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Antigen dependent release of interleukin 5 in vitro from spleen cells of mice infected with Angiostrongylus cantonensis.
Int J Parasitol. 1993 Nov; 23(7):865-9.IJ

Abstract

The kinetics of eosinophil growth and/or survival stimulating factors (Eo-stimulating factors) released by spleen cells from A. cantonensis-infected mice were assessed by in vitro marrow cultures. When spleen cells from C57BL/6 mice 8 to 20 days p.i. were cultured with young adult A. cantonensis worm antigen or Con A, almost equal amounts of Eo-stimulating factors were detected in the conditioned media obtained from both stimulations. No Eo-stimulating factor activity was detected in cultures from spleen cells without stimulation or from normal spleen cells with stimulation. Production of Eo-stimulating factors was inhibited by the pretreatment of the spleen cells with anti-Thy1.2 or anti-L3T4 antibodies plus complement but not with anti-Lyt2.2 antibody. In the presence of anti-mouse IL-5 monoclonal antibody, the activity of Eo-stimulating factors was inhibited by up to 99%. IL-5, therefore, appears to play a principal role in induction of eosinophilia in mice infected with A. cantonensis.

Authors+Show Affiliations

Department of Parasitology, Akita University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8314370

Citation

Sugaya, H, et al. "Antigen Dependent Release of Interleukin 5 in Vitro From Spleen Cells of Mice Infected With Angiostrongylus Cantonensis." International Journal for Parasitology, vol. 23, no. 7, 1993, pp. 865-9.
Sugaya H, Abe T, Yoshimura K, et al. Antigen dependent release of interleukin 5 in vitro from spleen cells of mice infected with Angiostrongylus cantonensis. Int J Parasitol. 1993;23(7):865-9.
Sugaya, H., Abe, T., Yoshimura, K., & Sasaki, O. (1993). Antigen dependent release of interleukin 5 in vitro from spleen cells of mice infected with Angiostrongylus cantonensis. International Journal for Parasitology, 23(7), 865-9.
Sugaya H, et al. Antigen Dependent Release of Interleukin 5 in Vitro From Spleen Cells of Mice Infected With Angiostrongylus Cantonensis. Int J Parasitol. 1993;23(7):865-9. PubMed PMID: 8314370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigen dependent release of interleukin 5 in vitro from spleen cells of mice infected with Angiostrongylus cantonensis. AU - Sugaya,H, AU - Abe,T, AU - Yoshimura,K, AU - Sasaki,O, PY - 1993/11/1/pubmed PY - 1993/11/1/medline PY - 1993/11/1/entrez SP - 865 EP - 9 JF - International journal for parasitology JO - Int J Parasitol VL - 23 IS - 7 N2 - The kinetics of eosinophil growth and/or survival stimulating factors (Eo-stimulating factors) released by spleen cells from A. cantonensis-infected mice were assessed by in vitro marrow cultures. When spleen cells from C57BL/6 mice 8 to 20 days p.i. were cultured with young adult A. cantonensis worm antigen or Con A, almost equal amounts of Eo-stimulating factors were detected in the conditioned media obtained from both stimulations. No Eo-stimulating factor activity was detected in cultures from spleen cells without stimulation or from normal spleen cells with stimulation. Production of Eo-stimulating factors was inhibited by the pretreatment of the spleen cells with anti-Thy1.2 or anti-L3T4 antibodies plus complement but not with anti-Lyt2.2 antibody. In the presence of anti-mouse IL-5 monoclonal antibody, the activity of Eo-stimulating factors was inhibited by up to 99%. IL-5, therefore, appears to play a principal role in induction of eosinophilia in mice infected with A. cantonensis. SN - 0020-7519 UR - https://www.unboundmedicine.com/medline/citation/8314370/Antigen_dependent_release_of_interleukin_5_in_vitro_from_spleen_cells_of_mice_infected_with_Angiostrongylus_cantonensis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0020-7519(93)90051-Y DB - PRIME DP - Unbound Medicine ER -