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Protein profile in bronchoalveolar lavage fluid from patients with sarcoidosis and idiopathic pulmonary fibrosis as revealed by SDS-PAGE electrophoresis and western blot analysis.
Scand J Clin Lab Invest. 1993 Oct; 53(6):617-23.SJ

Abstract

So far bronchoalveolar lavage (BAL)-protein in interstitial lung disease (ILD) is evaluated by measuring concentrations of single proteins. Due to the high dilution of most proteins in BAL, analysis of protein profile has been disappointing. This study describes a new method to overcome this problem and to reveal a highly differentiated picture of BAL proteins. Eighteen patients with pulmonary sarcoidosis, 18 patients with idiopathic pulmonary fibrosis (IPF) and 22 patients with no clinical, roentgenologic or functional evidence of ILD underwent BAL. Total and differential cell count was performed. Normal values for the control group, a lymphocytic alveolitis in sarcoidosis and a granulocytic alveolitis in IPF-patients were found. Median total protein concentration in sarcoidosis showed an increase five times higher than that of the controls (150 mg 1(-1) and 27 mg 1(-1), respectively) with p < 0.001, IPF protein concentration (58 mg 1(-1)) exceeded twice the control values (0.01 > p > 0.001). Analysis of electrophoretic protein profile in controls with Western blot analysis and the biotin/streptavidin staining system revealed a highly differentiated range of bands. Staining with immunoglobulin antibody identified six bands. Four proteins with molecular weight < 21.000 dalton were present only in sarcoidosis patients. These proteins may be identical with fragmented serum proteins or different cell mediators detected in alveolar cell supernatants. Furthermore, in sarcoidosis the intensity and number of bands with molecular weight more than 67.000 dalton was increased. This gives strong evidence for an injury of the alveolar membrane integrity in the alveolitis during the course of sarcoidosis.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Medizinische Klinik, Christian-Albrechts-Universität Kiel, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8266009

Citation

Uebelhoer, M, et al. "Protein Profile in Bronchoalveolar Lavage Fluid From Patients With Sarcoidosis and Idiopathic Pulmonary Fibrosis as Revealed By SDS-PAGE Electrophoresis and Western Blot Analysis." Scandinavian Journal of Clinical and Laboratory Investigation, vol. 53, no. 6, 1993, pp. 617-23.
Uebelhoer M, Bewig B, Oldigs M, et al. Protein profile in bronchoalveolar lavage fluid from patients with sarcoidosis and idiopathic pulmonary fibrosis as revealed by SDS-PAGE electrophoresis and western blot analysis. Scand J Clin Lab Invest. 1993;53(6):617-23.
Uebelhoer, M., Bewig, B., Oldigs, M., Nowak, D., Magnussen, H., Petermann, W., & Barth, J. (1993). Protein profile in bronchoalveolar lavage fluid from patients with sarcoidosis and idiopathic pulmonary fibrosis as revealed by SDS-PAGE electrophoresis and western blot analysis. Scandinavian Journal of Clinical and Laboratory Investigation, 53(6), 617-23.
Uebelhoer M, et al. Protein Profile in Bronchoalveolar Lavage Fluid From Patients With Sarcoidosis and Idiopathic Pulmonary Fibrosis as Revealed By SDS-PAGE Electrophoresis and Western Blot Analysis. Scand J Clin Lab Invest. 1993;53(6):617-23. PubMed PMID: 8266009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein profile in bronchoalveolar lavage fluid from patients with sarcoidosis and idiopathic pulmonary fibrosis as revealed by SDS-PAGE electrophoresis and western blot analysis. AU - Uebelhoer,M, AU - Bewig,B, AU - Oldigs,M, AU - Nowak,D, AU - Magnussen,H, AU - Petermann,W, AU - Barth,J, PY - 1993/10/1/pubmed PY - 1993/10/1/medline PY - 1993/10/1/entrez SP - 617 EP - 23 JF - Scandinavian journal of clinical and laboratory investigation JO - Scand J Clin Lab Invest VL - 53 IS - 6 N2 - So far bronchoalveolar lavage (BAL)-protein in interstitial lung disease (ILD) is evaluated by measuring concentrations of single proteins. Due to the high dilution of most proteins in BAL, analysis of protein profile has been disappointing. This study describes a new method to overcome this problem and to reveal a highly differentiated picture of BAL proteins. Eighteen patients with pulmonary sarcoidosis, 18 patients with idiopathic pulmonary fibrosis (IPF) and 22 patients with no clinical, roentgenologic or functional evidence of ILD underwent BAL. Total and differential cell count was performed. Normal values for the control group, a lymphocytic alveolitis in sarcoidosis and a granulocytic alveolitis in IPF-patients were found. Median total protein concentration in sarcoidosis showed an increase five times higher than that of the controls (150 mg 1(-1) and 27 mg 1(-1), respectively) with p < 0.001, IPF protein concentration (58 mg 1(-1)) exceeded twice the control values (0.01 > p > 0.001). Analysis of electrophoretic protein profile in controls with Western blot analysis and the biotin/streptavidin staining system revealed a highly differentiated range of bands. Staining with immunoglobulin antibody identified six bands. Four proteins with molecular weight < 21.000 dalton were present only in sarcoidosis patients. These proteins may be identical with fragmented serum proteins or different cell mediators detected in alveolar cell supernatants. Furthermore, in sarcoidosis the intensity and number of bands with molecular weight more than 67.000 dalton was increased. This gives strong evidence for an injury of the alveolar membrane integrity in the alveolitis during the course of sarcoidosis.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0036-5513 UR - https://www.unboundmedicine.com/medline/citation/8266009/Protein_profile_in_bronchoalveolar_lavage_fluid_from_patients_with_sarcoidosis_and_idiopathic_pulmonary_fibrosis_as_revealed_by_SDS_PAGE_electrophoresis_and_western_blot_analysis_ L2 - http://www.diseaseinfosearch.org/result/6087 DB - PRIME DP - Unbound Medicine ER -