TY - JOUR T1 - Effect of glutathione on aconitase in Escherichia coli. AU - Gardner,P R, AU - Fridovich,I, PY - 1993/2/15/pubmed PY - 1993/2/15/medline PY - 1993/2/15/entrez SP - 98 EP - 102 JF - Archives of biochemistry and biophysics JO - Arch Biochem Biophys VL - 301 IS - 1 N2 - The effect of glutathione (GSH) on the superoxide-sensitive [4Fe-4S]-containing aconitase of Escherichia coli was explored. A mutant deficient in GSH biosynthesis, designated gshA, grew slower in a defined medium than did the parental strain and this effect was more pronounced when succinate was supplied as the carbon source in place of glucose. This suggested that the citric acid cycle was compromised in the gshA strain. Aconitase activity was approximately 25% lower in GSH-deficient cells growing on either glucose or succinate, and was lower still in strains producing less superoxide dismutase. Addition of GSH to the medium stimulated growth of the gshA strain on succinate. It also elevated the aconitase activity in the presence of chloramphenicol, which was added to block protein synthesis. Dithiothreitol and 2-mercaptoethanol were much less effective in this regard than was GSH. Exposure of cultures to 4.2 atm O2 caused a rapid decline in aconitase activity and this was the case in both GSH-proficient and GSH-deficient E. coli; however, the reactivation which was seen when the hyperoxic exposure was terminated was significantly impaired in the gshA strain. There is a dynamic balance between inactivation of aconitase by superoxide and reactivation by Fe(II) and this balance is altered in GSH-deficient E. coli. GSH may facilitate reactivation of aconitase, and of other [4Fe-4S]-containing dehydratases, by increasing the rate of transfer of Fe(II) to the [3Fe-4S] site. SN - 0003-9861 UR - https://www.unboundmedicine.com/medline/citation/8382910/Effect_of_glutathione_on_aconitase_in_Escherichia_coli_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9861(83)71120-3 DB - PRIME DP - Unbound Medicine ER -