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Cell-surface expression of human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain (CD74) does not always correlate with cell-surface expression of HLA class II molecules.
Immunology. 1993 Jun; 79(2):331-5.I

Abstract

The human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain comprises at least four polypeptides. One of these is expressed as a membrane-bound subunit and has been designated as CD74. We investigated the expression of CD74 on B- and T-cell types utilizing monoclonal antibodies (mAb) specific for the c-terminus of CD74. All B cells tested expressed CD74 regardless of their HLA phenotypes. High and low CD74 expressors were provisionally assigned based on quantitative variations found in some haplotypes. A mutant cell with selective deletions of class II loci DR and DQ but with retention of DP and additional class II heterodimers synthesize normal amounts of CD74. In contrast, however, a mutant with a total class II deletion had diminished expression of CD74, suggesting that at least one of the class II isotypes is essential for full constitutive expression of CD74. Major histocompatibility complex (MHC) class II negative T-cell lines and CD3+ T cell isolated from peripheral blood did not express CD74. However, allostimulated T cells which express de novo induced class II determinants also did not express CD74. Taken together, these results show that CD74 is expressed on B cells and unexpectedly is not synthesized on de novo-induced class II positive T-cell clones.

Authors+Show Affiliations

Department of Biology, University of Essex, Colchester, U.K.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8344710

Citation

Wilson, K M., et al. "Cell-surface Expression of Human Histocompatibility Leucocyte Antigen (HLA) Class II-associated Invariant Chain (CD74) Does Not Always Correlate With Cell-surface Expression of HLA Class II Molecules." Immunology, vol. 79, no. 2, 1993, pp. 331-5.
Wilson KM, Labeta MO, Pawelec G, et al. Cell-surface expression of human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain (CD74) does not always correlate with cell-surface expression of HLA class II molecules. Immunology. 1993;79(2):331-5.
Wilson, K. M., Labeta, M. O., Pawelec, G., & Fernandez, N. (1993). Cell-surface expression of human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain (CD74) does not always correlate with cell-surface expression of HLA class II molecules. Immunology, 79(2), 331-5.
Wilson KM, et al. Cell-surface Expression of Human Histocompatibility Leucocyte Antigen (HLA) Class II-associated Invariant Chain (CD74) Does Not Always Correlate With Cell-surface Expression of HLA Class II Molecules. Immunology. 1993;79(2):331-5. PubMed PMID: 8344710.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell-surface expression of human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain (CD74) does not always correlate with cell-surface expression of HLA class II molecules. AU - Wilson,K M, AU - Labeta,M O, AU - Pawelec,G, AU - Fernandez,N, PY - 1993/6/1/pubmed PY - 1993/6/1/medline PY - 1993/6/1/entrez SP - 331 EP - 5 JF - Immunology JO - Immunology VL - 79 IS - 2 N2 - The human histocompatibility leucocyte antigen (HLA) class II-associated invariant chain comprises at least four polypeptides. One of these is expressed as a membrane-bound subunit and has been designated as CD74. We investigated the expression of CD74 on B- and T-cell types utilizing monoclonal antibodies (mAb) specific for the c-terminus of CD74. All B cells tested expressed CD74 regardless of their HLA phenotypes. High and low CD74 expressors were provisionally assigned based on quantitative variations found in some haplotypes. A mutant cell with selective deletions of class II loci DR and DQ but with retention of DP and additional class II heterodimers synthesize normal amounts of CD74. In contrast, however, a mutant with a total class II deletion had diminished expression of CD74, suggesting that at least one of the class II isotypes is essential for full constitutive expression of CD74. Major histocompatibility complex (MHC) class II negative T-cell lines and CD3+ T cell isolated from peripheral blood did not express CD74. However, allostimulated T cells which express de novo induced class II determinants also did not express CD74. Taken together, these results show that CD74 is expressed on B cells and unexpectedly is not synthesized on de novo-induced class II positive T-cell clones. SN - 0019-2805 UR - https://www.unboundmedicine.com/medline/citation/8344710/Cell_surface_expression_of_human_histocompatibility_leucocyte_antigen__HLA__class_II_associated_invariant_chain__CD74__does_not_always_correlate_with_cell_surface_expression_of_HLA_class_II_molecules_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/8344710/ DB - PRIME DP - Unbound Medicine ER -