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Dietary deficiency of N-3 fatty acids alters rhodopsin content and function in the rat retina.
Invest Ophthalmol Vis Sci. 1994 Jan; 35(1):91-100.IO

Abstract

PURPOSE

To investigate the possibility that previously demonstrated reductions in photoreceptor sensitivity to light in n-3 fatty-acid-deficient rats can be explained by alterations in rhodopsin content and/or function.

METHODS

Sprague-Dawley rats were reared throughout gestation, lactation, and up to 24 weeks of age on a diet containing safflower oil (n-3 fatty-acid-deficient) or soybean oil as the sole source of lipids. Dark-adapted content and in vivo regeneration of rhodopsin after bleaching were measured by detergent extraction. The regeneration rate constants and number of photons absorbed by rhodopsin under steady-state bleach conditions were calculated from these values. The rate of metarhodopsin II (MII) formation in vitro was determined by flash bleaching extracted pigment and native rod outer segment membranes. Rod outer segment length and photoreceptor cell density were determined in histologic sections through the inferior central retina.

RESULTS

Dark-adapted rhodopsin content of retinas from rats reared on safflower oil was 12% to 15% higher than that of rats raised on soybean oil at every age measured. The rate of rhodopsin regeneration was significantly slower in rats reared on safflower oil while the level of steady-state bleach was the same. This meant that the rats reared on safflower oil absorbed about one half as many photons during light exposure. The rate of metarhodopsin II formation in vitro was unaffected by n-3 fatty acid deficiency. No difference in either rod outer segment length or cell number was detected.

CONCLUSION

A reduced capacity for photon absorption by rhodopsin could play a role in lowering retinal sensitivity to light in n-3 fatty-acid-deficient rats.

Authors+Show Affiliations

Department of Ophthalomology, University of Zürich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8300367

Citation

Bush, R A., et al. "Dietary Deficiency of N-3 Fatty Acids Alters Rhodopsin Content and Function in the Rat Retina." Investigative Ophthalmology & Visual Science, vol. 35, no. 1, 1994, pp. 91-100.
Bush RA, Malnoë A, Remé CE, et al. Dietary deficiency of N-3 fatty acids alters rhodopsin content and function in the rat retina. Invest Ophthalmol Vis Sci. 1994;35(1):91-100.
Bush, R. A., Malnoë, A., Remé, C. E., & Williams, T. P. (1994). Dietary deficiency of N-3 fatty acids alters rhodopsin content and function in the rat retina. Investigative Ophthalmology & Visual Science, 35(1), 91-100.
Bush RA, et al. Dietary Deficiency of N-3 Fatty Acids Alters Rhodopsin Content and Function in the Rat Retina. Invest Ophthalmol Vis Sci. 1994;35(1):91-100. PubMed PMID: 8300367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary deficiency of N-3 fatty acids alters rhodopsin content and function in the rat retina. AU - Bush,R A, AU - Malnoë,A, AU - Remé,C E, AU - Williams,T P, PY - 1994/1/1/pubmed PY - 1994/1/1/medline PY - 1994/1/1/entrez SP - 91 EP - 100 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 35 IS - 1 N2 - PURPOSE: To investigate the possibility that previously demonstrated reductions in photoreceptor sensitivity to light in n-3 fatty-acid-deficient rats can be explained by alterations in rhodopsin content and/or function. METHODS: Sprague-Dawley rats were reared throughout gestation, lactation, and up to 24 weeks of age on a diet containing safflower oil (n-3 fatty-acid-deficient) or soybean oil as the sole source of lipids. Dark-adapted content and in vivo regeneration of rhodopsin after bleaching were measured by detergent extraction. The regeneration rate constants and number of photons absorbed by rhodopsin under steady-state bleach conditions were calculated from these values. The rate of metarhodopsin II (MII) formation in vitro was determined by flash bleaching extracted pigment and native rod outer segment membranes. Rod outer segment length and photoreceptor cell density were determined in histologic sections through the inferior central retina. RESULTS: Dark-adapted rhodopsin content of retinas from rats reared on safflower oil was 12% to 15% higher than that of rats raised on soybean oil at every age measured. The rate of rhodopsin regeneration was significantly slower in rats reared on safflower oil while the level of steady-state bleach was the same. This meant that the rats reared on safflower oil absorbed about one half as many photons during light exposure. The rate of metarhodopsin II formation in vitro was unaffected by n-3 fatty acid deficiency. No difference in either rod outer segment length or cell number was detected. CONCLUSION: A reduced capacity for photon absorption by rhodopsin could play a role in lowering retinal sensitivity to light in n-3 fatty-acid-deficient rats. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/8300367/Dietary_deficiency_of_N_3_fatty_acids_alters_rhodopsin_content_and_function_in_the_rat_retina_ L2 - https://iovs.arvojournals.org/article.aspx?volume=35&issue=1&page=91 DB - PRIME DP - Unbound Medicine ER -