteogenesis imperfecta (OI) and of seven fetal and postnatal controls. Cultured bone cells of ten OI patients produced abnormal collagen type I. Similar to controls, OI bone cells produced predominantly collagen type I with traces of collagen types III and V. The 1,25(OH)2 vitamin D3-stimulated synthesis of osteocalcin, a specific osteoblastic marker protein, was similar in OI bone cells and age-matched controls. Bone cells from fetal controls and from patients with the perinatal lethal OI type II produced less osteocalcin than bone cells from postnatal controls and surviving OI patients. OI bone cells responded to parathyroid hormone (PTH) by increased production of cAMP similar to controls. Bone cells from fetal controls and from OI type II donors showed a decreased response to PTH. Activity of the bone-liver-kidney isoenzyme alkaline phosphatase (AP) was detected in all control and OI bone cells. The expression of all osteoblastic markers was similar in bone cells producing abnormal collagen type I. These observations show that OI bone cells in vitro express a pattern of osteoblastic markers similar to age-matched control bone cells indicating that osteoblastic differentiation is not altered by the underlying defects of collagen type I metabolism in OI bone cells. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/8253854/In_vitro_expression_of_osteoblastic_markers_in_cells_isolated_from_normal_fetal_and_postnatal_human_bone_and_from_bone_of_patients_with_osteogenesis_imperfecta_ DB - PRIME DP - Unbound Medicine ER -