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SecD and SecF facilitate protein export in Escherichia coli.
EMBO J 1994; 13(3):554-61EJ

Abstract

We show here that the rate of protein translocation in the bacterium Escherichia coli depends on the levels of the SecD and SecF proteins in the cell. Overexpression of SecD and SecF stimulates translocation in wild type cells and improves export of proteins with mutant signal sequences. Depletion of SecD and SecF from the cell greatly reduces but does not abolish protein translocation. A secDF::kan null mutant deleted for the genes encoding both proteins is cold-sensitive for growth and protein export, has a severe export defect at 37 degrees C and is barely viable. The phenotypes of a secD null mutant and a secF null mutant are identical to the secDF::kan double null mutant. These results partially resolve the conflict between genetic studies and results from in vitro translocation systems which do not require SecD and SecF for activity, affirm the importance of these proteins to the export process, and suggest that SecD and SecF function together to stimulate protein export in a role fundamentally different from other Sec proteins. Our results provide additional support for the notion that an early step in protein export is cold-sensitive.

Authors+Show Affiliations

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8313900

Citation

Pogliano, J A., and J Beckwith. "SecD and SecF Facilitate Protein Export in Escherichia Coli." The EMBO Journal, vol. 13, no. 3, 1994, pp. 554-61.
Pogliano JA, Beckwith J. SecD and SecF facilitate protein export in Escherichia coli. EMBO J. 1994;13(3):554-61.
Pogliano, J. A., & Beckwith, J. (1994). SecD and SecF facilitate protein export in Escherichia coli. The EMBO Journal, 13(3), pp. 554-61.
Pogliano JA, Beckwith J. SecD and SecF Facilitate Protein Export in Escherichia Coli. EMBO J. 1994 Feb 1;13(3):554-61. PubMed PMID: 8313900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SecD and SecF facilitate protein export in Escherichia coli. AU - Pogliano,J A, AU - Beckwith,J, PY - 1994/2/1/pubmed PY - 1994/2/1/medline PY - 1994/2/1/entrez SP - 554 EP - 61 JF - The EMBO journal JO - EMBO J. VL - 13 IS - 3 N2 - We show here that the rate of protein translocation in the bacterium Escherichia coli depends on the levels of the SecD and SecF proteins in the cell. Overexpression of SecD and SecF stimulates translocation in wild type cells and improves export of proteins with mutant signal sequences. Depletion of SecD and SecF from the cell greatly reduces but does not abolish protein translocation. A secDF::kan null mutant deleted for the genes encoding both proteins is cold-sensitive for growth and protein export, has a severe export defect at 37 degrees C and is barely viable. The phenotypes of a secD null mutant and a secF null mutant are identical to the secDF::kan double null mutant. These results partially resolve the conflict between genetic studies and results from in vitro translocation systems which do not require SecD and SecF for activity, affirm the importance of these proteins to the export process, and suggest that SecD and SecF function together to stimulate protein export in a role fundamentally different from other Sec proteins. Our results provide additional support for the notion that an early step in protein export is cold-sensitive. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/8313900/SecD_and_SecF_facilitate_protein_export_in_Escherichia_coli_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/8313900/ DB - PRIME DP - Unbound Medicine ER -