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The isocitrate dehydrogenase phosphorylation cycle: regulation and enzymology.
J Cell Biochem. 1993 Jan; 51(1):14-8.JC

Abstract

Isocitrate dehydrogenase (IDH) of Escherichia coli is regulated by phosphorylation and dephosphorylation. This phosphorylation cycle controls the flow of isocitrate through the glyoxylate bypass, a pathway which bypasses the CO2 evolving steps of the Krebs' cycle. IDH is phosphorylated at a single serine which resides in its active site. Phosphorylation blocks isocitrate binding, thereby inactivating IDH. The IDH phosphorylation cycle is catalyzed by a bifunctional protein kinase/phosphatase. The kinase and phosphatase reactions appear to be catalyzed at the same site and may share some catalytic steps. A variety of approaches have been used to examine the IDH phosphorylation cycle in the intact organism. The picture which has emerged is one of an exquisitely sensitive and flexible system which is capable of adapting efficiently to the environment both inside and outside the cell.

Authors+Show Affiliations

Department of Biochemistry, University of Minnesota, Minneapolis 55455.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.
Review

Language

eng

PubMed ID

8381789

Citation

LaPorte, D C.. "The Isocitrate Dehydrogenase Phosphorylation Cycle: Regulation and Enzymology." Journal of Cellular Biochemistry, vol. 51, no. 1, 1993, pp. 14-8.
LaPorte DC. The isocitrate dehydrogenase phosphorylation cycle: regulation and enzymology. J Cell Biochem. 1993;51(1):14-8.
LaPorte, D. C. (1993). The isocitrate dehydrogenase phosphorylation cycle: regulation and enzymology. Journal of Cellular Biochemistry, 51(1), 14-8.
LaPorte DC. The Isocitrate Dehydrogenase Phosphorylation Cycle: Regulation and Enzymology. J Cell Biochem. 1993;51(1):14-8. PubMed PMID: 8381789.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The isocitrate dehydrogenase phosphorylation cycle: regulation and enzymology. A1 - LaPorte,D C, PY - 1993/1/1/pubmed PY - 1993/1/1/medline PY - 1993/1/1/entrez SP - 14 EP - 8 JF - Journal of cellular biochemistry JO - J Cell Biochem VL - 51 IS - 1 N2 - Isocitrate dehydrogenase (IDH) of Escherichia coli is regulated by phosphorylation and dephosphorylation. This phosphorylation cycle controls the flow of isocitrate through the glyoxylate bypass, a pathway which bypasses the CO2 evolving steps of the Krebs' cycle. IDH is phosphorylated at a single serine which resides in its active site. Phosphorylation blocks isocitrate binding, thereby inactivating IDH. The IDH phosphorylation cycle is catalyzed by a bifunctional protein kinase/phosphatase. The kinase and phosphatase reactions appear to be catalyzed at the same site and may share some catalytic steps. A variety of approaches have been used to examine the IDH phosphorylation cycle in the intact organism. The picture which has emerged is one of an exquisitely sensitive and flexible system which is capable of adapting efficiently to the environment both inside and outside the cell. SN - 0730-2312 UR - https://www.unboundmedicine.com/medline/citation/8381789/The_isocitrate_dehydrogenase_phosphorylation_cycle:_regulation_and_enzymology_ L2 - https://doi.org/10.1002/jcb.240510104 DB - PRIME DP - Unbound Medicine ER -