Prime

Type your tag names separated by a space and hit enter

Penetration of oligonucleotides into mouse organism through mucosa and skin.

Abstract

Benzylamide 5'-32P-oligonucleotide derivatives were shown to penetrate into mice organism when administered by various routes; intranasally, per os, intravaginally and per rectum. In all cases, the compounds are rapidly accumulated in blood and guts. Analysis of the radioactive material from blood and pancreas revealed intact oligonucleotides. Although concentrations of oligonucleotides in tissues differ considerably by the various methods of administration, the efficiency of delivery is sufficient to consider all the routes as being of therapeutic value. Dose effect on the efficiency of oligonucleotide penetration into mice suggests the transport to be a saturable process. Application of an oligonucleotide lotion on mice ear helices results in reproducible accumulation of radioactivity in the animal tissues. Effectiveness of oligonucleotide delivery into mouse through skin can be improved by using electrophoretic procedure.

Links

  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk.

    ,

    Source

    FEBS letters 327:3 1993 Aug 2 pg 271-4

    MeSH

    Animals
    Base Sequence
    Drug Administration Routes
    Mice
    Mice, Inbred BALB C
    Molecular Sequence Data
    Mucous Membrane
    Oligodeoxyribonucleotides
    Phosphorus Radioisotopes
    Skin
    Skin Absorption
    Tissue Distribution

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    8348953

    Citation

    TY - JOUR T1 - Penetration of oligonucleotides into mouse organism through mucosa and skin. AU - Vlassov,V V, AU - Karamyshev,V N, AU - Yakubov,L A, PY - 1993/8/2/pubmed PY - 1993/8/2/medline PY - 1993/8/2/entrez SP - 271 EP - 4 JF - FEBS letters JO - FEBS Lett. VL - 327 IS - 3 N2 - Benzylamide 5'-32P-oligonucleotide derivatives were shown to penetrate into mice organism when administered by various routes; intranasally, per os, intravaginally and per rectum. In all cases, the compounds are rapidly accumulated in blood and guts. Analysis of the radioactive material from blood and pancreas revealed intact oligonucleotides. Although concentrations of oligonucleotides in tissues differ considerably by the various methods of administration, the efficiency of delivery is sufficient to consider all the routes as being of therapeutic value. Dose effect on the efficiency of oligonucleotide penetration into mice suggests the transport to be a saturable process. Application of an oligonucleotide lotion on mice ear helices results in reproducible accumulation of radioactivity in the animal tissues. Effectiveness of oligonucleotide delivery into mouse through skin can be improved by using electrophoretic procedure. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/8348953/Penetration_of_oligonucleotides_into_mouse_organism_through_mucosa_and_skin_ L2 - http://linkinghub.elsevier.com/retrieve/pii/0014-5793(93)81002-H ER -