Tags

Type your tag names separated by a space and hit enter

IFN-gamma inhibits the replication of Listeria monocytogenes in hepatocytes.
J Immunol. 1993 Aug 01; 151(3):1401-9.JI

Abstract

We previously reported that the bulk of Listeria monocytogenes injected intravenously into mice is taken up in the liver and replicates within the parenchymal cells (hepatocytes). Although IFN-gamma is known to play an important role in host defenses to listerial infections of the liver, the mechanism(s) that underlies this role remains to be fully delineated. In the initial experiments presented here, we demonstrated the elevated expression of IFN-gamma message in the livers of mice during primary listerial infections. Subsequent experiments showed that the listerial burden of the hepatocyte population was increased significantly in mice administered monoclonal anti-IFN-gamma. Conversely, the administration of murine rIFN-gamma resulted in a marked (2 log10) decrease in the number of hepatocyte-associated Listeria. In vitro, IFN-gamma stimulated the listericidal activity of purified hepatocytes. Infected hepatocytes incubated in the presence of > 0.1 U/ml murine rIFN-gamma exhibited a significant reduction in intracellular Listeria. The elevated antilisterial activity of IFN-gamma-treated hepatocytes in culture was abrogated by the presence of compounds that scavenged or inhibited the production of reactive oxygen intermediates. Taken together, these findings suggest that activation of the oxygen-dependent, antimicrobial activity of hepatocytes may constitute a principal effector function of IFN-gamma in host defenses to listerial infections of the liver.

Authors+Show Affiliations

Department of Medicine, University of Pittsburgh School of Medicine, Montefiore University Hospital, PA 15213.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8335936

Citation

Gregory, S H., and E J. Wing. "IFN-gamma Inhibits the Replication of Listeria Monocytogenes in Hepatocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 151, no. 3, 1993, pp. 1401-9.
Gregory SH, Wing EJ. IFN-gamma inhibits the replication of Listeria monocytogenes in hepatocytes. J Immunol. 1993;151(3):1401-9.
Gregory, S. H., & Wing, E. J. (1993). IFN-gamma inhibits the replication of Listeria monocytogenes in hepatocytes. Journal of Immunology (Baltimore, Md. : 1950), 151(3), 1401-9.
Gregory SH, Wing EJ. IFN-gamma Inhibits the Replication of Listeria Monocytogenes in Hepatocytes. J Immunol. 1993 Aug 1;151(3):1401-9. PubMed PMID: 8335936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IFN-gamma inhibits the replication of Listeria monocytogenes in hepatocytes. AU - Gregory,S H, AU - Wing,E J, PY - 1993/8/1/pubmed PY - 1993/8/1/medline PY - 1993/8/1/entrez SP - 1401 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 151 IS - 3 N2 - We previously reported that the bulk of Listeria monocytogenes injected intravenously into mice is taken up in the liver and replicates within the parenchymal cells (hepatocytes). Although IFN-gamma is known to play an important role in host defenses to listerial infections of the liver, the mechanism(s) that underlies this role remains to be fully delineated. In the initial experiments presented here, we demonstrated the elevated expression of IFN-gamma message in the livers of mice during primary listerial infections. Subsequent experiments showed that the listerial burden of the hepatocyte population was increased significantly in mice administered monoclonal anti-IFN-gamma. Conversely, the administration of murine rIFN-gamma resulted in a marked (2 log10) decrease in the number of hepatocyte-associated Listeria. In vitro, IFN-gamma stimulated the listericidal activity of purified hepatocytes. Infected hepatocytes incubated in the presence of > 0.1 U/ml murine rIFN-gamma exhibited a significant reduction in intracellular Listeria. The elevated antilisterial activity of IFN-gamma-treated hepatocytes in culture was abrogated by the presence of compounds that scavenged or inhibited the production of reactive oxygen intermediates. Taken together, these findings suggest that activation of the oxygen-dependent, antimicrobial activity of hepatocytes may constitute a principal effector function of IFN-gamma in host defenses to listerial infections of the liver. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8335936/IFN_gamma_inhibits_the_replication_of_Listeria_monocytogenes_in_hepatocytes_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=8335936 DB - PRIME DP - Unbound Medicine ER -