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Strain identification and 5S rRNA gene characterization of the hyperthermophilic archaebacterium Sulfolobus acidocaldarius.
J Bacteriol. 1994 Jan; 176(2):514-7.JB

Abstract

A commonly used laboratory Sulfolobus strain has been unambiguously identified as Sulfolobus acidocaldarius DSM639. The 5S rRNA gene from this strain was cloned and sequenced. It differs at 17 of 124 positions from the identical 5S rRNA sequences from Sulfolobus solfataricus and a strain apparently misidentified as S. acidocaldarius. Analysis of the transcripts from the 5S rRNA gene failed to identify any precursor extending a significant distance beyond the 5' or 3' boundary of the 5S rRNA-coding sequence. This result suggests that the primary transcript of the 5S rRNA gene corresponds in length (within 1 or 2 nucleotides) to the mature 5S rRNA sequence found in 50S ribosomal subunits.

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Authors+Show Affiliations

Durovic P
Department of Biochemistry and Molecular Biology and Canadian Institute for Advanced Research, University of British Columbia, Vancouver, Canada.
Kutay U
No affiliation info available
Schleper C
No affiliation info available
Dennis PP
No affiliation info available

MeSH

Base SequenceMolecular Sequence DataNucleic Acid ConformationRNA PrecursorsRNA, Ribosomal, 5SSequence Homology, Nucleic AcidSpecies SpecificitySulfolobusSulfolobus acidocaldarius

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8288546

Citation

Durovic, P, et al. "Strain Identification and 5S rRNA Gene Characterization of the Hyperthermophilic Archaebacterium Sulfolobus Acidocaldarius." Journal of Bacteriology, vol. 176, no. 2, 1994, pp. 514-7.
Durovic P, Kutay U, Schleper C, et al. Strain identification and 5S rRNA gene characterization of the hyperthermophilic archaebacterium Sulfolobus acidocaldarius. J Bacteriol. 1994;176(2):514-7.
Durovic, P., Kutay, U., Schleper, C., & Dennis, P. P. (1994). Strain identification and 5S rRNA gene characterization of the hyperthermophilic archaebacterium Sulfolobus acidocaldarius. Journal of Bacteriology, 176(2), 514-7.
Durovic P, et al. Strain Identification and 5S rRNA Gene Characterization of the Hyperthermophilic Archaebacterium Sulfolobus Acidocaldarius. J Bacteriol. 1994;176(2):514-7. PubMed PMID: 8288546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Strain identification and 5S rRNA gene characterization of the hyperthermophilic archaebacterium Sulfolobus acidocaldarius. AU - Durovic,P, AU - Kutay,U, AU - Schleper,C, AU - Dennis,P P, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 514 EP - 7 JF - Journal of bacteriology JO - J Bacteriol VL - 176 IS - 2 N2 - A commonly used laboratory Sulfolobus strain has been unambiguously identified as Sulfolobus acidocaldarius DSM639. The 5S rRNA gene from this strain was cloned and sequenced. It differs at 17 of 124 positions from the identical 5S rRNA sequences from Sulfolobus solfataricus and a strain apparently misidentified as S. acidocaldarius. Analysis of the transcripts from the 5S rRNA gene failed to identify any precursor extending a significant distance beyond the 5' or 3' boundary of the 5S rRNA-coding sequence. This result suggests that the primary transcript of the 5S rRNA gene corresponds in length (within 1 or 2 nucleotides) to the mature 5S rRNA sequence found in 50S ribosomal subunits. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/8288546/Strain_identification_and_5S_rRNA_gene_characterization_of_the_hyperthermophilic_archaebacterium_Sulfolobus_acidocaldarius_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=8288546 DB - PRIME DP - Unbound Medicine ER -