Tags

Type your tag names separated by a space and hit enter

Identification and characterization of a mobilization gene in the streptococcal plasmid, pVA380-1.
Plasmid 1993; 30(3):296-302P

Abstract

The 4.2-kb cryptic streptococcal plasmid, pVA380-1, has a broad host range and replicates via a rolling circle mode. The nucleotide base sequence of the 2.5-kb basic replicon of this plasmid, containing a plus origin, a minus origin, and a rep gene, was reported previously. The completed nucleotide base sequence of pVA380-1 revealed the presence of two additional open reading frames, ORF2 and ORF3. The protein predicted by ORF2 shared 74% amino acid identity with the Mob protein of the Group B streptococcal plasmid, pMV158. The 170 bases immediately 5' to ORF2 and to mob of pMV158 shared 87% homology and included a putative -10 region and a RSa site. A derivative of pVA380-1 into which a kanamycin resistance gene was inserted outside of the above 170 base region and ORF2 was mobilized by the conjugative streptococcal plasmid, pAM beta 1, into Enterococcus faecalis and Streptococcus sobrinus. Interruption of ORF2 by the same kanamycin resistance gene abolished the mobilizability of pVA380-1.

Authors+Show Affiliations

Department of Microbiology, University of Texas Health Science Center at San Antonio 78284.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8302939

Citation

LeBlanc, D J., et al. "Identification and Characterization of a Mobilization Gene in the Streptococcal Plasmid, PVA380-1." Plasmid, vol. 30, no. 3, 1993, pp. 296-302.
LeBlanc DJ, Chen YY, Lee LN. Identification and characterization of a mobilization gene in the streptococcal plasmid, pVA380-1. Plasmid. 1993;30(3):296-302.
LeBlanc, D. J., Chen, Y. Y., & Lee, L. N. (1993). Identification and characterization of a mobilization gene in the streptococcal plasmid, pVA380-1. Plasmid, 30(3), pp. 296-302.
LeBlanc DJ, Chen YY, Lee LN. Identification and Characterization of a Mobilization Gene in the Streptococcal Plasmid, PVA380-1. Plasmid. 1993;30(3):296-302. PubMed PMID: 8302939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of a mobilization gene in the streptococcal plasmid, pVA380-1. AU - LeBlanc,D J, AU - Chen,Y Y, AU - Lee,L N, PY - 1993/11/1/pubmed PY - 1993/11/1/medline PY - 1993/11/1/entrez SP - 296 EP - 302 JF - Plasmid JO - Plasmid VL - 30 IS - 3 N2 - The 4.2-kb cryptic streptococcal plasmid, pVA380-1, has a broad host range and replicates via a rolling circle mode. The nucleotide base sequence of the 2.5-kb basic replicon of this plasmid, containing a plus origin, a minus origin, and a rep gene, was reported previously. The completed nucleotide base sequence of pVA380-1 revealed the presence of two additional open reading frames, ORF2 and ORF3. The protein predicted by ORF2 shared 74% amino acid identity with the Mob protein of the Group B streptococcal plasmid, pMV158. The 170 bases immediately 5' to ORF2 and to mob of pMV158 shared 87% homology and included a putative -10 region and a RSa site. A derivative of pVA380-1 into which a kanamycin resistance gene was inserted outside of the above 170 base region and ORF2 was mobilized by the conjugative streptococcal plasmid, pAM beta 1, into Enterococcus faecalis and Streptococcus sobrinus. Interruption of ORF2 by the same kanamycin resistance gene abolished the mobilizability of pVA380-1. SN - 0147-619X UR - https://www.unboundmedicine.com/medline/citation/8302939/Identification_and_characterization_of_a_mobilization_gene_in_the_streptococcal_plasmid_pVA380_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-619X(83)71063-2 DB - PRIME DP - Unbound Medicine ER -