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The turnover of 57Co-labeled cyanocobalamin bound to cobalamin binding proteins in patients with chronic myelogenous leukemia.
J Lab Clin Med. 1994 Feb; 123(2):264-72.JL

Abstract

Individual plasma specimens from six patients with chronic myelogenous leukemia (CML) in chronic phase were incubated with [57Co]cyanocobalamin and injected into patients to study the turnover of cobalamin bound to haptocorrin and transcobalamin. The [57Co] radioactivity bound to haptocorrin and transcobalamin was determined after separating the proteins by adsorption to insolubilized antibodies to haptocorrin. The distribution of the radioactivity on the different forms of cobalamin and on haptocorrin isoproteins were determined. The fractional catabolic rate of hapatocorrin (0.102 to 0.158 d-1) and transcobalamin (3 to 29 d-1) was of the same magnitude as previously reported for a reference population. During the first 24 hours of the turnover, no change of the cyanocobalamin form was registered, indicating that conversion of cyanocobalamin to the coenzyme forms of cobalamin does not take place in the circulation. The haptocorrin isoprotein pattern changes in alkaline direction during the first 5 hours indicated that the glycoprotein was desialinated during circulation. From the calculated turnover parameter, the possible competition between transcobalamin and haptocorrin for the transport of cobalamins in CML is estimated to be of minor clinical significance. In conclusion, the increased plasma concentration of haptocorrin in CML is due to an increased liberation of haptocorrin and is not due to a decreased turnover of the protein.

Authors+Show Affiliations

Department of Internal Medicine and Hematology, Herlev Hospital, Denmark.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8301203

Citation

Gimsing, P, and E Nexø. "The Turnover of 57Co-labeled Cyanocobalamin Bound to Cobalamin Binding Proteins in Patients With Chronic Myelogenous Leukemia." The Journal of Laboratory and Clinical Medicine, vol. 123, no. 2, 1994, pp. 264-72.
Gimsing P, Nexø E. The turnover of 57Co-labeled cyanocobalamin bound to cobalamin binding proteins in patients with chronic myelogenous leukemia. J Lab Clin Med. 1994;123(2):264-72.
Gimsing, P., & Nexø, E. (1994). The turnover of 57Co-labeled cyanocobalamin bound to cobalamin binding proteins in patients with chronic myelogenous leukemia. The Journal of Laboratory and Clinical Medicine, 123(2), 264-72.
Gimsing P, Nexø E. The Turnover of 57Co-labeled Cyanocobalamin Bound to Cobalamin Binding Proteins in Patients With Chronic Myelogenous Leukemia. J Lab Clin Med. 1994;123(2):264-72. PubMed PMID: 8301203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The turnover of 57Co-labeled cyanocobalamin bound to cobalamin binding proteins in patients with chronic myelogenous leukemia. AU - Gimsing,P, AU - Nexø,E, PY - 1994/2/1/pubmed PY - 1994/2/1/medline PY - 1994/2/1/entrez SP - 264 EP - 72 JF - The Journal of laboratory and clinical medicine JO - J Lab Clin Med VL - 123 IS - 2 N2 - Individual plasma specimens from six patients with chronic myelogenous leukemia (CML) in chronic phase were incubated with [57Co]cyanocobalamin and injected into patients to study the turnover of cobalamin bound to haptocorrin and transcobalamin. The [57Co] radioactivity bound to haptocorrin and transcobalamin was determined after separating the proteins by adsorption to insolubilized antibodies to haptocorrin. The distribution of the radioactivity on the different forms of cobalamin and on haptocorrin isoproteins were determined. The fractional catabolic rate of hapatocorrin (0.102 to 0.158 d-1) and transcobalamin (3 to 29 d-1) was of the same magnitude as previously reported for a reference population. During the first 24 hours of the turnover, no change of the cyanocobalamin form was registered, indicating that conversion of cyanocobalamin to the coenzyme forms of cobalamin does not take place in the circulation. The haptocorrin isoprotein pattern changes in alkaline direction during the first 5 hours indicated that the glycoprotein was desialinated during circulation. From the calculated turnover parameter, the possible competition between transcobalamin and haptocorrin for the transport of cobalamins in CML is estimated to be of minor clinical significance. In conclusion, the increased plasma concentration of haptocorrin in CML is due to an increased liberation of haptocorrin and is not due to a decreased turnover of the protein. SN - 0022-2143 UR - https://www.unboundmedicine.com/medline/citation/8301203/The_turnover_of_57Co_labeled_cyanocobalamin_bound_to_cobalamin_binding_proteins_in_patients_with_chronic_myelogenous_leukemia_ L2 - https://medlineplus.gov/chronicmyeloidleukemia.html DB - PRIME DP - Unbound Medicine ER -