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Involvement of the L-arginine: nitric oxide pathway in nonadrenergic noncholinergic relaxation of the cat gastric fundus.
J Pharmacol Exp Ther. 1993 Jul; 266(1):172-8.JP

Abstract

Vasoactive intestinal polypeptide (VIP) is a neurotransmitter of nonadrenergic noncholinergic (NANC) nerves in the cat gastric fundus. A possible additional role for nitric oxide (NO) was investigated in circular and longitudinal muscle strips from this tissue. Incubation with the inhibitor of NO-synthesis, NG-nitro-L-arginine methyl ester (L-NAME, 3 x 10(-4) M), inhibited the relaxant response to both short- and long-lasting electrical field stimulation. The substrate for NO synthesis, L-arginine (2 x 10(-3) M), prevented this inhibition. In experiments with long-lasting electrical field stimulation, trypsin (3 x 10(-6) M) and L-NAME inhibited the beginning of the NANC relaxation to the same extent, but the inhibition by trypsin was more pronounced at the end of the stimulation period. The inhibitory effect of L-NAME and trypsin was additive. L-NAME did not influence the relaxations induced by VIP (10(-7) M), NO (10(-5) M) and isoprenaline (3 x 10(-6) M). NG-nitro-L-arginine (3 x 10(-4) M) also did not change the response to VIP. The relaxation induced by 10(-5) M NO was not transient but was sustained. The plateau phase of this relaxation was reduced by trypsin but not by 3 x 10(-6) M tetrodotoxin. It is concluded that NO is involved in NANC relaxation of the cat gastric fundus. During sustained NANC relaxation in the cat gastric fundus, cotransmission of NO and VIP is possible.

Authors+Show Affiliations

Heymans Institute of Pharmacology, University of Gent Medical School, Belgium.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8331556

Citation

Barbier, A J., and R A. Lefebvre. "Involvement of the L-arginine: Nitric Oxide Pathway in Nonadrenergic Noncholinergic Relaxation of the Cat Gastric Fundus." The Journal of Pharmacology and Experimental Therapeutics, vol. 266, no. 1, 1993, pp. 172-8.
Barbier AJ, Lefebvre RA. Involvement of the L-arginine: nitric oxide pathway in nonadrenergic noncholinergic relaxation of the cat gastric fundus. J Pharmacol Exp Ther. 1993;266(1):172-8.
Barbier, A. J., & Lefebvre, R. A. (1993). Involvement of the L-arginine: nitric oxide pathway in nonadrenergic noncholinergic relaxation of the cat gastric fundus. The Journal of Pharmacology and Experimental Therapeutics, 266(1), 172-8.
Barbier AJ, Lefebvre RA. Involvement of the L-arginine: Nitric Oxide Pathway in Nonadrenergic Noncholinergic Relaxation of the Cat Gastric Fundus. J Pharmacol Exp Ther. 1993;266(1):172-8. PubMed PMID: 8331556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of the L-arginine: nitric oxide pathway in nonadrenergic noncholinergic relaxation of the cat gastric fundus. AU - Barbier,A J, AU - Lefebvre,R A, PY - 1993/7/1/pubmed PY - 1993/7/1/medline PY - 1993/7/1/entrez SP - 172 EP - 8 JF - The Journal of pharmacology and experimental therapeutics JO - J. Pharmacol. Exp. Ther. VL - 266 IS - 1 N2 - Vasoactive intestinal polypeptide (VIP) is a neurotransmitter of nonadrenergic noncholinergic (NANC) nerves in the cat gastric fundus. A possible additional role for nitric oxide (NO) was investigated in circular and longitudinal muscle strips from this tissue. Incubation with the inhibitor of NO-synthesis, NG-nitro-L-arginine methyl ester (L-NAME, 3 x 10(-4) M), inhibited the relaxant response to both short- and long-lasting electrical field stimulation. The substrate for NO synthesis, L-arginine (2 x 10(-3) M), prevented this inhibition. In experiments with long-lasting electrical field stimulation, trypsin (3 x 10(-6) M) and L-NAME inhibited the beginning of the NANC relaxation to the same extent, but the inhibition by trypsin was more pronounced at the end of the stimulation period. The inhibitory effect of L-NAME and trypsin was additive. L-NAME did not influence the relaxations induced by VIP (10(-7) M), NO (10(-5) M) and isoprenaline (3 x 10(-6) M). NG-nitro-L-arginine (3 x 10(-4) M) also did not change the response to VIP. The relaxation induced by 10(-5) M NO was not transient but was sustained. The plateau phase of this relaxation was reduced by trypsin but not by 3 x 10(-6) M tetrodotoxin. It is concluded that NO is involved in NANC relaxation of the cat gastric fundus. During sustained NANC relaxation in the cat gastric fundus, cotransmission of NO and VIP is possible. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/8331556/Involvement_of_the_L_arginine:_nitric_oxide_pathway_in_nonadrenergic_noncholinergic_relaxation_of_the_cat_gastric_fundus_ L2 - http://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=8331556 DB - PRIME DP - Unbound Medicine ER -