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IL-2 up-regulates but IFN-gamma suppresses IL-8 expression in human monocytes.
J Immunol. 1993 Sep 01; 151(5):2725-32.JI

Abstract

IL-2 has pleiotropic properties and is a potent activator of monocytic functions. Since monocytes are an important source of the chemoattractant cytokine IL-8, we studied the effects of IL-2 on the expression of IL-8 in human monocytes. IL-8 mRNA expression was detectable in resting human monocytes. Treatment of monocytes with IL-2 increased IL-8 mRNA expression by a protein synthesis-independent process. The augmentation of IL-8 mRNA by IL-2 was associated with an increase in IL-8 secretion. The expression of IL-8 mRNA was not a nonspecific response to any stimulus of monocyte activation. In fact, IFN-gamma, which is also a potent monocyte activator, not only failed to induce IL-8 expression but inhibited the stimulation of IL-8 by IL-2. Nuclear run-on experiments demonstrated that both the enhancement of IL-8 mRNA expression and its down-regulation by IFN-gamma occurred at the transcriptional level. These results show for the first time that in fresh human monocytes, IL-8 expression is differentially regulated by IL-2 and IFN-gamma and suggest that the interactions among IL-2, IL-8, and IFN-gamma may be important for the development and control of the inflammatory response.

Authors+Show Affiliations

Program Resources, Inc/DynCorp., Frederick Cancer Research and Development Center, MD 21702.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8360487

Citation

Gusella, G L., et al. "IL-2 Up-regulates but IFN-gamma Suppresses IL-8 Expression in Human Monocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 151, no. 5, 1993, pp. 2725-32.
Gusella GL, Musso T, Bosco MC, et al. IL-2 up-regulates but IFN-gamma suppresses IL-8 expression in human monocytes. J Immunol. 1993;151(5):2725-32.
Gusella, G. L., Musso, T., Bosco, M. C., Espinoza-Delgado, I., Matsushima, K., & Varesio, L. (1993). IL-2 up-regulates but IFN-gamma suppresses IL-8 expression in human monocytes. Journal of Immunology (Baltimore, Md. : 1950), 151(5), 2725-32.
Gusella GL, et al. IL-2 Up-regulates but IFN-gamma Suppresses IL-8 Expression in Human Monocytes. J Immunol. 1993 Sep 1;151(5):2725-32. PubMed PMID: 8360487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-2 up-regulates but IFN-gamma suppresses IL-8 expression in human monocytes. AU - Gusella,G L, AU - Musso,T, AU - Bosco,M C, AU - Espinoza-Delgado,I, AU - Matsushima,K, AU - Varesio,L, PY - 1993/9/1/pubmed PY - 1993/9/1/medline PY - 1993/9/1/entrez SP - 2725 EP - 32 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 151 IS - 5 N2 - IL-2 has pleiotropic properties and is a potent activator of monocytic functions. Since monocytes are an important source of the chemoattractant cytokine IL-8, we studied the effects of IL-2 on the expression of IL-8 in human monocytes. IL-8 mRNA expression was detectable in resting human monocytes. Treatment of monocytes with IL-2 increased IL-8 mRNA expression by a protein synthesis-independent process. The augmentation of IL-8 mRNA by IL-2 was associated with an increase in IL-8 secretion. The expression of IL-8 mRNA was not a nonspecific response to any stimulus of monocyte activation. In fact, IFN-gamma, which is also a potent monocyte activator, not only failed to induce IL-8 expression but inhibited the stimulation of IL-8 by IL-2. Nuclear run-on experiments demonstrated that both the enhancement of IL-8 mRNA expression and its down-regulation by IFN-gamma occurred at the transcriptional level. These results show for the first time that in fresh human monocytes, IL-8 expression is differentially regulated by IL-2 and IFN-gamma and suggest that the interactions among IL-2, IL-8, and IFN-gamma may be important for the development and control of the inflammatory response. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/8360487/IL_2_up_regulates_but_IFN_gamma_suppresses_IL_8_expression_in_human_monocytes_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=8360487 DB - PRIME DP - Unbound Medicine ER -