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Chimeric EWS-FLI1 transcript in a Ewing cell line with a complex t(11;22;14) translocation.
Cancer Res. 1993 Aug 15; 53(16):3655-7.CR

Abstract

Peripheral neuroectodermal tumors include the differentiated neuroepithelioma and the undifferentiated Ewing's tumor. Despite clinical and pathological differences, both malignancies are characterized by a t(11;22)(q24;q12) translocation which is observed in > 90% of the cases. Molecularly, the translocation is underlaid by a rearrangement between the EWS and Fli-1 genes on chromosomes 22 and 11, respectively. Because of the difficulties in the differential diagnosis between various small round cell tumors of childhood, including Ewing's tumor, a molecular diagnostic assay would be desirable. A prerequisite for predicting the reliability of such a test resides in the molecular elucidation of the peripheral neuroectodermal tumor cases which do not exhibit the prototypical translocation. We have analyzed one such case of Ewing's tumor-derived cell line with a t(11;22;14)(q24;q12;q11) translocation. An EWS-Fli-1 fusion transcript was evidenced by polymerase chain reaction amplification of a reverse transcription product obtained from total RNA. Direct sequencing was performed to demonstrate that the molecular rearrangement in this particular Ewing sample resulted in a fusion transcript similar to those observed in tumors with the prototypical translocation.

Authors+Show Affiliations

Laboratoire de Biologie des Tumeurs Humaines, CNRS URA 1156, Institut Gustave Roussy, Villejuif, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8339272

Citation

Bonin, G, et al. "Chimeric EWS-FLI1 Transcript in a Ewing Cell Line With a Complex T(11;22;14) Translocation." Cancer Research, vol. 53, no. 16, 1993, pp. 3655-7.
Bonin G, Scamps C, Turc-Carel C, et al. Chimeric EWS-FLI1 transcript in a Ewing cell line with a complex t(11;22;14) translocation. Cancer Res. 1993;53(16):3655-7.
Bonin, G., Scamps, C., Turc-Carel, C., & Lipinski, M. (1993). Chimeric EWS-FLI1 transcript in a Ewing cell line with a complex t(11;22;14) translocation. Cancer Research, 53(16), 3655-7.
Bonin G, et al. Chimeric EWS-FLI1 Transcript in a Ewing Cell Line With a Complex T(11;22;14) Translocation. Cancer Res. 1993 Aug 15;53(16):3655-7. PubMed PMID: 8339272.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chimeric EWS-FLI1 transcript in a Ewing cell line with a complex t(11;22;14) translocation. AU - Bonin,G, AU - Scamps,C, AU - Turc-Carel,C, AU - Lipinski,M, PY - 1993/8/15/pubmed PY - 1993/8/15/medline PY - 1993/8/15/entrez SP - 3655 EP - 7 JF - Cancer research JO - Cancer Res VL - 53 IS - 16 N2 - Peripheral neuroectodermal tumors include the differentiated neuroepithelioma and the undifferentiated Ewing's tumor. Despite clinical and pathological differences, both malignancies are characterized by a t(11;22)(q24;q12) translocation which is observed in > 90% of the cases. Molecularly, the translocation is underlaid by a rearrangement between the EWS and Fli-1 genes on chromosomes 22 and 11, respectively. Because of the difficulties in the differential diagnosis between various small round cell tumors of childhood, including Ewing's tumor, a molecular diagnostic assay would be desirable. A prerequisite for predicting the reliability of such a test resides in the molecular elucidation of the peripheral neuroectodermal tumor cases which do not exhibit the prototypical translocation. We have analyzed one such case of Ewing's tumor-derived cell line with a t(11;22;14)(q24;q12;q11) translocation. An EWS-Fli-1 fusion transcript was evidenced by polymerase chain reaction amplification of a reverse transcription product obtained from total RNA. Direct sequencing was performed to demonstrate that the molecular rearrangement in this particular Ewing sample resulted in a fusion transcript similar to those observed in tumors with the prototypical translocation. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/8339272/Chimeric_EWS_FLI1_transcript_in_a_Ewing_cell_line_with_a_complex_t_11 L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=8339272 DB - PRIME DP - Unbound Medicine ER -