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Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease.
Yeast. 1993 Jul; 9(7):771-82.Y

Abstract

The LYP1 gene of Saccharomyces cerevisiae was cloned by complementation in lysine-permease-deficient recipient yeast cells, and its nucleotide sequence was determined. An open reading frame of 1833 nucleotides was found encoding a polypeptide of 611 amino acids, with a calculated molecular weight of 68 118. Analysis of the deduced primary structure of the protein revealed ten membrane-spanning regions and three potential N-glycosylation sites. Analysis of the deduced sequence of protein LYP1 indicates homology with other yeast amino-acid permeases, in particular with CAN1, and also the lysine-specific permease of Escherichia coli. The strain transformed by a multi-copy plasmid harbouring the LYP1 gene, showed a 20-fold increase in the maximum velocity of lysine uptake over that in the wild type, with no changes in the affinity of the permease for its substrate.

Authors+Show Affiliations

Sychrova H
Institut de Biologie Moléculaire et Cellulaire, C.N.R.S., Strasbourg, France.
Chevallier MR
No affiliation info available

MeSH

Amino Acid SequenceAmino Acid Transport Systems, BasicBase SequenceBiological TransportCloning, MolecularDNA, FungalGenes, FungalKineticsMembrane Transport ProteinsMolecular Sequence DataRestriction MappingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino Acid

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8368011

Citation

Sychrova, H, and M R. Chevallier. "Cloning and Sequencing of the Saccharomyces Cerevisiae Gene LYP1 Coding for a Lysine-specific Permease." Yeast (Chichester, England), vol. 9, no. 7, 1993, pp. 771-82.
Sychrova H, Chevallier MR. Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease. Yeast. 1993;9(7):771-82.
Sychrova, H., & Chevallier, M. R. (1993). Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease. Yeast (Chichester, England), 9(7), 771-82.
Sychrova H, Chevallier MR. Cloning and Sequencing of the Saccharomyces Cerevisiae Gene LYP1 Coding for a Lysine-specific Permease. Yeast. 1993;9(7):771-82. PubMed PMID: 8368011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease. AU - Sychrova,H, AU - Chevallier,M R, PY - 1993/7/1/pubmed PY - 1993/7/1/medline PY - 1993/7/1/entrez SP - 771 EP - 82 JF - Yeast (Chichester, England) JO - Yeast VL - 9 IS - 7 N2 - The LYP1 gene of Saccharomyces cerevisiae was cloned by complementation in lysine-permease-deficient recipient yeast cells, and its nucleotide sequence was determined. An open reading frame of 1833 nucleotides was found encoding a polypeptide of 611 amino acids, with a calculated molecular weight of 68 118. Analysis of the deduced primary structure of the protein revealed ten membrane-spanning regions and three potential N-glycosylation sites. Analysis of the deduced sequence of protein LYP1 indicates homology with other yeast amino-acid permeases, in particular with CAN1, and also the lysine-specific permease of Escherichia coli. The strain transformed by a multi-copy plasmid harbouring the LYP1 gene, showed a 20-fold increase in the maximum velocity of lysine uptake over that in the wild type, with no changes in the affinity of the permease for its substrate. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/8368011/Cloning_and_sequencing_of_the_Saccharomyces_cerevisiae_gene_LYP1_coding_for_a_lysine_specific_permease_ L2 - https://doi.org/10.1002/yea.320090711 DB - PRIME DP - Unbound Medicine ER -