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Lipoprotein lipases, lipoprotein density gradient profile and LDL receptor activity in miniature pigs fed fish oil and corn oil.
Biochim Biophys Acta 1993; 1210(1):113-22BB

Abstract

The effects of fish oil and corn oil on plasma lipoprotein concentrations, the lipolytic enzymes, lipoprotein lipase and hepatic triacylglycerol lipase, the density distribution of the plasma lipoproteins and LDL receptor activity were studied. These experiments were designed, in part, to define the mechanism(s) responsible for the increased conversion of plasma VLDL apolipoprotein B to LDL and a decreased LDL apolipoprotein B fractional catabolic rate described in previous apolipoprotein B kinetic studies. Miniature pigs were fed diets for 3 to 6 weeks containing supplements of corn oil or fish oil as Maxepa. Triacylglycerol and cholesterol in plasma and VLDL were significantly reduced by the fish oil diet. LDL and HDL cholesterol were not significantly changed. The fish oil diet significantly reduced post-heparin plasma lipoprotein lipase and hepatic triacylglycerol lipase activities, which may be an adaptive response to the low concentration of substrates (triacylglycerol-rich lipoproteins) for these enzymes. No differences were observed in the density of VLDL, LDL or HDL as determined by density gradient ultracentrifugation with the fish oil diet. No major changes in percent lipid composition of VLDL, LDL and HDL were observed. No differences were found with respect to LDL uptake by J774 macrophages. Receptor mediated clearance of LDL in vivo, as assessed by measuring the difference in fractional catabolic rate of native vs. methylated LDL decreased significantly by 17% (P < 0.032). We conclude that the increased conversion of VLDL apolipoprotein B to LDL in miniature pigs fed fish oil is not related to an increase in lipolytic enzymes or density distribution of VLDL, but may be due in part to a decrease in LDL receptor activity.

Authors+Show Affiliations

Department of Medicine, University of Western Ontario, London, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8257713

Citation

Huff, M W., et al. "Lipoprotein Lipases, Lipoprotein Density Gradient Profile and LDL Receptor Activity in Miniature Pigs Fed Fish Oil and Corn Oil." Biochimica Et Biophysica Acta, vol. 1210, no. 1, 1993, pp. 113-22.
Huff MW, Telford DE, Edmonds BW, et al. Lipoprotein lipases, lipoprotein density gradient profile and LDL receptor activity in miniature pigs fed fish oil and corn oil. Biochim Biophys Acta. 1993;1210(1):113-22.
Huff, M. W., Telford, D. E., Edmonds, B. W., McDonald, C. G., & Evans, A. J. (1993). Lipoprotein lipases, lipoprotein density gradient profile and LDL receptor activity in miniature pigs fed fish oil and corn oil. Biochimica Et Biophysica Acta, 1210(1), pp. 113-22.
Huff MW, et al. Lipoprotein Lipases, Lipoprotein Density Gradient Profile and LDL Receptor Activity in Miniature Pigs Fed Fish Oil and Corn Oil. Biochim Biophys Acta. 1993 Dec 2;1210(1):113-22. PubMed PMID: 8257713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipoprotein lipases, lipoprotein density gradient profile and LDL receptor activity in miniature pigs fed fish oil and corn oil. AU - Huff,M W, AU - Telford,D E, AU - Edmonds,B W, AU - McDonald,C G, AU - Evans,A J, PY - 1993/12/2/pubmed PY - 1993/12/2/medline PY - 1993/12/2/entrez SP - 113 EP - 22 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 1210 IS - 1 N2 - The effects of fish oil and corn oil on plasma lipoprotein concentrations, the lipolytic enzymes, lipoprotein lipase and hepatic triacylglycerol lipase, the density distribution of the plasma lipoproteins and LDL receptor activity were studied. These experiments were designed, in part, to define the mechanism(s) responsible for the increased conversion of plasma VLDL apolipoprotein B to LDL and a decreased LDL apolipoprotein B fractional catabolic rate described in previous apolipoprotein B kinetic studies. Miniature pigs were fed diets for 3 to 6 weeks containing supplements of corn oil or fish oil as Maxepa. Triacylglycerol and cholesterol in plasma and VLDL were significantly reduced by the fish oil diet. LDL and HDL cholesterol were not significantly changed. The fish oil diet significantly reduced post-heparin plasma lipoprotein lipase and hepatic triacylglycerol lipase activities, which may be an adaptive response to the low concentration of substrates (triacylglycerol-rich lipoproteins) for these enzymes. No differences were observed in the density of VLDL, LDL or HDL as determined by density gradient ultracentrifugation with the fish oil diet. No major changes in percent lipid composition of VLDL, LDL and HDL were observed. No differences were found with respect to LDL uptake by J774 macrophages. Receptor mediated clearance of LDL in vivo, as assessed by measuring the difference in fractional catabolic rate of native vs. methylated LDL decreased significantly by 17% (P < 0.032). We conclude that the increased conversion of VLDL apolipoprotein B to LDL in miniature pigs fed fish oil is not related to an increase in lipolytic enzymes or density distribution of VLDL, but may be due in part to a decrease in LDL receptor activity. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/8257713/Lipoprotein_lipases_lipoprotein_density_gradient_profile_and_LDL_receptor_activity_in_miniature_pigs_fed_fish_oil_and_corn_oil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0005-2760(93)90056-F DB - PRIME DP - Unbound Medicine ER -