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Sodium nitroprusside stimulates anion secretion and inhibits sodium chloride absorption in rat colon.
J Pharmacol Exp Ther. 1993 Jul; 266(1):224-30.JP

Abstract

The effects of sodium nitroprusside (SNP) on intestinal electrolyte transport were studied in rat colon mounted in Ussing chambers. Serosal addition of SNP increases short-circuit current (Isc) in a concentration-dependent manner. The maximal change in Isc was 35.6 +/- 2.3 microA/cm2 at 1 mM SNP (ED50 approximately 81 microM). S-nitroso-N-acetylpenicillamine, a nitrosothiol which can release nitric oxide, also stimulated an increase in Isc of 13.9 +/- 3.1 microA/cm2 at a concentration of 100 microM. The response to SNP was rapid, peaking at about 4 min with partial return to baseline at 20 to 30 min. Isc responses to SNP were significantly less in the cecum and ileum as well as after mucosal addition in the distal colon. Pretreatment with serosal atropine, cimetidine, pyrilamine, ketanserin, and N omega-nitro-L-arginine and mucosal amiloride did not inhibit the SNP-stimulated Isc; iodine, methylene blue, bumetanide, piroxicam, and tetrodotoxin each significantly decreased the response and piroxicam plus tetrodotoxin abolished it. Transmural 22Na+ and 36Cl- flux studies demonstrated that the change in Isc produced by SNP was attributable to anion secretion. SNP also inhibited neutral Na+ and Cl- absorption, with the inhibition of Na+, but not Cl- absorption eliminated by tetrodotoxin and piroxicam pretreatment. In summary, SNP has several effects on intestinal electrolyte transport, suggesting that nitric oxide or other components of nitrovasodilators may be important physiological mediators of salt and water transport and may play a role in stimulated colonic epithelial electrolyte transport in inflamed tissues.

Authors+Show Affiliations

Department of Medicine, University of Chicago, Illinois.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8331560

Citation

Wilson, K T., et al. "Sodium Nitroprusside Stimulates Anion Secretion and Inhibits Sodium Chloride Absorption in Rat Colon." The Journal of Pharmacology and Experimental Therapeutics, vol. 266, no. 1, 1993, pp. 224-30.
Wilson KT, Xie Y, Musch MW, et al. Sodium nitroprusside stimulates anion secretion and inhibits sodium chloride absorption in rat colon. J Pharmacol Exp Ther. 1993;266(1):224-30.
Wilson, K. T., Xie, Y., Musch, M. W., & Chang, E. B. (1993). Sodium nitroprusside stimulates anion secretion and inhibits sodium chloride absorption in rat colon. The Journal of Pharmacology and Experimental Therapeutics, 266(1), 224-30.
Wilson KT, et al. Sodium Nitroprusside Stimulates Anion Secretion and Inhibits Sodium Chloride Absorption in Rat Colon. J Pharmacol Exp Ther. 1993;266(1):224-30. PubMed PMID: 8331560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sodium nitroprusside stimulates anion secretion and inhibits sodium chloride absorption in rat colon. AU - Wilson,K T, AU - Xie,Y, AU - Musch,M W, AU - Chang,E B, PY - 1993/7/1/pubmed PY - 1993/7/1/medline PY - 1993/7/1/entrez SP - 224 EP - 30 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 266 IS - 1 N2 - The effects of sodium nitroprusside (SNP) on intestinal electrolyte transport were studied in rat colon mounted in Ussing chambers. Serosal addition of SNP increases short-circuit current (Isc) in a concentration-dependent manner. The maximal change in Isc was 35.6 +/- 2.3 microA/cm2 at 1 mM SNP (ED50 approximately 81 microM). S-nitroso-N-acetylpenicillamine, a nitrosothiol which can release nitric oxide, also stimulated an increase in Isc of 13.9 +/- 3.1 microA/cm2 at a concentration of 100 microM. The response to SNP was rapid, peaking at about 4 min with partial return to baseline at 20 to 30 min. Isc responses to SNP were significantly less in the cecum and ileum as well as after mucosal addition in the distal colon. Pretreatment with serosal atropine, cimetidine, pyrilamine, ketanserin, and N omega-nitro-L-arginine and mucosal amiloride did not inhibit the SNP-stimulated Isc; iodine, methylene blue, bumetanide, piroxicam, and tetrodotoxin each significantly decreased the response and piroxicam plus tetrodotoxin abolished it. Transmural 22Na+ and 36Cl- flux studies demonstrated that the change in Isc produced by SNP was attributable to anion secretion. SNP also inhibited neutral Na+ and Cl- absorption, with the inhibition of Na+, but not Cl- absorption eliminated by tetrodotoxin and piroxicam pretreatment. In summary, SNP has several effects on intestinal electrolyte transport, suggesting that nitric oxide or other components of nitrovasodilators may be important physiological mediators of salt and water transport and may play a role in stimulated colonic epithelial electrolyte transport in inflamed tissues. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/8331560/Sodium_nitroprusside_stimulates_anion_secretion_and_inhibits_sodium_chloride_absorption_in_rat_colon_ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=8331560 DB - PRIME DP - Unbound Medicine ER -