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The effects of inhaled interferon gamma in normal human airways.
Am Rev Respir Dis. 1993 Dec; 148(6 Pt 1):1677-82.AR

Abstract

Recent studies suggest that cytokines such as recombinant interferon-gamma (rIFN-gamma) may play a role in the treatment of certain respiratory conditions associated with infection and inflammation. This study was designed to determine if rIFN-gamma could be delivered effectively in a group of normal human volunteers. The effectiveness of the inhaled delivery system was demonstrated by the recovery of free IFN-gamma in bronchoalveolar lavage (BAL) fluid and macrophage (M phi) expression of IP-10, an IFN-gamma-inducible molecule, after therapy but not at baseline. IL-1 beta, but not IL-8, gene transcripts also showed evidence for up-regulation after rIFN-gamma therapy. Compared with baseline, inhaled rIFN-gamma did not significantly alter clinical symptom scores, spirometry, morning peak expiratory flow rate (PEFR), or the response to methacholine. Of interest, the evening PEFR increased significantly (p = 0.02), from 568 +/- 36 L/min at baseline to 584 +/- 33 L/min after inhaled rIFN-gamma. Although there was no significant change in total white cell count in BAL fluid, the cellular composition did demonstrate a significant decrease in percentage of alveolar M phi (p = 0.02) and an increase in percentage of lymphocytes (p = 0.02) after rIFN-gamma. There were no histologic differences seen in bronchial biopsy specimens, and there was no evidence for up-regulation of ICAM-1 or HLA-DR expression after rIFN-gamma. We conclude that, in normal persons, rIFN-gamma can be effectively delivered by inhalation. Future trials using inhaled rIFN-gamma appear to be warranted for certain pulmonary diseases.

Authors+Show Affiliations

Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8256919

Citation

Martin, R J., et al. "The Effects of Inhaled Interferon Gamma in Normal Human Airways." The American Review of Respiratory Disease, vol. 148, no. 6 Pt 1, 1993, pp. 1677-82.
Martin RJ, Boguniewicz M, Henson JE, et al. The effects of inhaled interferon gamma in normal human airways. Am Rev Respir Dis. 1993;148(6 Pt 1):1677-82.
Martin, R. J., Boguniewicz, M., Henson, J. E., Celniker, A. C., Williams, M., Giorno, R. C., & Leung, D. Y. (1993). The effects of inhaled interferon gamma in normal human airways. The American Review of Respiratory Disease, 148(6 Pt 1), 1677-82.
Martin RJ, et al. The Effects of Inhaled Interferon Gamma in Normal Human Airways. Am Rev Respir Dis. 1993;148(6 Pt 1):1677-82. PubMed PMID: 8256919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of inhaled interferon gamma in normal human airways. AU - Martin,R J, AU - Boguniewicz,M, AU - Henson,J E, AU - Celniker,A C, AU - Williams,M, AU - Giorno,R C, AU - Leung,D Y, PY - 1993/12/1/pubmed PY - 1993/12/1/medline PY - 1993/12/1/entrez SP - 1677 EP - 82 JF - The American review of respiratory disease JO - Am Rev Respir Dis VL - 148 IS - 6 Pt 1 N2 - Recent studies suggest that cytokines such as recombinant interferon-gamma (rIFN-gamma) may play a role in the treatment of certain respiratory conditions associated with infection and inflammation. This study was designed to determine if rIFN-gamma could be delivered effectively in a group of normal human volunteers. The effectiveness of the inhaled delivery system was demonstrated by the recovery of free IFN-gamma in bronchoalveolar lavage (BAL) fluid and macrophage (M phi) expression of IP-10, an IFN-gamma-inducible molecule, after therapy but not at baseline. IL-1 beta, but not IL-8, gene transcripts also showed evidence for up-regulation after rIFN-gamma therapy. Compared with baseline, inhaled rIFN-gamma did not significantly alter clinical symptom scores, spirometry, morning peak expiratory flow rate (PEFR), or the response to methacholine. Of interest, the evening PEFR increased significantly (p = 0.02), from 568 +/- 36 L/min at baseline to 584 +/- 33 L/min after inhaled rIFN-gamma. Although there was no significant change in total white cell count in BAL fluid, the cellular composition did demonstrate a significant decrease in percentage of alveolar M phi (p = 0.02) and an increase in percentage of lymphocytes (p = 0.02) after rIFN-gamma. There were no histologic differences seen in bronchial biopsy specimens, and there was no evidence for up-regulation of ICAM-1 or HLA-DR expression after rIFN-gamma. We conclude that, in normal persons, rIFN-gamma can be effectively delivered by inhalation. Future trials using inhaled rIFN-gamma appear to be warranted for certain pulmonary diseases. SN - 0003-0805 UR - https://www.unboundmedicine.com/medline/citation/8256919/The_effects_of_inhaled_interferon_gamma_in_normal_human_airways. L2 - https://www.atsjournals.org/doi/10.1164/ajrccm/148.6_Pt_1.1677?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -