Tags

Type your tag names separated by a space and hit enter

The X-ray structure of the GCN4-bZIP bound to ATF/CREB site DNA shows the complex depends on DNA flexibility.
J Mol Biol. 1993 Sep 05; 233(1):139-54.JM

Abstract

The X-ray structure of the DNA binding domain of the yeast transcriptional activator protein GCN4 bound to a DNA fragment containing the sequence of the perfectly symmetrical ATF/CREB site has been solved to 3.0 A resolution. The architecture of this specific recognition complex supports the current model for bZIP proteins: a homodimer of parallel alpha-helices form an interhelix coiled-coil region via the leucine zipper, and the two N-terminal basic regions fit into the major groove of half sites on opposite sides of the DNA double helix. The structure shows that DNA flexibility plays the predominant role in the preservation of protein contacts with the symmetric ATF/CREB site (ATGACGTCAT) as compared to the pseudo-symmetric AP-1 target site (ATGACTCAT), overcoming the positional displacement of functional groups introduced by the additional G.C base-pair at the center of the ATF/CREB sequence.

Authors+Show Affiliations

König P
Institut für Molekularbiologie und Biophysik ETH-Höngerberg, Zürich, Switzerland.
Richmond TJ
No affiliation info available

MeSH

Activating Transcription FactorsAmino Acid SequenceBase SequenceBasic-Leucine Zipper Transcription FactorsBinding SitesBlood ProteinsCrystallizationCyclic AMP Response Element-Binding ProteinDNA-Binding ProteinsFungal ProteinsG-Box Binding FactorsGenes, SyntheticLeucine ZippersMacromolecular SubstancesModels, MolecularMolecular Sequence DataNucleic Acid ConformationOligodeoxyribonucleotidesPeptide FragmentsPlant ProteinsProtein ConformationProtein KinasesProtein Structure, SecondaryRecombinant ProteinsRegulatory Sequences, Nucleic AcidSaccharomyces cerevisiae ProteinsTranscription FactorsX-Ray Diffraction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8377181

Citation

König, P, and T J. Richmond. "The X-ray Structure of the GCN4-bZIP Bound to ATF/CREB Site DNA Shows the Complex Depends On DNA Flexibility." Journal of Molecular Biology, vol. 233, no. 1, 1993, pp. 139-54.
König P, Richmond TJ. The X-ray structure of the GCN4-bZIP bound to ATF/CREB site DNA shows the complex depends on DNA flexibility. J Mol Biol. 1993;233(1):139-54.
König, P., & Richmond, T. J. (1993). The X-ray structure of the GCN4-bZIP bound to ATF/CREB site DNA shows the complex depends on DNA flexibility. Journal of Molecular Biology, 233(1), 139-54.
König P, Richmond TJ. The X-ray Structure of the GCN4-bZIP Bound to ATF/CREB Site DNA Shows the Complex Depends On DNA Flexibility. J Mol Biol. 1993 Sep 5;233(1):139-54. PubMed PMID: 8377181.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The X-ray structure of the GCN4-bZIP bound to ATF/CREB site DNA shows the complex depends on DNA flexibility. AU - König,P, AU - Richmond,T J, PY - 1993/9/5/pubmed PY - 1993/9/5/medline PY - 1993/9/5/entrez SP - 139 EP - 54 JF - Journal of molecular biology JO - J Mol Biol VL - 233 IS - 1 N2 - The X-ray structure of the DNA binding domain of the yeast transcriptional activator protein GCN4 bound to a DNA fragment containing the sequence of the perfectly symmetrical ATF/CREB site has been solved to 3.0 A resolution. The architecture of this specific recognition complex supports the current model for bZIP proteins: a homodimer of parallel alpha-helices form an interhelix coiled-coil region via the leucine zipper, and the two N-terminal basic regions fit into the major groove of half sites on opposite sides of the DNA double helix. The structure shows that DNA flexibility plays the predominant role in the preservation of protein contacts with the symmetric ATF/CREB site (ATGACGTCAT) as compared to the pseudo-symmetric AP-1 target site (ATGACTCAT), overcoming the positional displacement of functional groups introduced by the additional G.C base-pair at the center of the ATF/CREB sequence. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/8377181/The_X_ray_structure_of_the_GCN4_bZIP_bound_to_ATF/CREB_site_DNA_shows_the_complex_depends_on_DNA_flexibility_ DB - PRIME DP - Unbound Medicine ER -