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Preliminary X-ray crystallographic study of the proteasome from Thermoplasma acidophilum.
J Mol Biol. 1993 Dec 05; 234(3):881-4.JM

Abstract

Single cystals of proteasomes from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapor diffusion method. The crystals diffract to better than 3.0 A and belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 308.9 A, b = 208.8 A and c = 116.9 A. There is one molecular complex in the asymmetric unit. Two potentially useful heavy-atom derivatives have been obtained. The self-rotation function of the native Patterson map shows local sevenfold symmetry, consistent with the low-resolution structure obtained by electron microscopic techniques. The unit cell dimensions and crystal symmetry together with the shape and size of the proteasome suggest a packing arrangement of proteasome molecules in the unit cell, with their cylinder axis nearly parallel to the crystallographic a-axis.

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Authors+Show Affiliations

Jap B
Max-Planck-Institut für Biochemie, Martinsried bei München, Germany.
Pühler G
No affiliation info available
Lücke H
No affiliation info available
Typke D
No affiliation info available
Löwe J
No affiliation info available
Stock D
No affiliation info available
Huber R
No affiliation info available
Baumeister W
No affiliation info available

MeSH

CrystallizationCrystallography, X-RayCysteine EndopeptidasesMultienzyme ComplexesProteasome Endopeptidase ComplexProtein ConformationThermoplasma

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8254679

Citation

Jap, B, et al. "Preliminary X-ray Crystallographic Study of the Proteasome From Thermoplasma Acidophilum." Journal of Molecular Biology, vol. 234, no. 3, 1993, pp. 881-4.
Jap B, Pühler G, Lücke H, et al. Preliminary X-ray crystallographic study of the proteasome from Thermoplasma acidophilum. J Mol Biol. 1993;234(3):881-4.
Jap, B., Pühler, G., Lücke, H., Typke, D., Löwe, J., Stock, D., Huber, R., & Baumeister, W. (1993). Preliminary X-ray crystallographic study of the proteasome from Thermoplasma acidophilum. Journal of Molecular Biology, 234(3), 881-4.
Jap B, et al. Preliminary X-ray Crystallographic Study of the Proteasome From Thermoplasma Acidophilum. J Mol Biol. 1993 Dec 5;234(3):881-4. PubMed PMID: 8254679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preliminary X-ray crystallographic study of the proteasome from Thermoplasma acidophilum. AU - Jap,B, AU - Pühler,G, AU - Lücke,H, AU - Typke,D, AU - Löwe,J, AU - Stock,D, AU - Huber,R, AU - Baumeister,W, PY - 1993/12/5/pubmed PY - 2000/6/1/medline PY - 1993/12/5/entrez SP - 881 EP - 4 JF - Journal of molecular biology JO - J Mol Biol VL - 234 IS - 3 N2 - Single cystals of proteasomes from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapor diffusion method. The crystals diffract to better than 3.0 A and belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 308.9 A, b = 208.8 A and c = 116.9 A. There is one molecular complex in the asymmetric unit. Two potentially useful heavy-atom derivatives have been obtained. The self-rotation function of the native Patterson map shows local sevenfold symmetry, consistent with the low-resolution structure obtained by electron microscopic techniques. The unit cell dimensions and crystal symmetry together with the shape and size of the proteasome suggest a packing arrangement of proteasome molecules in the unit cell, with their cylinder axis nearly parallel to the crystallographic a-axis. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/8254679/Preliminary_X_ray_crystallographic_study_of_the_proteasome_from_Thermoplasma_acidophilum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(83)71633-5 DB - PRIME DP - Unbound Medicine ER -