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Regulation of glycolytic enzymes and the Crabtree effect in galactose-limited continuous cultures of Saccharomyces cerevisiae.
Yeast. 1993 Jul; 9(7):787-95.Y

Abstract

In order to determine whether the changes in the activities and mRNA levels of enzymes involved in intermediary carbon metabolism previously observed in glucose-limited continuous cultures (Sierkstra et al., 1992a) were glucose specific, we have analysed their regulation in a galactose-limited continuous culture of Saccharomyces cerevisiae. The Vmax of the galactose uptake system was shown to be dilution rate (D) dependent, comparable with the high-affinity glucose uptake. The maximum uptake was observed at D 0.2 h-1 (0.25 mmol min-1 per g) and the minimum uptake (0.1 mmol min-1 per g) at D 0.05 h-1 and 0.3 h-1. The aerobic fermentation of galactose occurred at D 0.275-0.3 h-1 which is identical to the results obtained in glucose-limited continuous cultures of this strain. Because galactose is not a repressing carbon source, this demonstrates that the Crabtree effect is not mediated by, or in any way related to glucose repression. Moreover, invertase and hexokinase I mRNA levels (both subject to glucose repression at the transcriptional level) were present when the yeast produced ethanol in galactose- and glucose-limited continuous cultures. In glucose-limited continuous cultures a decrease in alcohol dehydrogenase (I and II) mRNA levels and activity and phosphoglucomutase activity was observed with increasing dilution rates. In addition, at D 0.3 h-1, when the yeast produced ethanol, glucose-6-phosphate dehydrogenase and pyruvate decarboxylase were induced and a decrease in respiration was observed.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Department of Molecular Cell Biology, University of Utrecht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8368013

Citation

Sierkstra, L N., et al. "Regulation of Glycolytic Enzymes and the Crabtree Effect in Galactose-limited Continuous Cultures of Saccharomyces Cerevisiae." Yeast (Chichester, England), vol. 9, no. 7, 1993, pp. 787-95.
Sierkstra LN, Nouwen NP, Verbakel JM, et al. Regulation of glycolytic enzymes and the Crabtree effect in galactose-limited continuous cultures of Saccharomyces cerevisiae. Yeast. 1993;9(7):787-95.
Sierkstra, L. N., Nouwen, N. P., Verbakel, J. M., & Verrips, C. T. (1993). Regulation of glycolytic enzymes and the Crabtree effect in galactose-limited continuous cultures of Saccharomyces cerevisiae. Yeast (Chichester, England), 9(7), 787-95.
Sierkstra LN, et al. Regulation of Glycolytic Enzymes and the Crabtree Effect in Galactose-limited Continuous Cultures of Saccharomyces Cerevisiae. Yeast. 1993;9(7):787-95. PubMed PMID: 8368013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of glycolytic enzymes and the Crabtree effect in galactose-limited continuous cultures of Saccharomyces cerevisiae. AU - Sierkstra,L N, AU - Nouwen,N P, AU - Verbakel,J M, AU - Verrips,C T, PY - 1993/7/1/pubmed PY - 1993/7/1/medline PY - 1993/7/1/entrez SP - 787 EP - 95 JF - Yeast (Chichester, England) JO - Yeast VL - 9 IS - 7 N2 - In order to determine whether the changes in the activities and mRNA levels of enzymes involved in intermediary carbon metabolism previously observed in glucose-limited continuous cultures (Sierkstra et al., 1992a) were glucose specific, we have analysed their regulation in a galactose-limited continuous culture of Saccharomyces cerevisiae. The Vmax of the galactose uptake system was shown to be dilution rate (D) dependent, comparable with the high-affinity glucose uptake. The maximum uptake was observed at D 0.2 h-1 (0.25 mmol min-1 per g) and the minimum uptake (0.1 mmol min-1 per g) at D 0.05 h-1 and 0.3 h-1. The aerobic fermentation of galactose occurred at D 0.275-0.3 h-1 which is identical to the results obtained in glucose-limited continuous cultures of this strain. Because galactose is not a repressing carbon source, this demonstrates that the Crabtree effect is not mediated by, or in any way related to glucose repression. Moreover, invertase and hexokinase I mRNA levels (both subject to glucose repression at the transcriptional level) were present when the yeast produced ethanol in galactose- and glucose-limited continuous cultures. In glucose-limited continuous cultures a decrease in alcohol dehydrogenase (I and II) mRNA levels and activity and phosphoglucomutase activity was observed with increasing dilution rates. In addition, at D 0.3 h-1, when the yeast produced ethanol, glucose-6-phosphate dehydrogenase and pyruvate decarboxylase were induced and a decrease in respiration was observed.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/8368013/Regulation_of_glycolytic_enzymes_and_the_Crabtree_effect_in_galactose_limited_continuous_cultures_of_Saccharomyces_cerevisiae_ L2 - https://doi.org/10.1002/yea.320090713 DB - PRIME DP - Unbound Medicine ER -