Tags

Type your tag names separated by a space and hit enter

Effects of endotoxin in vivo on endothelial and smooth-muscle function in rabbit and rat aorta.
Am Rev Respir Dis. 1993 Dec; 148(6 Pt 1):1638-45.AR

Abstract

In order to determine whether endotoxemia induced generalized defects in vascular contraction and endothelium-dependent relaxation, we studied the effect of in vivo endotoxin administration in Sprague-Dawley rats and New Zealand White rabbits on endothelial and arterial smooth-muscle responses of isolated thoracic aorta in vitro. Endotoxin treatment significantly decreased contractile responses to phenylephrine (PE), angiotensin II (AII), serotonin (5-HT), and potassium chloride. This effect was not altered by indomethacin or endothelial denudation. Treatment of vessels with NG-nitro-L-arginine (NNLA), an inhibitor of arginine-dependent nitric oxide biosynthesis, or with methylene blue, an inhibitor of soluble guanylate cyclase, resulted in significant improvement of the contractile defect in endotoxin-treated vessels. The restorative effect of NNLA on contractile responses in endotoxin-treated aortic rings was similar in the presence or absence of an intact endothelium. Endothelium-dependent relaxation in response to acetylcholine, substance P, or the calcium ionophore A23187 was markedly impaired in vessels from endotoxin-treated rabbits, while endothelium-independent relaxation in response to nitroprusside was similar in both groups. These results suggest that endotoxemia both induces basal, nonendothelial nitric oxide synthesis and impairs the agonist-stimulated release of endothelium-derived relaxing factor (EDRF). These findings may have mechanistic importance in the hemodynamic derangements of endotoxemia.

Authors+Show Affiliations

Department of Medicine, University of Chicago, Illinois 60637.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8256913

Citation

Umans, J G., et al. "Effects of Endotoxin in Vivo On Endothelial and Smooth-muscle Function in Rabbit and Rat Aorta." The American Review of Respiratory Disease, vol. 148, no. 6 Pt 1, 1993, pp. 1638-45.
Umans JG, Wylam ME, Samsel RW, et al. Effects of endotoxin in vivo on endothelial and smooth-muscle function in rabbit and rat aorta. Am Rev Respir Dis. 1993;148(6 Pt 1):1638-45.
Umans, J. G., Wylam, M. E., Samsel, R. W., Edwards, J., & Schumacker, P. T. (1993). Effects of endotoxin in vivo on endothelial and smooth-muscle function in rabbit and rat aorta. The American Review of Respiratory Disease, 148(6 Pt 1), 1638-45.
Umans JG, et al. Effects of Endotoxin in Vivo On Endothelial and Smooth-muscle Function in Rabbit and Rat Aorta. Am Rev Respir Dis. 1993;148(6 Pt 1):1638-45. PubMed PMID: 8256913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of endotoxin in vivo on endothelial and smooth-muscle function in rabbit and rat aorta. AU - Umans,J G, AU - Wylam,M E, AU - Samsel,R W, AU - Edwards,J, AU - Schumacker,P T, PY - 1993/12/1/pubmed PY - 1993/12/1/medline PY - 1993/12/1/entrez SP - 1638 EP - 45 JF - The American review of respiratory disease JO - Am Rev Respir Dis VL - 148 IS - 6 Pt 1 N2 - In order to determine whether endotoxemia induced generalized defects in vascular contraction and endothelium-dependent relaxation, we studied the effect of in vivo endotoxin administration in Sprague-Dawley rats and New Zealand White rabbits on endothelial and arterial smooth-muscle responses of isolated thoracic aorta in vitro. Endotoxin treatment significantly decreased contractile responses to phenylephrine (PE), angiotensin II (AII), serotonin (5-HT), and potassium chloride. This effect was not altered by indomethacin or endothelial denudation. Treatment of vessels with NG-nitro-L-arginine (NNLA), an inhibitor of arginine-dependent nitric oxide biosynthesis, or with methylene blue, an inhibitor of soluble guanylate cyclase, resulted in significant improvement of the contractile defect in endotoxin-treated vessels. The restorative effect of NNLA on contractile responses in endotoxin-treated aortic rings was similar in the presence or absence of an intact endothelium. Endothelium-dependent relaxation in response to acetylcholine, substance P, or the calcium ionophore A23187 was markedly impaired in vessels from endotoxin-treated rabbits, while endothelium-independent relaxation in response to nitroprusside was similar in both groups. These results suggest that endotoxemia both induces basal, nonendothelial nitric oxide synthesis and impairs the agonist-stimulated release of endothelium-derived relaxing factor (EDRF). These findings may have mechanistic importance in the hemodynamic derangements of endotoxemia. SN - 0003-0805 UR - https://www.unboundmedicine.com/medline/citation/8256913/Effects_of_endotoxin_in_vivo_on_endothelial_and_smooth-muscle_function_in_rabbit_and_rat_aorta. DB - PRIME DP - Unbound Medicine ER -