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Membrane disordering effect of ethanol on cerebral microvessels of aged rats: a brief report.
Neurobiol Aging. 1993 May-Jun; 14(3):229-32.NA

Abstract

To determine if the membrane disordering effect of ethanol on cerebral microvessels is altered with age, the steady state fluorescence polarization (P) of diphenyl-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) which report on the membrane core and surface region, respectively, was determined in isolated cerebral microvessel membranes from 3-month-old (young) and 24-month-old (aged) male Fischer 344 rats in the absence or presence of 0, 25, 50, 100, 200, and 400 mM ethanol. The addition of ethanol resulted in concentration dependent decrease in DPH fluorescence polarization in both young and aged rat cerebral microvessels. Analysis of variance indicated that these two age groups did not differ from each other in their response to ethanol over this concentration range or in their baseline P. However, the TMA-DPH fluorescence polarization in cerebral microvessels in the absence or presence of various concentrations of ethanol was significantly lower in aged rats (p < 0.01). It is concluded that aging in rats is associated with a decreased membrane lipid order near the membrane surface of cerebral microvessels as assessed by TMA-DPH. Membrane disordering effect of ethanol on cerebral microvessels does not appear to be altered significantly with age.

Authors+Show Affiliations

St. Louis Department of Veterans Affairs Medical Center, MO.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

8321390

Citation

Mooradian, A D., and T L. Smith. "Membrane Disordering Effect of Ethanol On Cerebral Microvessels of Aged Rats: a Brief Report." Neurobiology of Aging, vol. 14, no. 3, 1993, pp. 229-32.
Mooradian AD, Smith TL. Membrane disordering effect of ethanol on cerebral microvessels of aged rats: a brief report. Neurobiol Aging. 1993;14(3):229-32.
Mooradian, A. D., & Smith, T. L. (1993). Membrane disordering effect of ethanol on cerebral microvessels of aged rats: a brief report. Neurobiology of Aging, 14(3), 229-32.
Mooradian AD, Smith TL. Membrane Disordering Effect of Ethanol On Cerebral Microvessels of Aged Rats: a Brief Report. Neurobiol Aging. 1993 May-Jun;14(3):229-32. PubMed PMID: 8321390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Membrane disordering effect of ethanol on cerebral microvessels of aged rats: a brief report. AU - Mooradian,A D, AU - Smith,T L, PY - 1993/5/1/pubmed PY - 1993/5/1/medline PY - 1993/5/1/entrez SP - 229 EP - 32 JF - Neurobiology of aging JO - Neurobiol Aging VL - 14 IS - 3 N2 - To determine if the membrane disordering effect of ethanol on cerebral microvessels is altered with age, the steady state fluorescence polarization (P) of diphenyl-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) which report on the membrane core and surface region, respectively, was determined in isolated cerebral microvessel membranes from 3-month-old (young) and 24-month-old (aged) male Fischer 344 rats in the absence or presence of 0, 25, 50, 100, 200, and 400 mM ethanol. The addition of ethanol resulted in concentration dependent decrease in DPH fluorescence polarization in both young and aged rat cerebral microvessels. Analysis of variance indicated that these two age groups did not differ from each other in their response to ethanol over this concentration range or in their baseline P. However, the TMA-DPH fluorescence polarization in cerebral microvessels in the absence or presence of various concentrations of ethanol was significantly lower in aged rats (p < 0.01). It is concluded that aging in rats is associated with a decreased membrane lipid order near the membrane surface of cerebral microvessels as assessed by TMA-DPH. Membrane disordering effect of ethanol on cerebral microvessels does not appear to be altered significantly with age. SN - 0197-4580 UR - https://www.unboundmedicine.com/medline/citation/8321390/Membrane_disordering_effect_of_ethanol_on_cerebral_microvessels_of_aged_rats:_a_brief_report_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0197-4580(93)90005-V DB - PRIME DP - Unbound Medicine ER -